Construct: ORF TRCN0000491732
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009424.2_s317c1
- Derived from:
- ccsbBroadEn_15808
- DNA Barcode:
- TGCCCGGGACTGTGCATATCAGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM15B (29890)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491732
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29890 | RBM15B | RNA binding motif protein 15B | NM_013286.5 | 46.9% | 46.9% | 1_1416del |
| 2 | mouse | 109095 | Rbm15b | RNA binding motif protein 15B | NM_175402.4 | 41.4% | 44.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1320
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gggttttccc ttgggtggac cagaccgcag gctccgcgtg gattttgcca 121 aagcagagga gactcggtac ccccagcagt accagccctc gccactccct gtgcattatg 181 agctgctcac agatggatac acccggcacc gcaacctgga cgccgacctg gtgcgggaca 241 ggacgccccc acaccttctg tactcagacc gagaccggac ttttttggaa ggggactgga 301 ccagccccag taaaagctct gaccgccgaa acagccttga gggctacagt cgctcagtgc 361 gcagccggag tggtgagcgt tggggggcag atggagaccg tggtttgccc aagccctggg 421 aagagaggcg gaaacggaga agcctttcca gtgaccgtgg gaggacaacc cattcaccat 481 atgaggaacg gagtaggacc aagggcagtg ggcagcagtc agagcggggc tccgaccgca 541 cccctgagcg cagccgcaag gagaaccact ccagtgaagg gaccaaggag tccagcagca 601 actccctcag caacagcaga catggggctg aggaacgggg ccaccaccac caccaccacg 661 aggctgcaga ctcttcccac gggaagaagg caagagacag cgagcgcaat caccggacca 721 cagaggccga gcccaagcct ctggaagagc caaaacacga gaccaaaaag ctgaagaatc 781 tttcagagta cgctcagaca ctacagctgg gttggaatgg gcttctggtg ttgaaaaaca 841 gctgcttccc cacgtctatg catatcctag agggggacca gggggtgatc agcagtctcc 901 tcaaagacca cacttctggg agcaagctga cccagctgaa gatcgcccag cgccttcGAC 961 TGGACCAGCC CAAGCTTGAC GAGGTCACAC GACGCATCAA GCAGGGGAGC CCCAACGGCT 1021 ATGCGGTCCT CTTAGCCACC CAGGCAACCC CCAGTGGGCT TGGCACTGAG GGGATGCCCA 1081 CAGTAGAGCC CGGTCTGCAG AGGCGGCTTC TCAGGAACCT GGTCTCCTAC TTGAAACAGA 1141 AGCAGGCCGC AGGGGTGATC AGCTTGCCAG TGGGGGGGTC CAAGGGCAGA GACGGCACAG 1201 GCATGCTCTA CGCCTTCCCA CCCTGCGACT TTTCCCAGCA GTACCTCCAG TCAGCACTAA 1261 GGACATTGGG CAAGCTAGAA GAAGAACACA TGGTGATAGT CATCGTCAGA GACACTGCCT 1321 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGATGCCC GGGACTGTGC ATATCAGAGA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt