Construct: ORF TRCN0000491615

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020490.2_s317c1
DNA Barcode:
ATGCTCTTGGTCATTGGCCTATCC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
EPHA2 (1969)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491615
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1969 EPHA2 EPH receptor A2 NM_004431.5 99.9% 99.8% 2876C>T
2 human 1969 EPHA2 EPH receptor A2 NM_001329090.2 94.4% 94.3% 0_1ins162;2714C>T
3 human 1969 EPHA2 EPH receptor A2 XM_017000537.1 58.3% 57.5% (many diffs)
4 mouse 13836 Epha2 Eph receptor A2 NM_010139.3 86.5% 92.6% (many diffs)
5 mouse 13836 Epha2 Eph receptor A2 XM_006538528.3 62.6% 67.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3000
ORF length:
2928
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggagctc caggcagccc gcgcctgctt cgccctgctg tggggctgtg
121cgctggccgc ggccgcggcg gcgcagggca aggaagtggt actgctggac tttgctgcag
181ctggagggga gctcggctgg ctcacacacc cgtatggcaa agggtgggac ctgatgcaga
241acatcatgaa tgacatgccg atctacatgt actccgtgtg caacgtgatg tctggcgacc
301aggacaactg gctccgcacc aactgggtgt accgaggaga ggctgagcgt atcttcattg
361agctcaagtt tactgtacgt gactgcaaca gcttccctgg tggcgccagc tcctgcaagg
421agactttcaa cctctactat gccgagtcgg acctggacta cggcaccaac ttccagaagc
481gcctgttcac caagattgac accattgcgc ccgatgagat caccgtcagc agcgacttcg
541aggcacgcca cgtgaagctg aacgtggagg agcgctccgt ggggccgctc acccgcaaag
601gcttctacct ggccttccag gatatcggtg cctgtgtggc gctgctctcc gtccgtgtct
661actacaagaa gtgccccgag ctgctgcagg gcctggccca cttccctgag accatcgccg
721gctctgatgc accttccctg gccactgtgg ccggcacctg tgtggaccat gccgtggtgc
781caccgggggg tgaagagccc cgtatgcact gtgcagtgga tggcgagtgg ctggtgccca
841ttgggcagtg cctgtgccag gcaggctacg agaaggtgga ggatgcctgc caggcctgct
901cgcctggatt ttttaagttt gaggcatctg agagcccctg cttggagtgc cctgagcaca
961cgctgccatc ccctgagggt gccacctcct gcgagtgtga ggaaggcttc ttccgggcac
1021ctcaggaccc agcgtcgatg ccttgcacac gacccccctc cgccccacac tacctcacag
1081ccgtgggcat gggtgccaag gtggagctgc gctggacgcc ccctcaggac agcgggggcc
1141gcgaggacat tgtctacagc gtcacctgcg aacagtgctg gcccgagtct ggggaatgcg
1201ggccgtgtga ggccagtgtg cgctactcgg agcctcctca cggactgacc cgcaccagtg
1261tgacagtgag cgacctggag ccccacatga actacacctt caccgtggag gcccgcaatg
1321gcgtctcagg cctggtaacc agccgcagct tccgtactgc cagtgtcagc atcaaccaga
1381cagagccccc caaggtgagg ctggagggcc gcagcaccac ctcgcttagc gtctcctgga
1441gcatcccccc gccgcagcag agccgagtgt ggaagtacga ggtcacttac cgcaagaagg
1501gagactccaa cagctacaat gtgcgccgca ccgagggttt ctccgtgacc ctggacgacc
1561tggccccaga caccacctac ctggtccagg tgcaggcact gacgcaggag ggccaggggg
1621ccggcagcaa ggtgcacgaa ttccagacgc tgtccccgga gggatctggc aacttggcgg
1681tgattggcgg cgtggctgtc ggtgtggtcc tgcttctggt gctggcagga gttggcttct
1741ttatccaccg caggaggaag aaccagcgtg cccgccagtc cccggaggac gtttacttct
1801ccaagtcaga acaactgaag cccctgaaga catacgtgga cccccacaca tatgaggacc
1861ccaaccaggc tgtgttgaag ttcactaccg agatccatcc atcctgtgtc actcggcaga
1921aggtgatcgg agcaggagag tttggggagg tgtacaaggg catgctgaag acatcctcgg
1981ggaagaagga ggtgccggtg gccatcaaga cgctgaaagc cggctacaca gagaagcagc
2041gagtggactt cctcggcgag gccggcatca tgggccagtt cagccaccac aacatcatcc
2101gcctagaggg cgtcatctcc aaatacaagc ccatgatgat catcactgag tacatggaga
2161atggggccct ggacaagttc cttcgggaga aggatggcga gttcagcgtg ctgcagctgg
2221tgggcatgct gcggggcatc gcagctggca tgaagtacct ggccaacatg aactatgtgc
2281accgtgacct ggctgcccgc aacatcctcg tcaacagcaa cctggtctgc aaggtgtctg
2341actttggcct gtcccgcgtg ctggaggacg accccgaggc cacctacacc accagtggcg
2401gcaagatccc catccgctgg accgccccgg aggccatttc ctaccggaag ttcacctctg
2461ccagcgacgt gtggagcttt ggcattgtca tgtgggaggt gatgacctat ggcgagcggc
2521cctactggga gttgtccaac cacgaggtga tgaaagccat caatgatggc ttccggctcc
2581ccacacccat ggactgcccc TCCGCCATCT ACCAGCTCAT GATGCAGTGC TGGCAGCAGG
2641AGCGTGCCCG CCGCCCCAAG TTCGCTGACA TCGTCAGCAT CCTGGACAAG CTCATTCGTG
2701CCCCTGACTC CCTCAAGACC CTGGCTGACT TTGACCCCCG CGTGTCTATC CGGCTCCCCA
2761GCACGAGCGG CTCGGAGGGG GTGCCCTTCC GCACGGTGTC CGAGTGGCTG GAGTCCATCA
2821AGATGCAGCA GTATACGGAG CACTTCATGG CGGCCGGCTA CACTGCCATC GAGAAGGTGG
2881TGCAGATGAC CAACGACGAC ATCAAGAGGA TTGGGGTGCG GCTGCCCGGC CACCAGAAGC
2941GCATCGTCTA CAGCCTGCTG GGACTCAAGG ACCAGGTGAA CACTGTGGGG ATCCCCATCT
3001AGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
3061TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
3121GGCTTTATAT ATCTTGTGGA AAGGACGAAT GCTCTTGGTC ATTGGCCTAT CCACGCGTTA
3181AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)