Construct: ORF TRCN0000491668
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019692.2_s317c1
- DNA Barcode:
- CAGCGTATAACCGGCGGCCTCGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMX (660)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491668
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 660 | BMX | BMX non-receptor tyrosine k... | NM_001721.7 | 99.9% | 99.8% | 2025_2026insG |
| 2 | human | 660 | BMX | BMX non-receptor tyrosine k... | NM_203281.3 | 99.9% | 99.8% | 2025_2026insG |
| 3 | human | 660 | BMX | BMX non-receptor tyrosine k... | NM_001320866.2 | 99.8% | 99.7% | 750_751insAGT;2022_2023insG |
| 4 | human | 660 | BMX | BMX non-receptor tyrosine k... | XM_017029752.2 | 70.6% | 68.1% | 1394_1395ins217;1431_1432ins378 |
| 5 | mouse | 12169 | Bmx | BMX non-receptor tyrosine k... | NM_009759.4 | 85.2% | 86.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2103
- ORF length:
- 2028
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggat acaaaatcta ttctagaaga acttcttctc aaaagatcac 121 agcaaaagaa gaaaatgtca ccaaataatt acaaagaacg gctttttgtt ttgaccaaaa 181 caaacctttc ctactatgaa tatgacaaaa tgaaaagggg cagcagaaaa ggatccattg 241 aaattaagaa aatcagatgt gtggagaaag taaatctcga ggagcagacg cctgtagaga 301 gacagtaccc atttcagatt gtctataaag atgggcttct ctatgtctat gcatcaaatg 361 aagagagccg aagtcagtgg ttgaaagcat tacaaaaaga gataaggggt aacccccacc 421 tgctggtcaa gtaccatagt gggttcttcg tggacgggaa gttcctgtgt tgccagcaga 481 gctgtaaagc agccccagga tgtaccctct gggaagcata tgctaatctg catactgcag 541 tcaatgaaga gaaacacaga gttcccacct tcccagacag agtgctgaag atacctcggg 601 cagttcctgt tctcaaaatg gatgcaccat cttcaagtac cactctagcc caatatgaca 661 acgaatcaaa gaaaaactat ggctcccagc caccatcttc aagtaccagt ctagcgcaat 721 atgacagcaa ctcaaagaaa atctatggct cccagccaaa cttcaacatg cagtatattc 781 caagggaaga cttccctgac tggtggcaag taagaaaact gaaaagtagc agcagcagtg 841 aagatgttgc aagcagtaac caaaaagaaa gaaatgtgaa tcacaccacc tcaaagattt 901 catgggaatt ccctgagtca agttcatctg aagaagagga aaacctggat gattatgact 961 ggtttgctgg taacatctcc agatcacaat ctgaacagtt actcagacaa aagggaaaag 1021 aaggagcatt tatggttaga aattcgagcc aagtgggaat gtacacagtg tccttattta 1081 gtaaggctgt gaatgataaa aaaggaactg tcaaacatta ccacgtgcat acaaatgctg 1141 agaacaaatt atacctggca gaaaactact gttttgattc cattccaaag cttattcatt 1201 atcatcaaca caattcagca ggcatgatca cacggctccg ccaccctgtg tcaacaaagg 1261 ccaacaaggt ccccgactct gtgtccctgg gaaatggaat ctgggaactg aaaagagaag 1321 agattacctt gttgaaggag ctgggaagtg gccagtttgg agtggtccag ctgggcaagt 1381 ggaaggggca gtatgatgtt gctgttaaga tgatcaagga gggctccatg tcagaagatg 1441 aattctttca ggaggcccag actatgatga aactcagcca tcccaagctg gttaaattct 1501 atggagtgtg ttcaaaggaa taccccatat acatagtgac tgaatatata agcaatggct 1561 gcttgctgaa ttacctgagg agtcacggaa aaggacttga accttcccag ctcttagaaa 1621 tgtgctacga tgtctgtgaa ggcatggcct tcttggagag tcaccaattc atacaccggg 1681 acttggctgc tcgtaactgc ttggtggaca gagatctctg tgtgaaagta tctgactttg 1741 gaatgacaag gtatgttctt gatgaccagt atgtcagttc agtcggaaca aagtttccag 1801 tcaagtggTC AGCTCCAGAG GTGTTTCATT ACTTCAAATA CAGCAGCAAG TCAGACGTAT 1861 GGGCATTTGG GATCCTGATG TGGGAGGTGT TCAGCCTGGG GAAGCAGCCC TATGACTTGT 1921 ATGACAACTC CCAGGTGGTT CTGAAGGTCT CCCAGGGCCA CAGGCTTTAC CGGCCCCACC 1981 TGGCATCGGA CACCATCTAC CAGATCATGT ACAGCTGCTG GCACGAGCTT CCAGAAAAGC 2041 GTCCCACATT TCAGCAACTC CTGTCTTCCA TTGAACCACT TCGGGAAAAA GACAAGCATG 2101 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2161 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2221 TTTATATATC TTGTGGAAAG GACGACAGCG TATAACCGGC GGCCTCGTAA CGCGTTAAGT 2281 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt