Construct: ORF TRCN0000491729
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002938.1_s317c1
- Derived from:
- ccsbBroadEn_10609
- DNA Barcode:
- GCCACCGCCCTTCATCTCTGACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NBPF22P (285622)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491729
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 653149 | NBPF6 | NBPF member 6 | XM_011542012.2 | 45.3% | 43.6% | (many diffs) |
| 2 | human | 653149 | NBPF6 | NBPF member 6 | XM_011542014.2 | 45.3% | 43.6% | (many diffs) |
| 3 | human | 653149 | NBPF6 | NBPF member 6 | XM_017002148.1 | 45.3% | 43.6% | (many diffs) |
| 4 | human | 653149 | NBPF6 | NBPF member 6 | NM_001143988.1 | 43.3% | 41.7% | (many diffs) |
| 5 | human | 148545 | NBPF4 | NBPF member 4 | NM_001143989.2 | 43.1% | 41.4% | (many diffs) |
| 6 | human | 653149 | NBPF6 | NBPF member 6 | XM_005271144.4 | 42.2% | 40.2% | (many diffs) |
| 7 | human | 653149 | NBPF6 | NBPF member 6 | NM_001143987.1 | 41.4% | 40% | (many diffs) |
| 8 | human | 653149 | NBPF6 | NBPF member 6 | XM_024449291.1 | 41.4% | 40% | (many diffs) |
| 9 | human | 148545 | NBPF4 | NBPF member 4 | XM_011540763.1 | 41.3% | 39.7% | (many diffs) |
| 10 | human | 285622 | NBPF22P | NBPF member 22, pseudogene | NR_003719.2 | 34.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 996
- ORF length:
- 930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg tttgggtttc ttctccccag tccctggctc tacctcttct gccacaaacg 121 tcagcatggt ggtatctgcc gaccctttgt ccagcgagag ggcagagatg aacatcctag 181 aaatcaacca ggaattgcgc tcccagctgg cagagagcaa tcagcagttc cgagacctca 241 aagagaaatt ccttatatct caagctactg cctactccct ggccaaccag ctgaagaaat 301 acaaaaagca aaatgatctt gaagaggtga aaggacaaga aacagttgct cccaggctca 361 gcaggggacc actgagagta gacaagtatg aaatccccca ggagtcactg gatggatgtt 421 gcttgactcc ttccatcctt cctgacctga ctccctccta ccacccttat tggagcactt 481 tgtactcttt tgaagacaag caagtcagct tggctcttgt agacaaaatt aaaaaggatc 541 aagaggaggt agaagaccaa agcccaccat gccccaggcT CAGCCAGGAG CGGCCAGAGG 601 TGAAGGAGCA GGAAGTCCCA GAGGACTCCG TGAATGAAGT TTACTTGACT CCCTCAGTTC 661 ACCATGATGT GTCTGACTGC CACCAGCCTT ATAGCAGCAC CTTGTCCTCA TTGGAGGATC 721 AGCTTGCCTG CTCTGCTCTG GATGTAGCCT CCCCCACCGA GGCGGCCTGT CCCCAAGGGA 781 CTTGGAGTGG AGACTTGAGC CACCACCGGT CAGAGGTTCA AATTTCACAG GCACAGCTGG 841 AACCAAGCAC CCTGGTGCCC AATTGTCTGC GACTACAGCT GGATCAAGGG TTCCACTGTG 901 GGAACGGCTT GCCCCAGCGG GGCCTTTCCT CCATGACCCG CAGCTTCTCA GCCAATGCTG 961 ATTCTGGGGA TGAAGAACCC TCCCCAGCTG GAAGATGCCC AACTTTCTTG TACAAAGTGG 1021 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1081 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1141 AGCCACCGCC CTTCATCTCT GACCCACGCG TTAAGTCgac aatcaacctc tggattacaa 1201 aatttgtgaa agatt