Construct: ORF TRCN0000491517
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002740.1_s317c1
- Derived from:
- ccsbBroadEn_13603
- DNA Barcode:
- TTGCGCGGGTTATACTACTGTGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VWA2 (340706)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491517
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340706 | VWA2 | von Willebrand factor A dom... | XM_017016180.1 | 90.8% | 90% | (many diffs) |
| 2 | human | 340706 | VWA2 | von Willebrand factor A dom... | NM_001272046.2 | 54.2% | 48.9% | (many diffs) |
| 3 | human | 340706 | VWA2 | von Willebrand factor A dom... | NM_001320804.1 | 54.2% | 48.9% | (many diffs) |
| 4 | human | 340706 | VWA2 | von Willebrand factor A dom... | XM_011539754.2 | 54% | 48.7% | (many diffs) |
| 5 | human | 340706 | VWA2 | von Willebrand factor A dom... | XM_017016179.2 | 53.8% | 48.6% | (many diffs) |
| 6 | human | 340706 | VWA2 | von Willebrand factor A dom... | XM_017016178.1 | 53.7% | 48.5% | (many diffs) |
| 7 | human | 340706 | VWA2 | von Willebrand factor A dom... | XM_017016177.1 | 53.5% | 48.3% | (many diffs) |
| 8 | human | 340706 | VWA2 | von Willebrand factor A dom... | XM_017016181.1 | 13.5% | 9.9% | 1_946del;1245_1246ins964 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1332
- ORF length:
- 1263
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggcacat gtgttccaga aggactggac ggctaccagt gcctctgccc 121 gctggccttt ggaggggagg ctaactgtgg tagccctgaa gctgagcctg gaatgcaggg 181 tcgacctcct cttcctgctg gacagctctg cgggcaccac tctggacggc ttcctgcggg 241 ccaaagtctt cgtgaagcgg tttgtgcggg ccgtgctgag cgaggactct cgggcccgag 301 tgggtgtggc cacatacagc agggagctgc tggtggcggt gcctgtgggg gagtaccagg 361 atgtgcctga cctggtctgg agcctcgatg gcattccctt ccgtggtggc cccaccctga 421 cgggcagtgc cttgcggcag gcggcagagc gtggcttcgg gagcgccacc aggacaggcc 481 aggaccggcc acgtagagtg gtggttttgc tcactgagtc acactccgag gatgaggttg 541 cgggcccagc gcgtcacgca agggcgcgag agctgctcct gctgggtgta ggcagtgagg 601 ccgtgcgggc agagctggag gagatcacag gcagcccaaa gcatgtgatg gtctactcgg 661 atcctcagga tctgttcaac caaatccctg agctgcaggg gaagctgtgc agccggcagc 721 ggccagggtg ccggacacaa gccctggacc tcgtcttcat gttggacacc tctgcctcag 781 tagggcccga gaattttgct cagatgcaga gctttgtgag aagctgtgcc ctccagtttg 841 aggtgaaccc tgacgtgaca caggtcggcc tggtggtgta tggcagccag gtgcagactg 901 ccttcgggct ggacaccaaa cccacccggg ctgcgatgct gcgggccatt agccaggccc 961 cctacctagg tggggtgggc tcagccggca ccgcccTGCT GCACATCTAT GACAAAGTGA 1021 TGACCGTCCA GAGGGGTGCC CGGCCTGGTG TCCCCAAAGC TGTGGTGGTG CTCACAGGCG 1081 GGAGAGGCGC AGAGGATGCA GCCGTTCCTG CCCAGAAGCT GAGGAACAAT GGCATCTCTG 1141 TCTTGGTCGT GGGCGTGGGG CCTGTCCTAA GTGAGGGTCT GCGGAGGCTT GCAGGTCCCC 1201 GGGATTCCCT GATCCACGTG GCAGCTTACG CCGACCTGCG GTACCACCAG GACGTGCTCA 1261 TTGAGTGGCT GTGTGGAGGT GAGTGGGGGA ATCCACACCC TCAGGGCTGC CCCCATGGCA 1321 GGCCCTCAGC CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1381 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1441 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTG CGCGGGTTAT ACTACTGTGA 1501 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t