Construct: ORF TRCN0000491549
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019800.2_s317c1
- DNA Barcode:
- AAACCACCCTGCTGTGAGTCTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DRD3 (1814)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491549
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1814 | DRD3 | dopamine receptor D3 | NM_001282563.2 | 99.7% | 99.5% | 15C>T;25G>A;1200_1201insG |
| 2 | human | 1814 | DRD3 | dopamine receptor D3 | NM_001290809.1 | 99.7% | 99.5% | 15C>T;25G>A;1200_1201insG |
| 3 | human | 1814 | DRD3 | dopamine receptor D3 | NM_000796.6 | 99.6% | 99.5% | (many diffs) |
| 4 | human | 1814 | DRD3 | dopamine receptor D3 | XM_017005829.1 | 99.6% | 99.5% | (many diffs) |
| 5 | human | 1814 | DRD3 | dopamine receptor D3 | NM_033663.5 | 91.5% | 91% | (many diffs) |
| 6 | mouse | 13490 | Drd3 | dopamine receptor D3 | NM_007877.2 | 77.9% | 81% | (many diffs) |
| 7 | mouse | 13490 | Drd3 | dopamine receptor D3 | XM_006521777.2 | 72.8% | 75.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1272
- ORF length:
- 1203
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcatctctg agtcagctga gtagccacct gaactacacc tgtggggcag 121 agaactccac aggtgccagc caggcccgcc cacatgccta ctatgccctc tcctactgcg 181 cgctcatcct ggccatcgtc ttcggcaatg gcctggtgtg catggctgtg ctgaaggagc 241 gggccctgca gactaccacc aactacttag tagtgagcct ggctgtggca gacttgctgg 301 tggccacctt ggtgatgccc tgggtggtat acctggaggt gacaggtgga gtctggaatt 361 tcagccgcat ttgctgtgat gtttttgtca ccctggatgt catgatgtgt acagccagca 421 tccttaatct ctgtgccatc agcatagaca ggtacactgc agtggtcatg cccgttcact 481 accagcatgg cacgggacag agctcctgtc ggcgcgtggc cctcatgatc acggccgtct 541 gggtactggc ctttgctgtg tcctgccctc ttctgtttgg ctttaatacc acaggggacc 601 ccactgtctg ctccatctcc aaccctgatt ttgtcatcta ctcttcagtg gtgtccttct 661 acctgccctt tggagtgact gtccttgtct atgccagaat ctatgtggtg ctgaaacaaa 721 ggagacggaa aaggatcctc actcgacaga acagtcagtg caacagtgtc aggcctggct 781 tcccccaaca aaccctctct cctgacccgg cacatctgga gctgaagcgt tactacagca 841 tctgccagga cactgccttg ggtggaccag gcttccaaga aagaggagga gagttgaaaa 901 gagaggagaa gactcggaat tccctgagtc ccaccatagc gcccaagctc agcttagaag 961 ttcGAAAACT CAGCAATGGC AGATTATCGA CATCTTTGAA GCTGGGGCCC CTGCAACCTC 1021 GGGGAGTGCC ACTTCGGGAG AAGAAGGCAA CCCAAATGGT GGCCATTGTG CTTGGGGCCT 1081 TCATTGTCTG CTGGCTGCCC TTCTTCTTGA CCCATGTTCT CAATACCCAC TGCCAGACAT 1141 GCCACGTGTC CCCAGAGCTT TACAGTGCCA CGACATGGCT GGGCTACGTG AATAGCGCCC 1201 TCAACCCTGT GATCTATACC ACCTTCAATA TCGAGTTCCG GAAAGCCTTC CTCAAGATCC 1261 TGTCTTGCGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1321 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1381 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAACCA CCCTGCTGTG AGTCTTTGAC 1441 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt