Construct: ORF TRCN0000491659

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013241.3_s317c1
Derived from:
ccsbBroadEn_03553
DNA Barcode:
GAAGTCACCAAGTGTTCGATTCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ETNK2 (55224)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491659
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55224 ETNK2 ethanolamine kinase 2 NM_018208.4 100% 100%
2 human 55224 ETNK2 ethanolamine kinase 2 NM_001297762.2 89.3% 89.3% 515_516ins123
3 human 55224 ETNK2 ethanolamine kinase 2 NM_001297760.2 85.1% 86.3% 1014_1119del;1182_1183ins82
4 human 55224 ETNK2 ethanolamine kinase 2 XM_024448127.1 60.4% 60.6% 0_1ins312;702_807del;870_871ins82
5 human 55224 ETNK2 ethanolamine kinase 2 NM_001297761.2 53.8% 53.8% 0_1ins534
6 human 55224 ETNK2 ethanolamine kinase 2 XM_011509714.2 42.8% 42.3% 0_1ins534;480_585del;648_649ins82
7 human 55224 ETNK2 ethanolamine kinase 2 XM_011509715.2 42.8% 42.3% 0_1ins534;480_585del;648_649ins82
8 human 55224 ETNK2 ethanolamine kinase 2 XM_017001632.1 42.8% 42.3% 0_1ins534;480_585del;648_649ins82
9 mouse 214253 Etnk2 ethanolamine kinase 2 NM_175443.5 86.4% 87.3% (many diffs)
10 mouse 214253 Etnk2 ethanolamine kinase 2 XM_006529364.2 80% 80.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1224
ORF length:
1158
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tgtgccccct tcggcccctc agccgcgcgc gtcctttcac ctgaggaggc
121acacgccttg cccgcagtgc tcatggggca tggaggagaa ggcggcggcc agcgccagct
181gccgggagcc gccgggcccc ccgagggccg ccgccgtcgc gtacttcggc atttccgtgg
241acccggacga catccttccc ggggccctgc gcctcatcca ggagctgcgg ccgcattgga
301aacccgagca agttcggacc aagcgcttca cggatggcat caccaacaag ctggtggcct
361gctatgtgga ggaggacatg caggactgcg tgctggtccg ggtgtatggg gagcggacgg
421agctgctggt ggaccgggag aatgaggtca gaaacttcca gctgctgcga gcacacagct
481gtgcccccaa actctactgc accttccaga atgggctgtg ctatgagtac atgcagggtg
541tggccctgga gcctgagcac atccgtgagc cccggctttt caggttaatc gccttagaaa
601tggcaaagat tcatactatc cacgccaacg gcagcctgcc caagcccatc ctctggcaca
661agatgcacaa ttatttcacg cttgtgaaga acgagatcaa ccccagcctt tctgcagatg
721tccctaaggt agaggtgttg gaacgggagc tggcctggct gaaggagcat ctgtcccagc
781tggagtcccc tgtggtgttt tgtcacaatg acctgctctg caagaatatc atcTATGACA
841GCATCAAAGG TCACGTGCGG TTCATTGACT ATGAATATGC TGGCTACAAC TACCAAGCTT
901TTGACATTGG CAACCATTTC AATGAGTTTG CAGGCGTGAA TGAGGTGGAT TACTGCCTGT
961ACCCGGCGCG GGAGACCCAG CTGCAGTGGC TGCACTACTA CCTGCAGGCA CAAAAGGGGA
1021TGGCCGTGAC CCCCAGGGAG GTGCAAAGGC TCTACGTGCA AGTCAACAAG TTTGCCCTGG
1081CGTCTCACTT CTTCTGGGCT CTCTGGGCCC TCATCCAGAA CCAGTACTCC ACCATCGACT
1141TTGATTTCCT CAGGTACGCA GTGATCCGAT TCAACCAGTA CTTCAAGGTG AAGCCTCAAG
1201CGTCAGCCTT GGAGATGCCA AAGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1261AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1321TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG AAGTCACCAA
1381GTGTTCGATT CTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1441att

Download FASTA (ORF) (Full)