Construct: ORF TRCN0000491754
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003723.1_s317c1
- Derived from:
- ccsbBroadEn_09211
- DNA Barcode:
- GCGCTTTCTGTACGAGCATTCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C22orf23 (84645)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491754
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84645 | C22orf23 | chromosome 22 open reading ... | NM_032561.5 | 99.8% | 100% | 396A>G |
| 2 | human | 84645 | C22orf23 | chromosome 22 open reading ... | XM_005261781.1 | 90.9% | 87.2% | (many diffs) |
| 3 | human | 84645 | C22orf23 | chromosome 22 open reading ... | XM_005261782.3 | 90.9% | 87.2% | (many diffs) |
| 4 | human | 84645 | C22orf23 | chromosome 22 open reading ... | NM_001207062.1 | 90.1% | 89.8% | 103_104ins63;333A>G |
| 5 | human | 84645 | C22orf23 | chromosome 22 open reading ... | XM_005261784.3 | 90.1% | 89.8% | 103_104ins63;333A>G |
| 6 | human | 84645 | C22orf23 | chromosome 22 open reading ... | XM_005261783.2 | 81.8% | 77.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 717
- ORF length:
- 651
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttcacagaag cagatggagg tagtgaccaa aggaactggg ttccggcgcc 121 gccccaagac catcacttac accccgggga cctgcgagct gctcagagtg atgatgaagg 181 aatccaaact gacgaacatc cagcagcgcc acatcatgga catcatgaaa agaggagatg 241 ctttgcccct acagtgcagc ccaacatcca gccagagagt cttaccttcc aagcaaatag 301 cctcgcccat ctacctgcct cccatcctcg cagcccgtcc ccaccTCCGG CCTGCCAACA 361 TGTGTCAAGC CAATGGGGCC TACAGCCGGG AGCAGTTCAA GCCTCAAGCC ACCAGGGATT 421 TGGAGAAGGA GAAACAAAGA CTCCAAAATA TCTTTGCCAC GGGGAAGGAC ATGGAGGAAC 481 GGAAAAGAAA GGCCCCTCCT GCACGACAGA AGGCTCCAGC CCCTGAGCTA GACCGATTTG 541 AAGAGCTGGT GAAGGAAATC CAGGAGAGGA AAGAATTCCT GGCTGACATG GAGGCCCTGG 601 GACAGGGCAA ACAGTACCGA GGAATCATCC TTGCTGAAAT CTCCCAGAAA CTCCGGGAAA 661 TGGAAGACAT TGACCACAGA AGGAGTGAGG AACTTAGGAA GGGTCTTGCC ACCACTTACC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GAGCGCTTTC TGTACGAGCA TTCTACACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt