Construct: ORF TRCN0000491691

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019731.2_s317c1
DNA Barcode:
CTCGCGTGTAATCTAAGGTGCTGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MKNK2 (2872)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491691
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2872 MKNK2 MAPK interacting serine/thr... NM_199054.2 99.9% 99.7% 1395_1396insG
2 human 2872 MKNK2 MAPK interacting serine/thr... NM_017572.4 87.1% 81.3% (many diffs)
3 human 2872 MKNK2 MAPK interacting serine/thr... XM_024451471.1 79.4% 74.2% (many diffs)
4 mouse 17347 Mknk2 MAP kinase-interacting seri... NM_021462.4 87.4% 92.4% (many diffs)
5 mouse 17347 Mknk2 MAP kinase-interacting seri... XM_006513316.1 87.4% 92.4% (many diffs)
6 mouse 17347 Mknk2 MAP kinase-interacting seri... XM_017313824.1 78.7% 84.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1470
ORF length:
1398
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggtgcag aagaaaccag ccgaacttca gggtttccac cgttcgttca
121aggggcagaa ccccttcgag ctggccttct ccctagacca gcccgaccac ggagactctg
181actttggcct gcagtgctca gcccgccctg acatgcccgc cagccagccc attgacatcc
241cggacgccaa gaagaggggc aagaagaaga agcgcggccg ggccaccgac agcttctcgg
301gcaggtttga agacgtctac cagctgcagg aagatgtgct gggggagggc gctcatgccc
361gagtgcagac ctgcatcaac ctgatcacca gccaggagta cgccgtcaag atcattgaga
421agcagccagg ccacattcgg agcagggttt tcagggaggt ggagatgctg taccagtgcc
481agggacacag gaacgtccta gagctgattg agttcttcga ggaggaggac cgcttctacc
541tggtgtttga gaagatgcgg ggaggctcca tcctgagcca catccacaag cgccggcact
601tcaacgagct ggaggccagc gtggtggtgc aggacgtggc cagcgccttg gactttctgc
661ataacaaagg catcgcccac agggacctaa agccggaaaa catcctctgt gagcacccca
721accaggtctc ccccgtgaag atctgtgact tcgacctggg cagcggcatc aaactcaacg
781gggactgctc ccctatctcc accccggagc tgctcactcc gtgcggctcg gcggagtaca
841tggccccgga ggtagtggag gccttcagcg aggaggctag catctacgac aagcgctgcg
901acctgtggag cctgggcgtc atcttgtata tcctactcag cggctacccg cccttcgtgg
961gccgctgtgg cagcgactgc ggctgggacc gcggcgaggc ctgccctgcc tgccagaaca
1021tgctgtttga gagcatccag gagggcaagt acgagttccc cgacaaggac tgggcccaca
1081tctcctgcgc tgccaaagac cTCATCTCCA AGCTGCTGGT CCGTGACGCC AAGCAGAGGC
1141TGAGTGCCGC CCAAGTCCTG CAGCACCCCT GGGTTCAGGG GTGCGCCCCG GAGAACACCT
1201TGCCCACTCC CATGGTCCTG CAGAGGAACA GCTGTGCCAA AGACCTCACG TCCTTCGCGG
1261CTGAGGCCAT TGCCATGAAC CGGCAGCTGG CCCAGCACGA CGAGGACCTG GCTGAGGAGG
1321AGGCCGCGGG GCAGGGCCAG CCCGTCCTGG TCCGAGCTAC CTCACGCTGC CTGCAGCTGT
1381CTCCACCCTC CCAGTCCAAG CTGGCGCAGC GGCGGCAAAG GGCCAGTCTG TCCTCGGCCC
1441CAGTGGTCCT GGTGGGAGAC CACGCCGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
1501GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1561AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTCGCGTG
1621TAATCTAAGG TGCTGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1681aagatt

Download FASTA (ORF) (Full)

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