Construct: ORF TRCN0000491674
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019746.2_s317c1
- DNA Barcode:
- TAACCTACCTTCTGGATACAGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABCC3 (8714)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491674
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8714 | ABCC3 | ATP binding cassette subfam... | NM_003786.4 | 99.9% | 99.9% | 4581_4582insG |
| 2 | human | 8714 | ABCC3 | ATP binding cassette subfam... | NM_001144070.2 | 36.4% | 35.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 4659
- ORF length:
- 4584
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggac gccctgtgcg gttccgggga gctcggctcc aagttctggg 121 actccaacct gtctgtgcac acagaaaacc cggacctcac tccctgcttc cagaactccc 181 tgctggcctg ggtgccctgc atctacctgt gggtcgccct gccctgctac ttgctctacc 241 tgcggcacca ttgtcgtggc tacatcatcc tctcccacct gtccaagctc aagatggtcc 301 tgggtgtcct gctgtggtgc gtctcctggg cggacctttt ttactccttc catggcctgg 361 tccatggccg ggcccctgcc cctgttttct ttgtcacccc cttggtggtg ggggtcacca 421 tgctgctggc caccctgctg atacagtatg agcggctgca gggcgtacag tcttcggggg 481 tcctcattat cttctggttc ctgtgtgtgg tctgcgccat cgtcccattc cgctccaaga 541 tccttttagc caaggcagag ggtgagatct cagacccctt ccgcttcacc accttctaca 601 tccactttgc cctggtactc tctgccctca tcttggcctg cttcagggag aaacctccat 661 ttttctccgc aaagaatgtc gaccctaacc cctaccctga gaccagcgct ggctttctct 721 cccgcctgtt tttctggtgg ttcacaaaga tggccatcta tggctaccgg catcccctgg 781 aggagaagga cctctggtcc ctaaaggaag aggacagatc ccagatggtg gtgcagcagc 841 tgctggaggc atggaggaag caggaaaagc agacggcacg acacaaggct tcagcagcac 901 ctgggaaaaa tgcctccggc gaggacgagg tgctgctggg tgcccggccc aggccccgga 961 agccctcctt cctgaaggcc ctgctggcca ccttcggctc cagcttcctc atcagtgcct 1021 gcttcaagct tatccaggac ctgctctcct tcatcaatcc acagctgctc agcatcctga 1081 tcaggtttat ctccaacccc atggccccct cctggtgggg cttcctggtg gctgggctga 1141 tgttcctgtg ctccatgatg cagtcgctga tcttacaaca ctattaccac tacatctttg 1201 tgactggggt gaagtttcgt actgggatca tgggtgtcat ctacaggaag gctctggtta 1261 tcaccaactc agtcaaacgt gcgtccactg tgggggaaat tgtcaacctc atgtcagtgg 1321 atgcccagcg cttcatggac cttgccccct tcctcaatct gctgtggtca gcacccctgc 1381 agatcatcct ggcgatctac ttcctctggc agaacctagg tccctctgtc ctggctggag 1441 tcgctttcat ggtcttgctg attccactca acggagctgt ggccgtgaag atgcgcgcct 1501 tccaggtaaa gcaaatgaaa ttgaaggact cgcgcatcaa gctgatgagt gagatcctga 1561 acggcatcaa ggtgctgaag ctgtacgcct gggagcccag cttcctgaag caggtggagg 1621 gcatcaggca gggtgagctc cagctgctgc gcacggcggc ctacctccac accacaacca 1681 ccttcacctg gatgtgcagc cccttcctgg tgaccctgat caccctctgg gtgtacgtgt 1741 acgtggaccc aaacaatgtg ctggacgccg agaaggcctt tgtgtctgtg tccttgttta 1801 atatcttaag acttcccctc aacatgctgc cccagttaat cagcaacctg actcaggcca 1861 gtgtgtctct gaaacggatc cagcaattcc tgagccaaga ggaacttgac ccccagagtg 1921 tggaaagaaa gaccatctcc ccaggctatg ccatcaccat acacagtggc accttcacct 1981 gggcccagga cctgcccccc actctgcaca gcctagacat ccaggtcccg aaaggggcac 2041 tggtggccgt ggtggggcct gtgggctgtg ggaagtcctc cctggtgtct gccctgctgg 2101 gagagatgga gaagctagaa ggcaaagtgc acatgaaggg ctccgtggcc tatgtgcccc 2161 agcaggcatg gatccagaac tgcactcttc aggaaaacgt gcttttcggc