Construct: ORF TRCN0000491611
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012260.1_s317c1
- Derived from:
- ccsbBroadEn_13905
- DNA Barcode:
- CAACTGATAGAAATCAATCTATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MXI1 (4601)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491611
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 4601 | MXI1 | MAX interactor 1, dimerizat... | NM_005962.5 | 92.7% | 92.7% | (many diffs) |
2 | human | 4601 | MXI1 | MAX interactor 1, dimerizat... | NM_130439.3 | 79.3% | 79.3% | 1_183del |
3 | human | 4601 | MXI1 | MAX interactor 1, dimerizat... | NM_001008541.1 | 77.7% | 77.7% | 0_1ins126;97_98ins30 |
4 | mouse | 17859 | Mxi1 | MAX interactor 1, dimerizat... | NM_010847.3 | 85.1% | 89.3% | (many diffs) |
5 | mouse | 17859 | Mxi1 | MAX interactor 1, dimerizat... | XM_017318079.1 | 81.6% | 82.9% | (many diffs) |
6 | mouse | 17859 | Mxi1 | MAX interactor 1, dimerizat... | NM_001008543.2 | 75% | 78.6% | (many diffs) |
7 | mouse | 17859 | Mxi1 | MAX interactor 1, dimerizat... | XM_006526738.1 | 75% | 78.6% | (many diffs) |
8 | mouse | 17859 | Mxi1 | MAX interactor 1, dimerizat... | XM_006526739.1 | 75% | 78.6% | (many diffs) |
9 | mouse | 17859 | Mxi1 | MAX interactor 1, dimerizat... | XM_006526740.1 | 75% | 78.6% | (many diffs) |
10 | mouse | 17859 | Mxi1 | MAX interactor 1, dimerizat... | NM_001008542.2 | 73.3% | 76.6% | (many diffs) |
11 | mouse | 17859 | Mxi1 | MAX interactor 1, dimerizat... | XM_006526735.3 | 70.1% | 73.2% | (many diffs) |
12 | mouse | 17859 | Mxi1 | MAX interactor 1, dimerizat... | XM_006526741.2 | 64.2% | 67.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 768
- ORF length:
- 702
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaagcacatc aacacttttc tgcagaacgt gcagattctg ctcgaggccg 121 ccagctacct ggagcagatc gagaaagaaa acaaaaagtg tgaacatggc tacgcctctt 181 cattcccgtc catgccgagc ccccgactgc agcattcaaa gcccccacgg aggttgagcc 241 gggcacagaa acacagcagc gggagcagca acaccagcac tgccaacaga tctacacaca 301 atgagctgga aaagaatcga cgagctcatc TGCGCCTTTG TTTAGAACGC TTAAAAGTTC 361 TGATTCCACT AGGACCAGAC TGCACCCGGC ACACAACACT TGGTTTGCTC AACAAAGCCA 421 AAGCACACAT CAAGAAACTT GAAGAAGCTG AAAGAAAAAG CCAGCACCAG CTCGAGAATT 481 TGGAACGAGA ACAGAGATTT TTAAAGTGGC GACTGGAACA GCTGCAGGGT CCTCAGGAGA 541 TGGAACGAAT ACGAATGGAC AGCATTGGAT CAACTATTTC TTCAGATCGT TCTGATTCAG 601 AGCGAGAGGA GATTGAAGTG GATGTTGAAA GCACAGAGTT CTCCCATGGA GAAGTGGACA 661 ATATAAGTAC CACCAGCATC AGTGACATTG ATGACCACAG CAGCCTGCCG AGTATTGGGA 721 GTGACGAGGG TTACTCCAGT GCCAGTGTCA AACTTTCATT CACTTCATAC CCAACTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGACAACTGA TAGAAATCAA TCTATCAACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt