Construct: ORF TRCN0000491783
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018042.1_s317c1
- Derived from:
- ccsbBroadEn_04870
- DNA Barcode:
- CAACCGTCTACAATTCTAAAACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC58 (131076)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491783
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 131076 | CCDC58 | coiled-coil domain containi... | NM_001017928.4 | 100% | 100% | |
| 2 | human | 131076 | CCDC58 | coiled-coil domain containi... | XM_011512410.2 | 89.4% | 88.1% | (many diffs) |
| 3 | human | 131076 | CCDC58 | coiled-coil domain containi... | NM_001308326.1 | 89.3% | 88.8% | 0_1ins43;2delT;5_6delGGinsTC |
| 4 | human | 131076 | CCDC58 | coiled-coil domain containi... | XM_017005697.2 | 80.9% | 74.3% | (many diffs) |
| 5 | human | 131076 | CCDC58 | coiled-coil domain containi... | XR_924097.2 | 71.3% | (many diffs) | |
| 6 | human | 131076 | CCDC58 | coiled-coil domain containi... | XR_924096.3 | 50.5% | 1_7delGTCCAAT;332_462del;571_855del | |
| 7 | mouse | 381045 | Ccdc58 | coiled-coil domain containi... | NM_198645.2 | 94.6% | 96.5% | (many diffs) |
| 8 | mouse | 381045 | Ccdc58 | coiled-coil domain containi... | NM_001159422.1 | 86.6% | 85.4% | (many diffs) |
| 9 | mouse | 381045 | Ccdc58 | coiled-coil domain containi... | NM_001159421.1 | 66.2% | 67.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 501
- ORF length:
- 432
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcgccc agtggcggtg tgaactgtga ggagttcgcc gagttccagg 121 aattactcaa ggtgatgagg acaattgatg acagaatagt acatgaatta aacactacgg 181 ttccaacagc ttcctttgca gggaaaattg atgccagcca aacctgtaaa caactttatg 241 agtctttGAT GGCAGCTCAT GCCAGTAGAG ACAGAGTCAT AAAAAACTGT ATAGCCCAGA 301 CTTCAGCAGT AGTAAAAAAC CTCCGAGAAG AGAGAGAAAA GAATTTGGAC GATTTAACGT 361 TATTAAAACA ACTTAGAAAA GAGCAGACAA AGTTGAAATG GATGCAGTCA GAACTGAATG 421 TTGAAGAAGT GGTAAATGAC AGGAGCTGGA AGGTGTTTAA TGAACGCTGC CGAATTCACT 481 TCAAGCCTCC AAAGAATGAA TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACAAC CGTCTACAAT 661 TCTAAAACAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt