Construct: ORF TRCN0000491693
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021352.2_s317c1
- DNA Barcode:
- CTTAATGCTCTTTTTAATCCTTGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DUSP22 (56940)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491693
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56940 | DUSP22 | dual specificity phosphatas... | NM_020185.4 | 99.6% | 100% | 12G>C;516G>A |
| 2 | human | 56940 | DUSP22 | dual specificity phosphatas... | NM_001286555.1 | 85.7% | 83.9% | (many diffs) |
| 3 | human | 56940 | DUSP22 | dual specificity phosphatas... | XM_017011062.1 | 66.6% | 65.7% | (many diffs) |
| 4 | human | 56940 | DUSP22 | dual specificity phosphatas... | XM_011514757.2 | 56.5% | 53.3% | (many diffs) |
| 5 | human | 56940 | DUSP22 | dual specificity phosphatas... | NR_104473.1 | 13.7% | (many diffs) | |
| 6 | human | 56940 | DUSP22 | dual specificity phosphatas... | NR_104475.1 | 13.6% | (many diffs) | |
| 7 | human | 56940 | DUSP22 | dual specificity phosphatas... | NR_104474.1 | 12.7% | (many diffs) | |
| 8 | mouse | 105352 | Dusp22 | dual specificity phosphatas... | NM_134068.3 | 88.9% | 93.4% | (many diffs) |
| 9 | mouse | 105352 | Dusp22 | dual specificity phosphatas... | NM_001037955.4 | 76.3% | 76.6% | (many diffs) |
| 10 | mouse | 105352 | Dusp22 | dual specificity phosphatas... | XM_006516519.1 | 74.7% | 74.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 624
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggggaat ggcatgaaca agatcctgcc cggcctgtac atcggcaact 121 tcaaagatgc cagagacgcg gaacaattga gcaagaacaa ggtgacacat attctgtctg 181 tccacgatag tgccaggcct atgttggagg gagttaaata cctgtgcatc ccagcagcgg 241 attcaccatc tcaaaacctg acaagacatt tcaaagaaag tattaaattc attcacgagt 301 gccggcTCCG CGGTGAGAGC TGCCTTGTAC ACTGCCTGGC CGGGGTCTCC AGGAGCGTGA 361 CACTGGTGAT CGCATACATC ATGACCGTCA CTGACTTTGG CTGGGAGGAT GCCCTGCACA 421 CCGTGCGTGC TGGGAGATCC TGTGCCAACC CCAACGTGGG CTTCCAGAGA CAGCTCCAGG 481 AGTTTGAGAA GCATGAGGTC CATCAGTATC GGCAGTGGCT GAAGGAAGAA TATGGAGAGA 541 GCCCTTTGCA GGATGCAGAA GAAGCCAAAA ACATTCTGGC CGCTCCAGGA ATTCTGAAGT 601 TCTGGGCCTT TCTCAGAAGA CTGTAGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 661 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 721 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTTAATGC 781 TCTTTTTAAT CCTTGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 841 aagatt