Construct: ORF TRCN0000491568

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019730.2_s317c1
DNA Barcode:
ACAGTCCCGTAAACGATTCTAGGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALDH18A1 (5832)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491568
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323413.2 99.9% 99.8% 2385_2386insG
2 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323414.2 99.9% 99.8% 2385_2386insG
3 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_002860.4 99.9% 99.8% 2385_2386insG
4 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001017423.2 99.7% 99.6% 711_712insGTAAAT;2379_2380insG
5 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323415.2 99.7% 99.6% 711_712insGTAAAT;2379_2380insG
6 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... XM_024448094.1 95.8% 95.7% 1800_1901del;2487_2488insG
7 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... XM_024448095.1 95.8% 95.7% 1800_1901del;2487_2488insG
8 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... XM_024448096.1 95.6% 95.5% 711_712insGTAAAT;1794_1895del;2481_2482insG
9 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323417.2 95.5% 95.4% 452_453ins105;2280_2281insG
10 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323412.2 86% 85.9% 0_1ins333;2052_2053insG
11 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323416.2 86% 85.9% 0_1ins333;2052_2053insG
12 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323418.2 85.7% 85.6% 0_1ins333;378_379insGTAAAT;2046_2047insG
13 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... XM_024448097.1 82.4% 82.4% 0_1ins333;1467_1568del;2154_2155insG
14 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323419.2 73.3% 73.2% 0_1ins636;1749_1750insG
15 mouse 56454 Aldh18a1 aldehyde dehydrogenase 18 f... NM_019698.2 87.7% 95.3% (many diffs)
16 mouse 56454 Aldh18a1 aldehyde dehydrogenase 18 f... NM_153554.2 87.4% 95.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2460
ORF length:
2388
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgttgagt caagtttacc gctgtgggtt ccagcccttc aaccaacatc
121ttctgccctg ggtcaagtgt acaaccgtct tcagatctca ttgtatccag ccttcagtca
181tcagacatgt tcgttcttgg agcaacatcc cgtttatcac tgtacccctc agtcgtacac
241atggcaagtc cttcgcccac cgcagtgagc tgaagcatgc caagagaatc gtggtgaagc
301tcggcagtgc cgtggtgacc cgaggggatg aatgtggcct ggccctgggg cgcttggcat
361ctattgttga gcaggtatca gtgctgcaga atcagggcag agagatgatg ctggtgacca
421gtggagccgt agcctttggc aaacaacgct tgcgccatga gatccttctg tctcagagcg
481tgcggcaggc cctccactcg gggcagaacc agctgaaaga aatggcaatt ccagtcttag
541aggcacgagc ctgtgcagct gccggacaga gtgggctgat ggccttgtat gaggctatgt
601ttacccagta cagcatctgt gctgcccaga ttttggtgac caatttggat ttccatgatg
661agcagaagcg ccggaacctc aatggaacac ttcatgaact ccttagaatg aacattgtcc
721ccattgtcaa cacaaatgat gctgttgtcc ccccagctga gcccaacagt gacctgcagg
781gggtaaatgt tattagtgtt aaagataatg atagcctggc tgcccgactg gctgtggaaa
841tgaaaactga tctcttgatt gttctttcag atgtagaagg cctttttgac agccccccag
901gttcagatga tgcaaagctt attgatatat tttatcccgg agatcagcag tctgtgacat
961ttggaaccaa gtctagagtg ggaatgggtg gcatggaagc caaggtgaaa gcagccctct
1021gggctttgca aggtggcact tctgttgtta ttgccaatgg aacccaccca aaggtgtctg
1081ggcacgtcat cacagacatt gtggagggga agaaagttgg taccttcttt tcagaagtaa
1141agcctgcagg ccctactgtt gagcagcagg gagaaatggc gcgatctgga ggaaggatgt
1201tggccacctt ggaacctgag cagagagcag aaattatcca tcatctggct gatctgttga
1261cggaccagcg tgatgagatc ctgttagcca acaaaaaaga cttggaggag gcagagggga
1321gacttgcagc tcctctgctg aaacgtttaa gcctctccac atccaaattg aacagcctgg
1381ccatcggtct gcgacagatc gcagcctcct cccaggacag cgtgggacgt gttttgcgcc
1441gcacccgaat cgccaaaaac ttggaactgg aacaagtgac tgtcccaatt ggagttctgc
1501tggtgatctt tgaatctcgt cctgactgtc taccccaggt ggcagctttg gctatcgcaa
1561gtggcaatgg cttgttactc aaaggaggga aggaggctgc acacagcaac cggattctcc
1621acctcctgac ccaggaggct ctctcaatcc atggagtcaa ggaggccgtg caactggtga
1681ataccagaga agaagttgaa gatctttgcc gcctagacaa aatgatagat ctgatcattc
1741cacgtggctc ttcccagctg gtcagagaca tccagaaagc tgctaagggg attccagtga
1801tggggcacag cgaagggatc tgtcacatgt atgtggattc cgaggccagt gttgataagg
1861tcaccaggct agtcagagac tctaaatgtg aatatccagc tgcctgtaat gctttggaga
1921ctttgttaat ccaccgggat ctgctcagga caccattatt tgaccagatc attgatatgc
1981tgagagtgga acaggtaaaa attcatgcag gccccaaatt tgcctcctat ctgaccttca
2041gcccctccga agtgaagtca ctccgaactg agtatgggga cctggaatta tgcattgaag
2101tagtggacaa cgttcaggat gccattgacc acatccacaa gtatGGCAGC TCCCACACGG
2161ATGTCATCGT CACAGAGGAC GAAAACACAG CGGAGTTCTT CCTGCAGCAC GTAGACAGTG
2221CCTGTGTGTT CTGGAATGCC AGCACTCGCT TTTCTGATGG TTACCGCTTT GGACTGGGAG
2281CTGAAGTGGG AATCAGTACA TCGAGAATCC ACGCCCGGGG ACCAGTAGGA CTTGAGGGAC
2341TGCTTACTAC TAAGTGGCTG CTGCGAGGGA AGGACCACGT GGTCTCAGAT TTCTCAGAGC
2401ATGGAAGTTT AAAATATCTT CATGAGAACC TCCCTATTCC TCAGAGAAAC ACCAACGACC
2461CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
2521TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
2581ATATATCTTG TGGAAAGGAC GAACAGTCCC GTAAACGATT CTAGGCACGC GTTAAGTCga
2641caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)