Construct: ORF TRCN0000491603
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011311.1_s317c1
- Derived from:
- ccsbBroadEn_03816
- DNA Barcode:
- TCTGGCGAGGCCCAATCTCTTCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPC25 (57405)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491603
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57405 | SPC25 | SPC25 component of NDC80 ki... | NM_020675.4 | 100% | 100% | |
| 2 | human | 57405 | SPC25 | SPC25 component of NDC80 ki... | XM_011511516.2 | 82.4% | 82.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 738
- ORF length:
- 672
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt agaggacgaa ctggcacttt tcgataaaag cataaatgaa ttttggaata 121 aattcaaaag tacggacacc tcctgtcaga tggcgggact aagagatacc tacaaggatt 181 ccatcaaagc atttgcagaa aagctgtctg tgaaattaaa ggaagaagaa cgaatggttg 241 agatgtttct ggaatatcaa aatcagatca gcaggcaaaa taagctcatt caagaaaaaa 301 aggataactt gttaaaattg attgctgaag taaaaggcaa aaagcaggaa ttGGAAGTAC 361 TGACTGCAAA TATCCAGGAT CTTAAGGAAG AATATTCTAG GAAGAAGGAA ACTATTTCTA 421 CTGCTAATAA AGCGAATGCA GAGAGGTTGA AAAGGCTGCA GAAATCTGCA GACTTGTATA 481 AAGATCGACT TGGACTAGAA ATTCGAAAAA TTTATGGTGA GAAATTGCAG TTTATTTTCA 541 CTAATATTGA CCCTAAGAAT CCTGAGAGCC CATTTATGTT TTCCTTACAT CTCAATGAAG 601 CAAGGGACTA TGAAGTGTCA GATAGTGCCC CTCATCTTGA GGGCCTAGCA GAATTTCAAG 661 AGAATGTAAG GAAGACCAAC AATTTTTCAG CTTTTCTTGC CAATGTTCGG AAAGCTTTTA 721 CTGCCACGGT TTATAATTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 781 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 841 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCTGGCG AGGCCCAATC 901 TCTTCGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt