Construct: ORF TRCN0000491606
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004633.2_s317c1
- Derived from:
- ccsbBroadEn_13620
- DNA Barcode:
- AAGACATGTAACTTTTGGGATGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C22orf34 (348645)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491606
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 348645 | C22orf34 | chromosome 22 open reading ... | NM_001289922.3 | 24.4% | 17.7% | (many diffs) |
| 2 | human | 348645 | C22orf34 | chromosome 22 open reading ... | XM_024452238.1 | 11.4% | 10.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 224
- ORF end:
- 338
- ORF length:
- 114
- Sequence:
-
1 GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA 61 GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC 121 ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC 181 TAGCATCGAT TGATCAACAA GTTTGTACAA AAAAGTTGGC ACCATGCCCA TCCTCCAGGC 241 ACTGTGCCTC CTCCCAAAGG TCAGCACCCC GAGCATCACT GTGCTCTCCC CACAAAGGTC 301 AGCAGCCCCG AGCATCACTG TGCCCTCCCC ACAAAGGTTG CCAACTTTCT TGTACAAAGT 361 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 421 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 481 CGAAAGACAT GTAACTTTTG GGATGCAACG CGTTAAGTCg acaatcaacc tctggattac 541 aaaatttgtg aaagatt