Construct: ORF TRCN0000491666
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019839.2_s317c1
- DNA Barcode:
- ACGCCATGAGATTTACAGCCTAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALPK1 (80216)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491666
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80216 | ALPK1 | alpha kinase 1 | NM_001102406.2 | 99.9% | 99.9% | 318T>C;3732_3733insG |
| 2 | human | 80216 | ALPK1 | alpha kinase 1 | NM_025144.4 | 99.9% | 99.9% | 318T>C;3732_3733insG |
| 3 | human | 80216 | ALPK1 | alpha kinase 1 | NM_001253884.1 | 93.6% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3804
- ORF length:
- 3735
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaataatcaa aaagtggtag ctgtgctact gcaagagtgc aagcaagtgc 121 tggatcagct cttgttggaa gcgccagatg tgtcggaaga ggacaagagc gaggaccagc 181 gctgcagagc tttactcccc agcgagttaa ggaccctgat ccaggaggca aaggaaatga 241 agtggccctt cgtgcctgaa aagtggcagt acaaacaagc cgtgggccca gaggacaaaa 301 caaacctgaa ggatgtgatt ggcgccgggt tgcagcagtt actggcgtcc ctgagggcct 361 ccatcctcgc tcgggactgt gcggccgcgg cggctattgt gttcttggtg gaccggttcc 421 tgtatgggct cgacgtctct ggaaaacttc tgcaggtcgc caaaggtctc cacaagttgc 481 agccagccac gccaattgcc ccgcaggtgg ttattcgcca agcccgaatc tccgtgaact 541 caggaaaact tttaaaagca gagtatattc tgagcagtct aataagcaac aatggagcaa 601 cgggtacctg gctgtacaga aatgaaagtg acaaggtcct ggtgcagtcg gtctgtatac 661 agatcagagg gcagattctg caaaagctgg ggatgtggta cgaagcagca gagttaatat 721 gggcctccat tgtaggatat ttggcacttc ctcagccgga taaaaagggc ctctccacgt 781 cgctaggtat actggcagac atctttgttt ccatgagcaa gaacgattat gaaaagttta 841 aaaacaatcc acaaattaat ttgagcctgc tgaaggagtt tgaccaccat ttgctgtccg 901 ctgcagaagc ctgcaagctg gcagctgcct tcagtgccta tacgccgctc ttcgtgctca 961 cagctgtgaa tatccgtggc acgtgtttat tgtcctacag tagttcaaat gactgtcctc 1021 cagaattgaa aaacttacat ctgtgtgaag ccaaagaggc ctttgagatt ggcctcctca 1081 ccaagagaga tgatgagcct gttactggaa aacaggagct tcacagcttt gtcaaagctg 1141 ctttcggtct caccacagtg cacagaaggc tccatgggga gacagggacg gtccatgcag 1201 caagtcagct ctgtaaggaa gcaatgggga agctgtacaa tttcagcact tcctccagaa 1261 gtcaggacag agaagctctg tctcaagaag ttatgtctgt gattgcccag gtgaaggaac 1321 atttacaagt tcaaagcttc tcaaatgtag atgacagatc ttatgttccc gagagtttcg 1381 agtgcaggtt ggataaactt atcttgcatg ggcaagggga tttccaaaaa atccttgaca 1441 cctattcaca gcaccatact tcggtgtgtg aagtatttga aagtgattgt ggaaacaaca 1501 aaaatgaaca gaaagatgca aaaacaggag tctgcatcac tgctctaaaa acagaaataa 1561 aaaacataga tactgtgagt actactcaag aaaagccaca ttgtcaaaga gacacaggaa 1621 tatcttcctc cctaatgggt aagaatgttc agagggaact cagaagggga ggaaggagaa 1681 actggaccca ttctgatgca tttcgagtct ccttggatca agatgtggag actgagactg 1741 agccatcgga ctacagcaat ggtgagggag ctgttttcaa caagtctctg agtggcagcc 1801 agacttccag tgcttggagc aacttatcag ggtttagttc ctctgcaagc tgggaggaag 1861 tgaattatca cgttgacgac aggtcagcca gaaaagagcc tggcaaagaa catctggtgg 1921 acactcagtg ttccactgcc ttgtctgagg agctagagaa tgacagggaa ggcagagcta 1981 tgcattcatt gcattcacag cttcatgatc tctctcttca ggaacccaac aatgacaatt 2041 tggagccttc tcaaaatcag ccacagcaac agatgccctt gacacccttc tcgcctcata 2101 ataccccagg cattttcttg gcccctggtg cagggcttct agaaggagct ccagaaggta 2161 tccaggaagt cagaaatatg ggacccagaa atacttctgc tcactccaga ccctcatatc 2221 gttctgcttc ttggtcttct gattctggta ggcccaagaa tatgggcaca catccttcag 2281 tccaaaaaga agaagccttt gaaataattg ttgagtttcc agaaaccaac tgcgatgtca 2341 aagacaggca ggggaaagag cagggagaag aaattagtga aagaggcgca ggccctacat 2401 ttaaagctag tccctcctgg gttgacccag aaggagaaac agcagaaagc actgaagatg 2461 cacccttaga ctttcacagg gtcctgcaca attctctggg aaacatttcc atgctgccat 2521 gtagctcctt cacccctaat tggcctgttc aaaatcctga ctccagaaaa agtggtggcc 2581 cagtcgcaga gcagggcatc gaccctgatg cctccacagt ggatgaggag gggcaactgc 2641 tcgacagcat ggatgttccc tgcacaaatg ggcacggctc tcatagactg tgcattctga 2701 gacagccgcc tggtcagagg gcggagaccc ccaattcctc tgtaagcggt aacatcctct 2761 tccctgtcct cagcgaggac tgcactacca cagaggaagg aaatcagcct ggaaacatgc 2821 taaactgcag ccagaactcc agctcatcct cagtgtggtg gctgaaatca cctgcatttt 2881 ccagtggttc ttctgagggg gacagccctt ggtcctatct gaattccagt gggagttctt 2941 gggtttcatt gccgggaaag atgaggaaag agatccttga ggctcgcacc ttgcaacctg 3001 atgactttga aaagctgttg gcaggagtga ggcatgattg gctgtttcag agactagaga 3061 atacgggggt ttttaagccc agtcaactcc accgagcaca tagtgctctt ttgttaaaat 3121 attcaaaaaa atctgaactg tggacggccc aggaaactat tgtctatttg ggggactact 3181 tgactgtgaa gaaaaaaggc agacaaagaa atgctttttg ggttcatcat cttcatcaag 3241 aagaaattct ggggaggtat gttgggaaag actataagga gcagaagggg ctctggcacc 3301 acttcactga tgtggagcga cagatgaccg cacagcacta tgtgacagaa tttaacaaga 3361 gactctatga acaaaacatt ccCACCCAGA TATTCTACAT CCCATCCACA ATACTACTGA 3421 TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA CTGGGAGAAT 3481 TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC ACAGAATATG 3541 GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT GTGGTCGATT 3601 TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT CCCCAGATTC 3661 ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT TTTTACTTCT 3721 TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG ACTAGACCTT 3781 CAATGGAGAA ACCATGCACA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3841 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3901 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACGC CATGAGATTT 3961 ACAGCCTAGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt