Transcript: Human NM_001017423.2

Homo sapiens aldehyde dehydrogenase 18 family member A1 (ALDH18A1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
ALDH18A1 (5832)
Length:
3346
CDS:
144..2525

Additional Resources:

NCBI RefSeq record:
NM_001017423.2
NBCI Gene record:
ALDH18A1 (5832)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149056 TGCCAATGGAACCCACCCAA pXPR_003 AGG 991 42% 9 0.5246 ALDH18A1 ALDH18A1 77994
2 BRDN0001145444 GCAGCTTTGGCTATCGCAAG pXPR_003 TGG 1481 62% 13 -0.0609 ALDH18A1 ALDH18A1 77995
3 BRDN0001487061 ACTTGCCATGTGTACGACTG pXPR_003 AGG 161 7% 3 -0.1655 ALDH18A1 ALDH18A1 77996
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001017423.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000414958 GGCTAGTCAGAGACTCTAAAT pLKO_005 1933 CDS 100% 13.200 18.480 N ALDH18A1 n/a
2 TRCN0000064852 GCATCTATTGTTGAGCAGGTA pLKO.1 429 CDS 100% 2.640 2.112 N ALDH18A1 n/a
3 TRCN0000434520 ACTTTGCCAAGTCCAATTATC pLKO_005 2758 3UTR 100% 13.200 9.240 N ALDH18A1 n/a
4 TRCN0000455026 AGGCCGTGCAACTGGTGAATA pLKO_005 1729 CDS 100% 13.200 9.240 N ALDH18A1 n/a
5 TRCN0000413267 TTCACGTTAAACTTGTCTTAT pLKO_005 2563 3UTR 100% 13.200 9.240 N ALDH18A1 n/a
6 TRCN0000415272 TTCCGAGGCCAGTGTTGATAA pLKO_005 1904 CDS 100% 13.200 9.240 N ALDH18A1 n/a
7 TRCN0000421109 TTGGAGCAACATCCCGTTTAT pLKO_005 269 CDS 100% 13.200 9.240 N ALDH18A1 n/a
8 TRCN0000422978 ACTAGAGCCAGTCATCCTTAA pLKO_005 2884 3UTR 100% 10.800 7.560 N ALDH18A1 n/a
9 TRCN0000436081 GGAATCAGTACATCGAGAATC pLKO_005 2355 CDS 100% 10.800 7.560 N ALDH18A1 n/a
10 TRCN0000064850 CGGAACCTCAATGGAACACTT pLKO.1 744 CDS 100% 4.950 3.465 N ALDH18A1 n/a
11 TRCN0000064849 CCATTATTTGACCAGATCATT pLKO.1 2019 CDS 100% 5.625 3.375 N ALDH18A1 n/a
12 TRCN0000064848 GCCTTGTATGAGGCTATGTTT pLKO.1 654 CDS 100% 5.625 3.375 N ALDH18A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001017423.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491568 ACAGTCCCGTAAACGATTCTAGGC pLX_317 13.3% 99.7% 99.6% V5 711_712insGTAAAT;2379_2380insG n/a
2 ccsbBroadEn_14822 pDONR223 68.3% 98.8% 29.8% None (many diffs) n/a
3 ccsbBroad304_14822 pLX_304 0% 98.8% 29.8% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000475053 AGCACTCTCGGTAAAAATCCACGC pLX_317 17.5% 98.8% 29.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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