aaagccctga 2221 accccaagcg ctaccagcag actctggagg cctgtgcctt gctagctgac ctggagatgc 2281 tgcctggtgg ggatcagaca gagattggag agaagggcat taacctgtct gggggccagc 2341 ggcagcgggt cagtctggct cgagctgttt acagtgatgc cgatattttc ttgctggatg 2401 acccactgtc cgcggtggac tctcatgtgg ccaagcacat ctttgaccac gtcatcgggc 2461 cagaaggcgt gctggcaggc aagacgcgag tgctggtgac gcacggcatt agcttcctgc 2521 cccagacaga cttcatcatt gtgctagctg atggacaggt gtctgagatg ggcccgtacc 2581 cagccctgct gcagcgcaac ggctcctttg ccaactttct ctgcaactat gcccccgatg 2641 aggaccaagg gcacctggag gacagctgga ccgcgttgga aggtgcagag gataaggagg 2701 cactgctgat tgaagacaca ctcagcaacc acacggatct gacagacaat gatccagtca 2761 cctatgtggt ccagaagcag tttatgagac agctgagtgc cctgtcctca gatggggagg 2821 gacagggtcg gcctgtaccc cggaggcacc tgggtccatc agagaaggtg caggtgacag 2881 aggcgaaggc agatggggca ctgacccagg aggagaaagc agccattggc actgtggagc 2941 tcagtgtgtt ctgggattat gccaaggccg tggggctctg taccacgctg gccatctgtc 3001 tcctgtatgt gggtcaaagt gcggctgcca ttggagccaa tgtgtggctc agtgcctgga 3061 caaatgatgc catggcagac agtagacaga acaacacttc cctgaggctg ggcgtctatg 3121 ctgctttagg aattctgcaa gggttcttgg tgatgctggc agccatggcc atggcagcgg 3181 gtggcatcca ggctgcccgt gtgttgcacc aggcactgct gcacaacaag atacgctcgc 3241 cacagtcctt ctttgacacc acaccatcag gccgcatcct gaactgcttc tccaaggaca 3301 tctatgtcgt tgatgaggtt ctggcccctg tcatcctcat gctgctcaat tccttcttca 3361 acgccatctc cactcttgtg gtcatcatgg ccagcacgcc gctcttcact gtggtcatcc 3421 tgcccctggc tgtgctctac accttagtgc agcgcttcta tgcagccaca tcacggcaac 3481 tgaagcggct ggaatcagtc agccgctcac ctatctactc ccacttttcg gagacagtga 3541 ctggtgccag tgtcatccgg gcctacaacc gcagccggga ttttgagatc atcagtgata 3601 ctaaggtgga tgccaaccag agaagctgct acccctacat catctccaac cggtggctga 3661 gcatcggagt ggagttcgtg gggaactgcg tggtgctctt tgctgcacta tttgccgtca 3721 tcgggaggag cagcctgaac ccggggctgg tgggcctttc tgtgtcctac tccttgcagg 3781 tgacatttgc tctgaactgg atgatacgaa tgatgtcaga tttggaatct aacatcgtgg 3841 ctgtggagag ggtcaaggag tactccaaga cagagacaga ggcgccctgg gtggtggaag 3901 gcagccgccc tcccgaaggt tggcccccac gtggggaggt ggagttccgg aattattctg 3961 tgcgctaccg gccgggccta gacctggtgc tgagagacct gagtctgcat gtgcacggtg 4021 gcgagaaggt ggggatcgtg ggccgcactg gggctggcaa gtcttccatg accctttgcc 4081 tgttccgcat cctggaggcg gcaaagggtg aaatccgcat tgatggcctc aatgtggcag 4141 acatcggcct ccatgacctg cgctctcagc tgaccatcat cccgcaggac cccatcctgt 4201 tctcggggac cctgcgcatg aacctggacc ccttcggcag ctactcagag gaggacattt 4261 ggtgggcttt ggagctgtcc cacctgcaca cgtttgtgag ctcccagccg gcaggcctgg 4321 acttccagtg ctcagagggc ggggagaatc TCAGCGTGGG CCAGAGGCAG CTCGTGTGCC 4381 TGGCCCGAGC CCTGCTCCGC AAGAGCCGCA TCCTGGTTTT AGACGAGGCC ACAGCTGCCA 4441 TCGACCTGGA GACTGACAAC CTCATCCAGG CTACCATCCG CACCCAGTTT GATACCTGCA 4501 CTGTCCTGAC CATCGCACAC CGGCTTAACA CTATCATGGA CTACACCAGG GTCCTGGTCC 4561 TGGACAAAGG AGTAGTAGCT GAATTTGATT CTCCAGCCAA CCTCATTGCA GCTAGAGGCA 4621 TCTTCTACGG GATGGCCAGA GATGCTGGAC TTGCCGACCC AGCTTTCTTG TACAAAGTGG 4681 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 4741 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 4801 ATAACCTACC TTCTGGATAC AGATCACGCG TTAAGTCgac aatcaacctc tggattacaa 4861 aatttgtgaa agatt