Construct: ORF TRCN0000491786
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012256.1_s317c1
- Derived from:
- ccsbBroadEn_12361
- DNA Barcode:
- TGGTACGGCATCTGGCAAGCATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIAA1324 (57535)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491786
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57535 | KIAA1324 | KIAA1324 | NM_001267048.2 | 99.6% | 99.5% | (many diffs) |
| 2 | human | 57535 | KIAA1324 | KIAA1324 | XM_011541827.2 | 95.7% | 95.7% | (many diffs) |
| 3 | human | 57535 | KIAA1324 | KIAA1324 | NM_020775.5 | 91% | 91% | (many diffs) |
| 4 | human | 57535 | KIAA1324 | KIAA1324 | XM_011541825.2 | 89.3% | 89.1% | (many diffs) |
| 5 | human | 57535 | KIAA1324 | KIAA1324 | XM_011541826.3 | 89.1% | 88.9% | (many diffs) |
| 6 | human | 57535 | KIAA1324 | KIAA1324 | NM_001284352.2 | 79.3% | 79.1% | (many diffs) |
| 7 | human | 57535 | KIAA1324 | KIAA1324 | XM_017001883.2 | 73.3% | 70.6% | (many diffs) |
| 8 | human | 57535 | KIAA1324 | KIAA1324 | NM_001284353.1 | 72.1% | 72% | (many diffs) |
| 9 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | NM_001346518.1 | 80.1% | 82.6% | (many diffs) |
| 10 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | XM_006501412.2 | 78.6% | 80.7% | (many diffs) |
| 11 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | XM_006501413.2 | 78% | 80.5% | (many diffs) |
| 12 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | NM_001033304.1 | 71.3% | 73.8% | (many diffs) |
| 13 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | XM_006501414.3 | 71.3% | 73.8% | (many diffs) |
| 14 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | XM_006501415.3 | 71.3% | 73.8% | (many diffs) |
| 15 | mouse | 229722 | 5330417C22Rik | RIKEN cDNA 5330417C22 gene | XM_017319560.1 | 69.8% | 71.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2847
- ORF length:
- 2778
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgagcct gggcacagcc accatctctc cgccagagtc aggggaagaa 121 ctgagaggcg cataccccgg ctgtggcggc tgctgctctg ggctgggacc gccttccagg 181 tgacccaggg aacgggaccg gagcttcacg cctgcaaaga gtctgagtac cactatgagt 241 acacggcgtg tgacagcacg ggttccaggt ggagggtcgc cgtgccgcat accccgggcc 301 tgtgcaccag cctgcctgac cccgtcaagg gcaccgagtg ctccttctcc tgcaacgccg 361 gggagtttct ggatatgaag gaccagtcat gtaagccatg cgctgagggc cgctactccc 421 tcggcacagg cattcggttt gatgagtggg atgagctgcc ccatggcttt gccagcctct 481 cagccaacat ggagctggat gacagtgctg ctgagtccac cgggaactgt acttcgtcca 541 agtgggttcc ccggggcgac tacatcgcct ccaacacgga cgaatgcaca gccacactga 601 tgtacgccgt caacctgaag caatctggca ccgttaactt cgaatactac tatccagact 661 ccagcatcat ctttgagttt ttcgttcaga atgaccagtg ccagcccaat gcagatgact 721 ccaggtggat gaagaccaca gagaaaggat gggaattcca cagtgtggag ctaaatcgag 781 gcaaaaatgt cctctattgg agaaccacag ccttctcagt atggaccaaa gtacccaagc 841 ctgtgctgac acaactcatg tacaaatggg ccaagccgaa aatctgtagc gaggaccttg 901 agggggcagt gaagctgcct gcctctggtg tgaagaccca ctgcccaccc tgcaacccag 961 gcttcttcaa aaccaacaac agcacctgcc agccctgccc atatggttcc tactccaatg 1021 gctcagactg tacccgctgc cctgcaggga ctgaacctgc tgtgggattt gaatacaaat 1081 ggtggaacac gctgcccaca aacatggaaa cgaccgttct cagtgggatc aacttcgagt 1141 acaagggcat gacaggctgg gaggtggctg gtgatcacat ttacacagct gctggagcct 1201 cagacaatga cttcatgatt ctcactctgg ttgtgccagg atttagacct ccgcagtcgg 1261 tgatggcaga cacagagaat aaagaggtgg ccagaatcac atttgtcttt gagaccctct 1321 gttctgtgaa ctgtgagctc tacttcatgg tgggtgtgaa ttctaggacc aacactcctg 1381 tggagacgtg gaaaggttcc aaaggcaaac agtcctatac ctacatcatt gaggagaaca 1441 ctaccacgag cttcacctgg gccttccaga ggaccacttt tcatgaggca agcaggaagt 1501 acaccaatga cgttgccaag atctactcca tcaatgtcac caatgttatg aatggcgtgg 1561 cctcctactg ccgtccctgt gccctagaag cctctgatgt gggctcctcc tgcacctctt 1621 gtcctgctgg ttactatatt gaccgagatt caggaacctg ccactcctgc ccccctaaca 1681 caattctgaa agcccaccag ccttatggtg tccaggcctg tgtgccctgt ggtccaggga 1741 ccaagaacaa caagatccac tctctgtgct acaatgattg caccttctca cgcaacactc 1801 caaccaggac tttcaactac aacttctccg ctttggcaaa caccgtcact cttgctggag 1861 ggccaagctt cacttccaaa gggttgaaat acttccatca ctttaccctc agtctctgtg 1921 gaaaccaggg taggaaaatg tctgtgtgca ccgacaatgt cactgacctc cggattcctg 1981 agggtgagtc agggttctcc aaatctatca cagcctacgt ctgccaggca gtcatcatcc 2041 ccccagaggt gacaggctac aaggccgggg tttcctcaca gcctgtcagc cttgctgatc 2101 gacttattgg ggtgacaaca gatatgactc tggatggaat cacctcccca gctgaacttt 2161 tccacctgga gtccttggga ataccggacg tgatcttctt ttataggtcc aatgatgtga 2221 cccagtcctg cagttctggg agatcaacca ccatccgcgt caggtgcagt ccacagaaaa 2281 ctgtccctgg aagtttgctg ctgccaggaa cgtgctcaga tgggacctgt gatggctgca 2341 acttccactt cctgtgggag agcgcggctg cttgcccgct ctgctcagtg gctgactacc 2401 atgctatcgt cagcagctgt gtggctggga tccagaagac tacttacgtg tggcgagaac 2461 ccaagctatg ctctggtggc atttctctgc cTGAGCAGAG AGTCACCATC TGCAAAACCA 2521 TAGATTTCTG GCTGAAAGTG GGCATCTCTG CAGGCACCTG TACTGCCATC CTGCTCACCG 2581 TCTTGACCTG CTACTTTTGG AAAAAGAATC AAAAACTAGA GTACAAGTAC TCCAAGCTGG 2641 TGATGAATGC TACTCTCAAG GACTGTGACC TGCCAGCAGC TGACAGCTGC GCCATCATGG 2701 AAGGCGAGGA TGTAGAGGAC GACCTCATCT TTACCAGCAA GAAGTCACTC TTTGGGAAGA 2761 TCAAATCATT TACCTCCAAG AGGACTCCTG ATGGATTTGA CTCAGTGCCG CTGAAGACAT 2821 CCTCAGGAGG CCCAGACATG GACCTGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2881 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2941 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGGTACGG 3001 CATCTGGCAA GCATCCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3061 aagatt