Construct: ORF TRCN0000491558
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009130.2_s317c1
- Derived from:
- ccsbBroadEn_01695
- DNA Barcode:
- TGCAATAGGCCTATGCTGTGCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNP1 (7141)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491558
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7141 | TNP1 | transition protein 1 | NM_003284.4 | 100% | 100% | |
| 2 | mouse | 21958 | Tnp1 | transition protein 1 | NM_009407.2 | 89% | 87.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 194
- ORF end:
- 359
- ORF length:
- 165
- Sequence:
-
1 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 61 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 121 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 181 CAAAAAAGTT GGCATGTCGA CCAGCCGCAA ATTAAAGAGT CATGGCATGA GGAGGAGCAA 241 GAGCCGATCT CCTCACAAGG GAGTCAAGAG AGGTGGCAGC AAAAGAAAAT ACCGTAAGGG 301 CAACCTGAAA AGTAGGAAAC GGGGCGATGA CGCCAATCGC AATTACCGCT CCCACTTGTG 361 CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG 421 TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT 481 TTATATATCT TGTGGAAAGG ACGATGCAAT AGGCCTATGC TGTGCTTGAC GCGTTAAGTC 541 gacaatcaac ctctggatta caaaatttgt gaaagatt