Construct: ORF TRCN0000491785
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007790.1_s317c1
- Derived from:
- ccsbBroadEn_03858
- DNA Barcode:
- AGACTTCGCCCGACGGAGCAGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRPRB (58477)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491785
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58477 | SRPRB | SRP receptor subunit beta | NM_021203.4 | 100% | 100% | |
| 2 | human | 58477 | SRPRB | SRP receptor subunit beta | NR_163491.1 | 19.8% | 1_34del;848_4087del | |
| 3 | mouse | 20818 | Srprb | signal recognition particle... | NM_009275.4 | 85.1% | 90.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 879
- ORF length:
- 813
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttccgcggac tcgcgccggg tggcagatgg cggcggtgcc gggggcacct 121 tccagcccta cctagacacc ttgcggcagg agctgcagca gacggaccca acgctgttgt 181 cagtagtggt ggcggttctt gcggtgctgc tgacgctagt cttctggaag ttaatccgga 241 gcagaaggag cagtcagaga gctgttcttc ttgttggcct ttgtgattcc gggaaaacgt 301 tgctctttgt caggttgtta acaggccttt atagagacac tcagacgtcc attactgaca 361 gctgtgctgt atacagagtc aacaataaca ggggcaatag tctgaccttg attgaccttc 421 ccggccatga gagtttgagg cttcagttct tagagcggtt taagtcttca gccagggcta 481 ttgtgtttgt tgtggatagt gcagcattcc agcgagaggt gaaagatgtg gctgagtttc 541 tgtatcaagt cctcattgac agtatgggtc tgaagaatac accatcattc ttaatagccT 601 GCAATAAGCA AGATATTGCA ATGGCAAAAT CAGCAAAGTT AATTCAACAG CAGCTGGAGA 661 AAGAACTCAA CACCTTACGA GTTACCCGTT CTGCTGCCCC CAGCACACTG GACAGTTCCA 721 GCACTGCCCC TGCTCAGCTG GGGAAGAAAG GCAAAGAGTT TGAATTCTCA CAGTTGCCCC 781 TCAAAGTGGA GTTCCTGGAG TGCAGTGCCA AGGGTGGAAG AGGGGACGTG GGCTCTGCTG 841 ACATCCAGGA CTTGGAGAAA TGGCTGGCTA AAATTGCCTG CCCAACTTTC TTGTACAAAG 901 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 961 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1021 ACGAAGACTT CGCCCGACGG AGCAGGACAC GCGTTAAGTC gacaatcaac ctctggatta 1081 caaaatttgt gaaagatt