Construct: ORF TRCN0000491591
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021399.2_s317c1
- DNA Barcode:
- ACATCAATAAGTGTTTTATAATAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EPHA2 (1969)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491591
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1969 | EPHA2 | EPH receptor A2 | NM_001329090.2 | 41% | .4% | 1_1618del;1821C>T;2766_2767ins28 |
| 2 | human | 1969 | EPHA2 | EPH receptor A2 | NM_004431.5 | 38.8% | .4% | 1_1780del;1983C>T;2928_2929ins28 |
| 3 | mouse | 13836 | Epha2 | Eph receptor A2 | XM_006538528.3 | 47.6% | .5% | (many diffs) |
| 4 | mouse | 13836 | Epha2 | Eph receptor A2 | NM_010139.3 | 34.4% | .4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 175
- ORF end:
- 199
- ORF length:
- 24
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccaga tctaaccagc gtgcccgcca gtccccggag gacgtttact 121 tctccaagtc agaacaactg aagcccctga agacatacgt ggacccccac acatatgagg 181 accccaacca ggctgtgttg aagttcacta ccgagatcca tccatcctgt gtcactcggc 241 agaaggtgat cggagcagga gagtttgggg aggtgtacaa gggcatgctg aagacatcct 301 cggggaagaa ggaggtgccg gtggccatca agacgctgaa agccggctac acagagaagc 361 agcgagtgga cttccttggc gaggccggca tcatgggcca gttcagccac cacaacatca 421 tccgcctaga gggcgtcatc tccaaataca agcccatgat gatcatcact gagtacatgg 481 agaatggggc cctggacaag ttccttcggg agaaggatgg cgagttcagc gtgctgcagc 541 tggtgggcat gctgcggggc atcgcagctg gcatgaagta cctggccaac atgaactatg 601 tgcaccgtga cctggctgcc cgcaacatcc tcgtcaacag caacctggtc tgcaaggtgt 661 ctgactttgg cctgtcccgc gtgctggagg acgaccccga ggccacctac accaccagtg 721 gcggcaagat ccccatccgc tggaccgccc cggaggccat ttcctaccgg aagttcacct 781 ctgccagcga cgtgtggagc tttggcattg tcatgtggga ggtgatgacc tatggcgagc 841 ggccctactg ggagttgtcc aaccacgagg tgatgaaagc catcaatgat ggcttccggc 901 tccccacacc catggactgc ccctccgcca tctaccagct catgatgcag tgctggcagc 961 aggagcgtgc ccgccgcccc aagttcgctg acatcgtcag catcctggac aagctcattc 1021 gtgccCCTGA CTCCCTCAAG ACCCTGGCTG ACTTTGACCC CCGCGTGTCT ATCCGGCTCC 1081 CCAGCACGAG CGGCTCGGAG GGGGTGCCCT TCCGCACGGT GTCCGAGTGG CTGGAGTCCA 1141 TCAAGATGCA GCAGTATACG GAGCACTTCA TGGCGGCCGG CTACACTGCC ATCGAGAAGG 1201 TGGTGCAGAT GACCAACGAC GACATCAAGA GGATTGGGGT GCGGCTGCCC GGCCACCAGA 1261 AGCGCATCGC CTACAGCCTG CTGGGACTCA AGGACCAGGT GAACACTGTG GGGATCCCCA 1321 TCTGAGCCTC GACAGGGCCT GGAGCAGATC TAAGGGTGGG CGCGCCGACC CAGCTTTCTT 1381 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 1441 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 1501 TGGAAAGGAC GAACATCAAT AAGTGTTTTA TAATATACGC GTTAAGTCga caatcaacct 1561 ctggattaca aaatttgtga aagatt