Construct: ORF TRCN0000491664
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016051.1_s317c1
- Derived from:
- ccsbBroadEn_08248
- DNA Barcode:
- GTCGCTCCCCGTTACGTCTCCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IPO11 (51194)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491664
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51194 | IPO11 | importin 11 | NM_016338.4 | 99.9% | 100% | 783G>T |
| 2 | human | 51194 | IPO11 | importin 11 | NM_001134779.1 | 96% | 96% | 1_120del;903G>T |
| 3 | mouse | 76582 | Ipo11 | importin 11 | NM_029665.3 | 90.4% | 96.8% | (many diffs) |
| 4 | mouse | 76582 | Ipo11 | importin 11 | XM_017315635.1 | 90.3% | 96.6% | (many diffs) |
| 5 | mouse | 76582 | Ipo11 | importin 11 | XM_006517786.3 | 89.6% | 95.9% | (many diffs) |
| 6 | mouse | 76582 | Ipo11 | importin 11 | XM_006517787.2 | 89.5% | 95.7% | (many diffs) |
| 7 | mouse | 76582 | Ipo11 | importin 11 | XM_011244707.2 | 82.4% | 84.1% | (many diffs) |
| 8 | mouse | 76582 | Ipo11 | importin 11 | XM_006517788.3 | 79.5% | 84.7% | (many diffs) |
| 9 | mouse | 76582 | Ipo11 | importin 11 | XR_382746.2 | 72.4% | (many diffs) | |
| 10 | mouse | 76582 | Ipo11 | importin 11 | XM_011244708.2 | 63.5% | 67.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2991
- ORF length:
- 2925
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tctcaatagt gccagcactg ttgttcttca ggtgttaaca caggccacca 121 gtcaggatac tgctgtgtta aaaccagctg aggagcagtt gaagcagtgg gagacacagc 181 caggtttcta ttcagtgttg ctgaatattt tcaccaacca cactttggat ataaatgtaa 241 ggtggcttgc tgtactgtat tttaaacatg gaattgatcg ctactggaga cgtgtagcac 301 ctcatgctct ctcagaggag gagaaaacta ctctgcgtgc agggctcatc accaacttca 361 atgaaccaat aaaccagatt gcaactcaga ttgcagtgct cattgcaaaa gttgctagat 421 tggattgtcc cagacagtgg cctgaactaa ttcccactct tatagagtct gttaaagtcc 481 aggatgatct tcgacagcac agagcattac ttaccttcta tcatgttacc aagacactgg 541 catctaaacg acttgctgct gatagaaaac tattttatga tttagcttct ggaatttata 601 attttgcctg ctctctgtgg aatcaccaca cagacacatt cctgcaagaa gtttcttctg 661 gcaatgaagc tgcaattttg agttcactag aacgaacact gctatcattg aaagtgctgc 721 gtaagttaac tgttaatgga tttgtggaac ctcataagaa tatggaggtg atgggttttt 781 tacatggaat atttgaacgt ctaaaacagt ttctggaatg cagtagaagt ataggtacag 841 ataatgtttg tagagataga ctggaaaaga ccatcattct ttttactaaa gtgcttttgg 901 acttcttgga tcagcatcct ttttcattta ctcctctaat tcagagatca ctggaatttt 961 ctgtaagcta tgtttttaca gaagttggtg aaggcgttac atttgaacga ttcattgtcc 1021 aatgtatgaa tcttattaag atgattgtca aaaattatgc ttataagcca tccaaaaatt 1081 ttgaagatag cagccctgaa actcttgaag cccataagat taagatggca ttcttcacat 1141 atcctacttt gacagagata tgtagaagat tagtctctca ttatttccta ttaactgaag 1201 aagaactgac aatgtgggaa gaagacccag aaggctttac agtggaagaa acaggaggag 1261 attcttggaa atatagtttg aggccatgca ctgaagtatt atttatagat atattccatg 1321 aatataatca gactcttact cctgtacttc tagaaatgat gcaaacactt caaggaccca 1381 caaatgtgga agatatgaat gcactgttaa tcaaagatgc tgtgtataat gctgttggat 1441 tagctgctta tgagctcttt gacagtgttg attttgatca gtggtttaaa aaccagcttc 1501 ttccagaatt acaagtcatt cacaataggt ataagccatt gcgacgcagg gtgatttggc 1561 tcatcggtca gtggatttct gtgaaattca agtctgactt aagacccatg ctttatgaag 1621 caatctgtaa cttgcttcaa gatcaagatt tagtggtccg tattgaaaca gctacaactt 1681 tgaagttaac tgttgatgat tttgaattta gaacagatca gtttctaccg tatttggaaa 1741 ccatgttcac actacttttt cagttactgc agcaagttac agaatgtgac acaaagatgc 1801 atgttttgca tgtcctttct tgtgtgatcg aaagagtcaa catgcagata cgaccatatg 1861 tgggatgttt ggtacaatat ttgcccctcc tttggaagca gagtgaagaa cacaatatgt 1921 tgagatgtgc tattttgaca acacttattc atcttgttca gggattagga gcagacagca 1981 agaacctgta ccctttcctg ctcccagtta ttcaactgag tacagatgtt tcacagcctc 2041 cacatgttta tcttctggaa gatggtttag aattatggtt agtaactttg gaaaacagtc 2101 catgtattac accagagttg cttcgtatat ttcagaatat gtcaccactt cttgaactaa 2161 gttcagaaaa tcttagaact tgctttaaga tcatcaatgg ttatatcttt ttatcatcaa 2221 cagaattttt acagacatac gcagtaggtc tatgccagtc cttttgtgaa cttttaaagg 2281 aaattactac agaaggtcaa gttcaggtgc tcaaggttgt ggaaaatgcc cttaaagtga 2341 acccaatact aggtccacaa atgtttcaac cgattttacc ctatgttttc aagggtatta 2401 tagaagggga gaggtatcct gtagtgatgt ccacgtatct tggagttatg ggtcgagttc 2461 tactacaaaa cactagtttt ttttcttcac tacttaatga gatggcccat aaatttaatc 2521 aggagatgga ccagcttttg ggaaatatga ttgaaatgtg ggttgatcga atggacaaca 2581 ttacccagcc tgaaagaaga aaactttcag ctttggcttt gctctctctt ctgccatctg 2641 ataatagtgt tatccaagat aaattctgtg ggattataaa catttcagta gaaggcctgc 2701 atgatgtcat gacggaagat cctgaaacag gaacttataa agactgtatg ttgatgtctc 2761 atcttgagga accaaaagta acagaagatg aagaaccacC CACAGAACAA GATAAGAGGA 2821 AAAAGATGCT GGCCCTGAAG GACCCTGTTC ATACAGTGTC ACTGCAGCAG TTCATCTACG 2881 AGAAGCTCAA GGCACAGCAG GAGATGCTAG GAGAACAAGG TTTCCAGTCC CTCATGGAAA 2941 CAGTGGATAC GGAGATTGTC ACCCAGCTAC AGGAGTTTTT GCAAGGATTC TACCCAACTT 3001 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 3061 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 3121 CTTGTGGAAA GGACGAGTCG CTCCCCGTTA CGTCTCCTAC ACGCGTTAAG TCgacaatca 3181 acctctggat tacaaaattt gtgaaagatt