Construct: ORF TRCN0000491600
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019860.2_s317c1
- DNA Barcode:
- GCTGCACTGCGAATTGGCCTGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCYL1 (57410)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491600
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_017018033.1 | 61.3% | 56.9% | (many diffs) |
| 2 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_024448620.1 | 60.3% | 56.1% | (many diffs) |
| 3 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_005274120.4 | 54.3% | 54.3% | 1_1068del;2343_2344insG |
| 4 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_005274121.4 | 54.2% | 54.2% | 1_1068del;1957_1958insAGC;2340_2341insG |
| 5 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | NM_020680.4 | 52.5% | 49% | 1_1068del;2248_2302del;2399_2424delinsG |
| 6 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_005274118.4 | 52.4% | 48.8% | (many diffs) |
| 7 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_017018031.2 | 52.2% | 52.1% | 1_1068del;1814_1815ins51;2292_2293insG |
| 8 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | NM_001048218.2 | 50.4% | 46.9% | (many diffs) |
| 9 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_017018030.2 | 50.3% | 46.8% | (many diffs) |
| 10 | human | 57410 | SCYL1 | SCY1 like pseudokinase 1 | XM_024448619.1 | 50.1% | 46.7% | (many diffs) |
| 11 | mouse | 78891 | Scyl1 | SCY1-like 1 (S. cerevisiae) | NM_023912.2 | 44.5% | 43.2% | (many diffs) |
| 12 | mouse | 78891 | Scyl1 | SCY1-like 1 (S. cerevisiae) | XM_006531856.2 | 44.4% | 43% | (many diffs) |
| 13 | mouse | 78891 | Scyl1 | SCY1-like 1 (S. cerevisiae) | XM_006531857.2 | 43.1% | 41.9% | (many diffs) |
| 14 | mouse | 78891 | Scyl1 | SCY1-like 1 (S. cerevisiae) | XR_388273.2 | 22.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1347
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gttctcatcc actgaccggg ccatgcgcat ccgcctcctg cagcagatgg 121 agcagttcat ccagtacctt gacgagccaa cagtcaacac ccagatcttc ccccacgtcg 181 tacatggctt cctggacacc aaccctgcca tccgggagca gacggtcaag tccatgctgc 241 tcctggcccc aaagctgaac gaggccaacc tcaatgtgga gctgatgaag cactttgcac 301 ggctacaggc caaggatgaa cagggcccca tccgctgcaa caccacagtc tgcctgggca 361 aaatcggctc ctacctcagt gctagcacca gacacagggt ccttacctct gccttcagcc 421 gagccactag ggacccgttt gcaccgtccc gggttgcggg tgtcctgggc tttgctgcca 481 cccacaacct ctactcaatg aacgactgtg cccagaagat cctgcctgtg ctctgcggtc 541 tcactgtaga tcctgagaaa tccgtgcgag accaggcctt caaggccatt cggagcttcc 601 tgtccaaatt ggagtctgtg tcggaggacc cgacccagct ggaggaagtg gagaaggatg 661 tccatgcagc ctccagccct ggcatgggag gagccgcagc tagctgggca ggctgggccg 721 tgaccggggt ctcctcactc acctccaagc tgatccgttc gcacccaacc actgccccaa 781 cagaaaccaa cattccccaa agacccacgc ctgaaggagt tcctgcccca gcccccaccc 841 ctgttcctgc cacccctaca acctcaggcc actgggagac gcaggaggag gacaaggaca 901 cagcagagga cagcagcact gctgacagat gggacgacga agactggggc agcctggagc 961 aggaggccga gtctgtgctg gcccagcagg acgactggag caccgggggc caagtgagcc 1021 gtgctagtca ggtcagcaac TCCGACCACA AATCCTCCAA ATCCCCAGAG TCCGACTGGA 1081 GCAGCTGGGA AGCTGAGGGC TCCTGGGAAC AGGGCTGGCA GGAGCCAAGC TCCCAGGAGC 1141 CACCTCCTGA CGGTACACGG CTGGCCAGCG AGTATAACTG GGGTGGCCCA GAGTCCAGCG 1201 ACAAGGGCGA CCCCTTCGCT ACCCTGTCTG CACGTCCCAG CACCCAGGAC AGGTCAAGGC 1261 TGAGCTGGCC CGGAAGAAGC GCGAGGAGCG GCGGCGGGAG ATGGAGGCCA AACGCGCCGA 1321 GAGGAAGGTG GCCAAGGGCC CCAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1381 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1441 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CTGCACTGCG 1501 AATTGGCCTG AATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1561 att