Construct: ORF TRCN0000491790
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008925.1_s317c1
- Derived from:
- ccsbBroadEn_13396
- DNA Barcode:
- TCGAAGAATGATTGTGTCCGACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADCK5 (203054)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491790
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 203054 | ADCK5 | aarF domain containing kina... | XM_006716527.2 | 35.7% | 35.7% | 1_912del |
| 2 | human | 203054 | ADCK5 | aarF domain containing kina... | XM_011516910.1 | 35.7% | 35.7% | 1_912del |
| 3 | human | 203054 | ADCK5 | aarF domain containing kina... | XM_011516909.1 | 32.9% | 32.9% | 1_1032del |
| 4 | human | 203054 | ADCK5 | aarF domain containing kina... | XM_017013174.1 | 32.9% | 32.9% | 1_1032del |
| 5 | human | 203054 | ADCK5 | aarF domain containing kina... | NM_174922.5 | 29.1% | 29.1% | 1_1233del |
| 6 | human | 203054 | ADCK5 | aarF domain containing kina... | XM_011516907.3 | 25.8% | 25.8% | 1_1455del |
| 7 | human | 203054 | ADCK5 | aarF domain containing kina... | XR_002956603.1 | 24.2% | 1_1413del;1921_2091del | |
| 8 | human | 203054 | ADCK5 | aarF domain containing kina... | XR_928305.3 | 18.7% | 1_2021del;2529_2699del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ggcgcacgca gccgcactgg gggtgcaaga ctacctcctg ttcgccgaga 121 tgctcatgca gcgccccgtg cgcctggggc agctgtgggg ctcgcaccta ctgagccgcg 181 aagaggcggc ctacatggtg gacatggccc gcgagcgctt cgaggccgtc atggcggtgc 241 tcagggagct gccgcggccc atgctgctgg tgctgcgcaa catcaacacc gtgcgcgcta 301 tcaacgtggc cctcggcgcc cccgtggacc gctacttcct tatggctaaa agggctgtcc 361 ggggctggag ccgcctggcg ggcgccacgt atcggggtgt cTACGGCACC AGCCTCCTGC 421 GCCACGCCAA GGTCGTCTGG GAGATGCTCA AGTTTGAAGT GGCGCTCAGG CTGGAGACCT 481 TGGCCATGCG GCTGACCGCC CTCCTGGCTC GTGCTCTGGT CCACCTGAGC CTCGTGCCCC 541 CAGCGGAGGA GCTCTACCAG TACCTGGAGA CCTACCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 721 GAAGAATGAT TGTGTCCGAC AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt