Construct: ORF TRCN0000491566
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020086.2_s317c1
- DNA Barcode:
- TGCCAGATTATATCCTCATCCTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IRAK1 (3654)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491566
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | NM_001569.4 | 99.9% | 99.8% | 2136_2137insG |
| 2 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | NM_001025242.2 | 95.7% | 95.6% | 1536_1537ins90;2046_2047insG |
| 3 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | XM_005274668.4 | 92.3% | 92.2% | 136_213del;1614_1615ins90;2124_2125insG |
| 4 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | NM_001025243.2 | 88.8% | 88.7% | 1301_1302ins237;1899_1900insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2208
- ORF length:
- 2139
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggccgggggg ccgggcccgg gggagcccgc agcccccggc gcccagcact 121 tcttgtacga ggtgccgccc tgggtcatgt gccgcttcta caaagtgatg gacgccctgg 181 agcccgccga ctggtgccag ttcgccgccc tgatcgtgcg cgaccagacc gagctgcggc 241 tgtgcgagcg ctccgggcag cgcacggcca gcgtcctgtg gccctggatc aaccgcaacg 301 cccgtgtggc cgacctcgtg cacatcctca cgcacctgca gctgctccgt gcgcgggaca 361 tcatcacagc ctggcaccct cccgccccgc ttccgtcccc aggcaccact gccccgaggc 421 ccagcagcat ccctgcaccc gccgaggccg aggcctggag cccccggaag ttgccatcct 481 cagcctccac cttcctctcc ccagcttttc caggctccca gacccattca gggcctgagc 541 tcggcctggt cccaagccct gcttccctgt ggcctccacc gccatctcca gccccttctt 601 ctaccaagcc aggcccagag agctcagtgt ccctcctgca gggagcccgc ccctttccgt 661 tttgctggcc cctctgtgag atttcccggg gcacccacaa cttctcggag gagctcaaga 721 tcggggaggg tggctttggg tgcgtgtacc gggcggtgat gaggaacacg gtgtatgctg 781 tgaagaggct gaaggagaac gctgacctgg agtggactgc agtgaagcag agcttcctga 841 ccgaggtgga gcagctgtcc aggtttcgtc acccaaacat tgtggacttt gctggctact 901 gtgctcagaa cggcttctac tgcctggtgt acggcttcct gcccaacggc tccctggagg 961 accgtctcca ctgccagacc caggcctgcc cacctctctc ctggcctcag cgactggaca 1021 tccttctggg tacagcccgg gcaattcagt ttctacatca ggacagcccc agcctcatcc 1081 atggagacat caagagttcc aacgtccttc tggatgagag gctgacaccc aagctgggag 1141 actttggcct ggcccggttc agccgctttg ccgggtccag ccccagccag agcagcatgg 1201 tggcccggac acagacagtg cggggcaccc tggcctacct gcccgaggag tacatcaaga 1261 cgggaaggct ggctgtggac acggacacct tcagctttgg ggtggtagtg ctagagacct 1321 tggctggtca gagggctgtg aagacgcacg gtgccaggac caagtatctg aaagacctgg 1381 tggaagagga ggctgaggag gctggagtgg ctttgagaag cacccagagc acactgcaag 1441 caggtctggc tgcagatgcc tgggctgctc ccatcgccat gcagatctac aagaagcacc 1501 tggaccccag gcccgggccc tgcccacctg agctgggcct gggcctgggc cagctggcct 1561 gctgctgcct gcaccgccgg gccaaaagga ggcctcctat gacccaggtg tacgagaggc 1621 tagagaagct gcaggcagtg gtggcggggg tgcccgggca ttcggaggcc gccagctgca 1681 tccccccttc cccgcaggag aactcctacg tgtccagcac tggcagagcc cacagtgggg 1741 ctgctccatg gcagcccctg gcagcgccat caggagccag tgcccaggca gcagagcagc 1801 tgcagagagg ccccaaccag cccgtggaga gtgacgagag cctaggcggc ctctctgctg 1861 ccctgcgctc ctggcacttg actccaagct gccctctgga cccagcaccc cTCAGGGAGG 1921 CCGGCTGTCC TCAGGGGGAC ACGGCAGGAG AATCGAGCTG GGGGAGTGGC CCAGGATCCC 1981 GGCCCACAGC CGTGGAAGGA CTGGCCCTTG GCAGCTCTGC ATCATCGTCG TCAGAGCCAC 2041 CGCAGATTAT CATCAACCCT GCCCGACAGA AGATGGTCCA GAAGCTGGCC CTGTACGAGG 2101 ATGGGGCCCT GGACAGCCTG CAGCTGCTGT CGTCCAGCTC CCTCCCAGGC TTGGGCCTGG 2161 AACAGGACAG GCAGGGGCCC GAAGAAAGTG ATGAATTTCA GAGCGACCCA GCTTTCTTGT 2221 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2281 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2341 GAAAGGACGA TGCCAGATTA TATCCTCATC CTCGACGCGT TAAGTCgaca atcaacctct 2401 ggattacaaa atttgtgaaa gatt