Construct: ORF TRCN0000491557
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003876.1_s317c1
- Derived from:
- ccsbBroadEn_00003
- DNA Barcode:
- GCCATACTGGAACATTCCGATAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABAT (18)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491557
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 18 | ABAT | 4-aminobutyrate aminotransf... | NM_000663.4 | 100% | 100% | |
| 2 | human | 18 | ABAT | 4-aminobutyrate aminotransf... | NM_001127448.2 | 100% | 100% | |
| 3 | human | 18 | ABAT | 4-aminobutyrate aminotransf... | NM_020686.6 | 100% | 100% | |
| 4 | human | 18 | ABAT | 4-aminobutyrate aminotransf... | XM_011522400.2 | 100% | 100% | |
| 5 | human | 18 | ABAT | 4-aminobutyrate aminotransf... | XM_011522401.2 | 100% | 100% | |
| 6 | mouse | 268860 | Abat | 4-aminobutyrate aminotransf... | NM_172961.3 | 87.2% | 91.2% | (many diffs) |
| 7 | mouse | 268860 | Abat | 4-aminobutyrate aminotransf... | NM_001170978.1 | 77% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1566
- ORF length:
- 1500
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctccatgttg ctcgcccagc gcctggcctg cagcttccag cacagctacc 121 gcctgctggt gcctggatcc agacacatta gtcaagctgc agccaaagtc gacgttgaat 181 ttgattatga tgggcctctg atgaagacgg aagtcccagg gcctagatct caggagttaa 241 tgaaacagct gaatataatt cagaatgcag aggctgtgca ttttttctgc aattacgaag 301 agagccgagg caattacctg gttgatgtgg acggcaaccg aatgctggat ctttattccc 361 agatctcctc tgtccccata ggttacagcc accccgccct gctgaaactc atccaacagc 421 ctcaaaatgc gagcatgttt gtcaacagac ccgccctcgg aatcctgcct ccggagaact 481 ttgtggagaa gctccggcag tccttgctct cggtggctcc caaagggatg tcccagctca 541 tcaccatggc ctgcggctcc tgctccaatg aaaacgcctt aaagaccatc ttcatgtggt 601 accggagcaa ggaaagaggg cagaggggct tctcccagga ggagctggag acgtgcatga 661 ttaaccaggc ccctggctgc cccgactaca gcatcctctc cttcatgggc gcgttccatg 721 ggaggaccat gggttgctta gcgaccacgc actctaaagc cattcacaag atcgacatcc 781 cttcctttga ctggcccatc gcaccgttcc cacggctgaa ataccctctg gaagagtttg 841 tgaaagagaa ccaacaggag gaggcccgct gtctggaaga ggtggaggat ctgattgtga 901 aatatcggaa aaagaagaag acggtggccg ggatcatcgt ggagcccatc cagtccgagg 961 gtggagacaa ccacgcatcc gatgacttct ttcggaagct gagagacatc gccaggaagc 1021 atggctgcgc cttcttggtg gacgaggtcc agaccggagg aggctgcacg ggcaagttct 1081 gggcccatga gcactggggc ctggatgacc cagcagacgt gatgaccttc agcaagaaga 1141 tgatgactgg gggcttcttc cacaaggagg agttcaggcc taatgctccc taccggatct 1201 tcaacacctG GCTGGGGGAC CCGTCCAAGA ACCTGTTGCT GGCTGAGGTC ATCAACATCA 1261 TCAAGCGGGA GGACCTGCTA AATAATGCAG CCCATGCCGG GAAGGCCCTG CTCACAGGAC 1321 TGCTGGACCT CCAGGCCCGG TACCCCCAGT TCATCAGCAG GGTGAGAGGA CGAGGCACCT 1381 TTTGCTCCTT CGATACTCCC GATGATTCCA TACGGAATAA GCTCATTTTA ATTGCCAGAA 1441 ACAAAGGTGT GGTGTTGGGT GGCTGTGGTG ACAAATCCAT TCGTTTCCGT CCCACGCTGG 1501 TCTTCAGGGA TCACCACGCT CACCTGTTCC TCAATATTTT CAGTGACATC TTAGCAGACT 1561 TCAAGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1621 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1681 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCCATACTG GAACATTCCG ATAACACGCG 1741 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt