Construct: ORF TRCN0000491712
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005600.1_s317c1
- Derived from:
- ccsbBroadEn_15517
- DNA Barcode:
- GCGTGGTCCATTCAGGTCCCAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P4HB (5034)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491712
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5034 | P4HB | prolyl 4-hydroxylase subuni... | NM_000918.4 | 36.2% | 36.2% | 1_969del;1012A>T;1365T>C |
| 2 | human | 5034 | P4HB | prolyl 4-hydroxylase subuni... | XM_024450777.1 | 32.6% | 14.2% | (many diffs) |
| 3 | human | 5034 | P4HB | prolyl 4-hydroxylase subuni... | XR_002958014.1 | 19.9% | (many diffs) | |
| 4 | mouse | 18453 | P4hb | prolyl 4-hydroxylase, beta ... | NM_011032.2 | 31.1% | 34.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 621
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac caagtacaag cccgaatcgg aggagctgac ggcagagtgg atcacagagt 121 tctgccaccg cttcctggag ggcaaaatca agccccacct gatgagccag gagctgccgg 181 aggactggga caagcagcct gtcaaggtgc ttgttgggaa gaactttgaa gacgtggctt 241 ttgatgagaa aaaaaacgtc ttTGTGGAGT TCTATGCCCC ATGGTGTGGT CACTGCAAAC 301 AGTTGGCTCC CATTTGGGAT AAACTGGGAG AGACGTACAA GGACCATGAG AACATCGTCA 361 TCGCCAAGAT GGACTCGACT GCCAACGAGG TGGAGGCCGT CAAAGTGCAC AGCTTCCCCA 421 CACTCAAGTT CTTTCCTGCC AGTGCCGACA GGACGGTCAT CGATTACAAC GGGGAACGCA 481 CGCTGGATGG TTTTAAGAAA TTCCTGGAGA GCGGTGGCCA GGATGGGGCA GGGGATGATG 541 ACGATCTCGA GGACCTGGAA GAAGCAGAGG AGCCAGACAT GGAGGAAGAC GATGATCAGA 601 AAGCTGTGAA AGATGAACTG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 661 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 721 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCGT GGTCCATTCA 781 GGTCCCAGTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt