Construct: ORF TRCN0000491803
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017339.1_s317c1
- Derived from:
- ccsbBroadEn_07442
- DNA Barcode:
- TTATCCCGTGAAAGACGACAATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB3D (9545)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491803
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9545 | RAB3D | RAB3D, member RAS oncogene ... | NM_004283.4 | 99.8% | 99.5% | 7T>G |
| 2 | mouse | 19340 | Rab3d | RAB3D, member RAS oncogene ... | NM_001324531.1 | 87.2% | 94% | (many diffs) |
| 3 | mouse | 19340 | Rab3d | RAB3D, member RAS oncogene ... | NM_031874.5 | 87.2% | 94% | (many diffs) |
| 4 | mouse | 19340 | Rab3d | RAB3D, member RAS oncogene ... | XM_011242422.2 | 87.2% | 94% | (many diffs) |
| 5 | mouse | 19340 | Rab3d | RAB3D, member RAS oncogene ... | XM_011242423.2 | 87.2% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 723
- ORF length:
- 657
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcagctgga gacacccagg caggcccacg ggatgcagca gatcagaact 121 tcgactatat gttcaaactg ctactgatag gcaacagcag tgtgggcaag acttccttcc 181 tgttccgata cgcggacgac tccttcactc ccgccttcgt cagtactgtg ggcatcgatt 241 tcaaggtcaa gaccgtctac cgccatgaca agaggatcaa gctgcagatc tgggacacag 301 cgggccagga gcgctaccgc accatcacca cggcctacta ccggggagcc atgggcttcc 361 tgctcatgta tgacatcgcc aatcaggaat cctttgccgc tgtgcaggac tGGGCCACGC 421 AAATCAAGAC CTACTCCTGG GACAACGCCC AGGTCATCCT GGTGGGGAAC AAGTGTGACC 481 TGGAGGACGA ACGTGTTGTG CCTGCTGAGG ATGGCCGGAG GCTCGCCGAC GACCTTGGTT 541 TCGAGTTCTT TGAAGCCAGT GCCAAGGAGA ACATCAATGT GAAGCAGGTC TTCGAGCGCC 601 TGGTGGATGT CATCTGCGAG AAGATGAACG AGTCCCTGGA ACCCAGCTCC AGCTCAGGCA 661 GCAACGGGAA AGGCCCGGCC GTGGGGGATG CTCCAGCCCC CCAGCCCAGC AGCTGCAGCT 721 GCTCCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 781 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 841 GGCTTTATAT ATCTTGTGGA AAGGACGATT ATCCCGTGAA AGACGACAAT TCACGCGTTA 901 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt