Construct: ORF TRCN0000491673
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020809.2_s317c1
- DNA Barcode:
- AACGATTATTCATTAATTTAGTAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CXCR5 (643)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491673
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 643 | CXCR5 | C-X-C motif chemokine recep... | NM_032966.2 | 99.8% | 100% | 879G>C |
| 2 | human | 643 | CXCR5 | C-X-C motif chemokine recep... | NM_001716.5 | 87.8% | 87.9% | 1_135del;1014G>C |
| 3 | mouse | 12145 | Cxcr5 | chemokine (C-X-C motif) rec... | NM_007551.2 | 73.5% | 77.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1053
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcctcc ttcaaggccg tgttcgtgcc cgtggcctac agcctcatct 121 tcctcctggg cgtgatcggc aacgtcctgg tgctggtgat cctggagcgg caccggcaga 181 cacgcagttc cacggagacc ttcctgttcc acctggccgt ggccgacctc ctgctggtct 241 tcatcttgcc ctttgccgtg gccgagggct ctgtgggctg ggtcctgggg accttcctct 301 gcaaaactgt gattgccctg cacaaagtca acttctactg cagcagcctg ctcctggcct 361 gcatcgccgt ggaccgctac ctggccattg tccacgccgt ccatgcctac cgccaccgcc 421 gcctcctctc catccacatc acctgtggga ccatctggct ggtgggcttc ctccttgcct 481 tgccagagat tctcttcgcc aaagtcagcc aaggccatca caacaactcc ctgccacgtt 541 gcaccttctc ccaagagaac caagcagaaa cgcatgcctg gttcacctcc cgattcctct 601 accatgtggc gggattccTG CTGCCCATGC TGGTGATGGG CTGGTGCTAC GTGGGGGTAG 661 TGCACAGGTT GCGCCAGGCC CAGCGGCGCC CTCAGCGGCA GAAGGCAGTC AGGGTGGCCA 721 TCCTGGTGAC AAGCATCTTC TTCCTCTGCT GGTCACCCTA CCACATCGTC ATCTTCCTGG 781 ACACCCTGGC GAGGCTGAAG GCCGTGGACA ATACCTGCAA GCTGAATGGC TCTCTCCCCG 841 TGGCCATCAC CATGTGTGAG TTCCTGGGCC TGGCCCACTG CTGCCTCAAC CCCATGCTCT 901 ACACTTTCGC CGGCGTGAAG TTCCGCAGTG ACCTGTCGCG GCTCCTGACC AAGCTGGGCT 961 GTACCGGCCC TGCCTCCCTG TGCCAGCTCT TCCCTAGCTG GCGCAGGAGC AGTCTCTCTG 1021 AGTCAGAGAA TGCCACCTCT CTCACCACGT TCTAGGACCC AGCTTTCTTG TACAAAGTGG 1081 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1141 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1201 AAACGATTAT TCATTAATTT AGTAAACGCG TTAAGTCgac aatcaacctc tggattacaa 1261 aatttgtgaa agatt