Construct: ORF TRCN0000491802
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021259.2_s317c1
- DNA Barcode:
- GTTCAGTCTTTCAGAGAATAGACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PTPN22 (26191)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491802
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26191 | PTPN22 | protein tyrosine phosphatas... | XM_017001006.1 | 54.8% | 50.9% | (many diffs) |
| 2 | human | 26191 | PTPN22 | protein tyrosine phosphatas... | XM_011541223.2 | 23.9% | 22.2% | (many diffs) |
| 3 | human | 26191 | PTPN22 | protein tyrosine phosphatas... | XM_011541225.2 | 22.8% | 21.2% | (many diffs) |
| 4 | human | 26191 | PTPN22 | protein tyrosine phosphatas... | NM_012411.5 | 22.7% | 21.1% | (many diffs) |
| 5 | human | 26191 | PTPN22 | protein tyrosine phosphatas... | XM_017001004.1 | 22.1% | 20.5% | (many diffs) |
| 6 | human | 26191 | PTPN22 | protein tyrosine phosphatas... | XM_011541222.1 | 22.1% | 20.5% | (many diffs) |
| 7 | human | 26191 | PTPN22 | protein tyrosine phosphatas... | NM_001193431.2 | 21.9% | 20.3% | (many diffs) |
| 8 | human | 26191 | PTPN22 | protein tyrosine phosphatas... | XM_011541221.1 | 21.9% | 20.3% | (many diffs) |
| 9 | human | 26191 | PTPN22 | protein tyrosine phosphatas... | NM_001308297.1 | 21.8% | 20.2% | (many diffs) |
| 10 | human | 26191 | PTPN22 | protein tyrosine phosphatas... | NM_015967.7 | 21.1% | 19.6% | (many diffs) |
| 11 | mouse | 19260 | Ptpn22 | protein tyrosine phosphatas... | NM_008979.2 | 18.6% | 17% | (many diffs) |
| 12 | mouse | 19260 | Ptpn22 | protein tyrosine phosphatas... | XM_006501148.3 | 18.6% | 17% | (many diffs) |
| 13 | mouse | 19260 | Ptpn22 | protein tyrosine phosphatas... | XM_006501149.3 | 18.6% | 17% | (many diffs) |
| 14 | mouse | 19260 | Ptpn22 | protein tyrosine phosphatas... | XM_006501150.2 | 18.6% | 17% | (many diffs) |
| 15 | mouse | 19260 | Ptpn22 | protein tyrosine phosphatas... | XM_011240041.2 | 18.6% | 17% | (many diffs) |
| 16 | mouse | 19260 | Ptpn22 | protein tyrosine phosphatas... | XM_011240042.2 | 18.6% | 17% | (many diffs) |
| 17 | mouse | 19260 | Ptpn22 | protein tyrosine phosphatas... | NR_104070.1 | 16.2% | (many diffs) | |
| 18 | mouse | 19260 | Ptpn22 | protein tyrosine phosphatas... | XR_375500.3 | 7.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 609
- ORF length:
- 537
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaccaa agagaaattc tgcagaagtt cctggatgag gcccaaagca 121 agaaaattac taaagaggag tttgccaatg aatttctgaa gctgaaaagg caatctacca 181 agtacaaggc agacaaaacc tatcctacaa ctgtggctga gaagcccaag aatatcaaga 241 aaaacagata taaggatatt ttgccctatg attatagccg gGTAGAACTA TCCCTGATAA 301 CCTCTGATGA GGATTCCAGC TACATCAATG CCAACTTCAT TAAGGGAGTT TATGGACCCA 361 AGGCTTATAT TGCCACCCAG GGTCCTTTAT CTACAACCCT CCTGGACTTC TGGAGGATGA 421 TTTGGGAATA TAGTGTCCTT ATCATTGTTA TGGCATGCAT GGAGTATGAA ATGGGAAAGG 481 AAGCTGAAAA AAGGAAATCT GATTATATAA TCAGGACTCT AAAAGTTAAG TTCAATAGTG 541 TATCTGTAAT TCTTGCCCAC CAAACAAGCC TGCAGAATCT GTTCAGTCAA ATAACTCCAG 601 CTCATTTCTA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 661 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 721 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTT CAGTCTTTCA GAGAATAGAC 781 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t