Construct: ORF TRCN0000491673

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020809.2_s317c1
DNA Barcode:
AACGATTATTCATTAATTTAGTAA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
CXCR5 (643)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491673
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 643 CXCR5 C-X-C motif chemokine recep... NM_032966.2 99.8% 100% 879G>C
2 human 643 CXCR5 C-X-C motif chemokine recep... NM_001716.5 87.8% 87.9% 1_135del;1014G>C
3 mouse 12145 Cxcr5 chemokine (C-X-C motif) rec... NM_007551.2 73.5% 77.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1053
ORF length:
981
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggcctcc ttcaaggccg tgttcgtgcc cgtggcctac agcctcatct
121tcctcctggg cgtgatcggc aacgtcctgg tgctggtgat cctggagcgg caccggcaga
181cacgcagttc cacggagacc ttcctgttcc acctggccgt ggccgacctc ctgctggtct
241tcatcttgcc ctttgccgtg gccgagggct ctgtgggctg ggtcctgggg accttcctct
301gcaaaactgt gattgccctg cacaaagtca acttctactg cagcagcctg ctcctggcct
361gcatcgccgt ggaccgctac ctggccattg tccacgccgt ccatgcctac cgccaccgcc
421gcctcctctc catccacatc acctgtggga ccatctggct ggtgggcttc ctccttgcct
481tgccagagat tctcttcgcc aaagtcagcc aaggccatca caacaactcc ctgccacgtt
541gcaccttctc ccaagagaac caagcagaaa cgcatgcctg gttcacctcc cgattcctct
601accatgtggc gggattccTG CTGCCCATGC TGGTGATGGG CTGGTGCTAC GTGGGGGTAG
661TGCACAGGTT GCGCCAGGCC CAGCGGCGCC CTCAGCGGCA GAAGGCAGTC AGGGTGGCCA
721TCCTGGTGAC AAGCATCTTC TTCCTCTGCT GGTCACCCTA CCACATCGTC ATCTTCCTGG
781ACACCCTGGC GAGGCTGAAG GCCGTGGACA ATACCTGCAA GCTGAATGGC TCTCTCCCCG
841TGGCCATCAC CATGTGTGAG TTCCTGGGCC TGGCCCACTG CTGCCTCAAC CCCATGCTCT
901ACACTTTCGC CGGCGTGAAG TTCCGCAGTG ACCTGTCGCG GCTCCTGACC AAGCTGGGCT
961GTACCGGCCC TGCCTCCCTG TGCCAGCTCT TCCCTAGCTG GCGCAGGAGC AGTCTCTCTG
1021AGTCAGAGAA TGCCACCTCT CTCACCACGT TCTAGGACCC AGCTTTCTTG TACAAAGTGG
1081TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1141CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1201AAACGATTAT TCATTAATTT AGTAAACGCG TTAAGTCgac aatcaacctc tggattacaa
1261aatttgtgaa agatt

Download FASTA (ORF) (Full)