Construct: ORF TRCN0000491799
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021582.2_s317c1
- DNA Barcode:
- CTGCAATTATTATATTTTCTCCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K3 (5606)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491799
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5606 | MAP2K3 | mitogen-activated protein k... | NM_145109.3 | 99.9% | 100% | 360T>C |
| 2 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_017024857.2 | 97.5% | 95.9% | (many diffs) |
| 3 | human | 5606 | MAP2K3 | mitogen-activated protein k... | NM_001316332.2 | 91.5% | 91.6% | 0_1ins87;273T>C |
| 4 | human | 5606 | MAP2K3 | mitogen-activated protein k... | NM_002756.4 | 91.5% | 91.6% | 0_1ins87;273T>C |
| 5 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_005256723.2 | 91.5% | 91.6% | 0_1ins87;273T>C |
| 6 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_011523959.2 | 91.5% | 91.6% | 0_1ins87;273T>C |
| 7 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_017024858.1 | 91.5% | 91.6% | 0_1ins87;273T>C |
| 8 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_017024859.1 | 91.5% | 91.6% | 0_1ins87;273T>C |
| 9 | human | 5606 | MAP2K3 | mitogen-activated protein k... | XM_011523958.2 | 72.5% | 66.1% | (many diffs) |
| 10 | mouse | 26397 | Map2k3 | mitogen-activated protein k... | NM_008928.4 | 90.7% | 96.5% | (many diffs) |
| 11 | mouse | 26397 | Map2k3 | mitogen-activated protein k... | XM_006533368.3 | 67.6% | 72.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1110
- ORF length:
- 1041
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagtcgccc gcctcgagcc agcccgccag catgccccag tccaaaggaa 121 aatccaagag gaagaaggat ctacggatat cctgcatgtc caagccaccc gcacccaacc 181 ccacaccccc ccggaacctg gactcccgga ccttcatcac cattggagac agaaactttg 241 aggtggaggc tgatgacttg gtgaccatct cagaactggg ccgtggagcc tatggggtgg 301 tagagaaggt gcggcacgcc cagagcggca ccatcatggc cgtgaagcgg atccgggcca 361 ccgtgaactc acaggagcag aagcggctgc tcatggacct ggacatcaac atgcgcacgg 421 tcgactgctt ctacactgtc accttctacg gggcactatt cagagaggga gacgtgtgga 481 tctgcatgga gctcatggac acatccttgg acaagttcta ccggaaggtg ctggataaaa 541 acatgacaat tccagaggac atccttgggg agattgctgt gtctatcgtg cgggccctgg 601 agcatctgca cagcaagctg tcggtgatcc acagagatgt gaagccctcc aatgtcctta 661 tcaacaagga gggccatgtg aagatgtgtg actttggcat cagtggctac ttggtggact 721 ctgtggccaa gacgatggat gccggctgca agccctacat ggCCCCTGAG AGGATCAACC 781 CAGAGCTGAA CCAGAAGGGC TACAATGTCA AGTCCGACGT CTGGAGCCTG GGCATCACCA 841 TGATTGAGAT GGCCATCCTG CGGTTCCCTT ACGAGTCCTG GGGGACCCCG TTCCAGCAGC 901 TGAAGCAGGT GGTGGAGGAG CCGTCCCCCC AGCTCCCAGC CGACCGTTTC TCCCCCGAGT 961 TTGTGGACTT CACTGCTCAG TGCCTGAGGA AGAACCCCGC AGAGCGTATG AGCTACCTGG 1021 AGCTGATGGA GCACCCCTTC TTCACCTTGC ACAAAACCAA GAAGACGGAC ATTGCTGCCT 1081 TCGTGAAGGA GATCCTGGGA GAAGACTCAT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 1141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 1261 GCAATTATTA TATTTTCTCC ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 1321 ttgtgaaaga tt