Construct: ORF TRCN0000491806
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006938.2_s317c1
- Derived from:
- ccsbBroadEn_09609
- DNA Barcode:
- TCGGTACGTCCCATTGGTAGTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AMDHD1 (144193)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491806
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144193 | AMDHD1 | amidohydrolase domain conta... | NM_152435.3 | 99.6% | 99.7% | (many diffs) |
| 2 | mouse | 71761 | Amdhd1 | amidohydrolase domain conta... | NM_027908.1 | 87.5% | 88.9% | (many diffs) |
| 3 | mouse | 71761 | Amdhd1 | amidohydrolase domain conta... | XM_006514167.3 | 72.3% | 71.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1344
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aggcggccac agcctcctgc tggagaacgc gcagcaagtg gtgttggtgt 121 gcgcccgcgg cgagcgcttc ctggcgcggg atgcgctgcg cagcctggcg gtgctggaag 181 gcgccagtct ggtggtgggc aaagatggat ttataaaagc tattggtcct gctgatgtta 241 ttcaaagaca gttttctgga gaaacttttg aagaaataat tgactgctct gggaaatgta 301 tcctaccagg tttggtggat gcacacacac atccagtatg ggctggtgaa agagttcacg 361 aatttgcaat gaagttggca ggagccacct acatggaaat tcaccaggcc ggaggaggga 421 tccactttac cgtggagcgc acgcgccaag ccacagagga ggagctgttc cgctccttgc 481 agcaacggct ccagtgcatg atgagggctg gcaccacgct ggtggagtgc aagagtggat 541 atggcctcga cctggagacc gagctcaaga tgctgcgcgt gattgagcgc gcccggcggg 601 agctggacat cggcatctcg gctacctact gcggggctca ttcagtgcct aaaggaaaaa 661 ctgctactga agctgctgat gacatcatca ataaccacct cccaaagctg aaggaacttg 721 gcagaaatgg ggaaatacac gtggacaata tagacgtatt ttgtgagaaa ggtgtctttg 781 atctcgattc caccagaagg attcttcaac gtggaaaaga tatagggtta cagattaact 841 tccatgggga tgaactccac ccgatgaagg ctgctgagct tggggctgaa ctgggagcgc 901 aggcaatcag ccacctggaa gaagtgagtg atgaaggcat cgttgccatg gcaacggcca 961 ggtgctctgc catccttctg cccaccacag ccTACATGCT GAGACTGAAA CAACCTCGAG 1021 CCAGGAAGAT GTTAGATGAA GGAGTAATAG TTGCTCTGGG AAGTGATTTC AACCCCAATG 1081 CATATTGCTT TTCAATGCCA ATGGTCATGC ATCTGGCCTG TGTAAACATG AGAATGTCCA 1141 TGCCTGAGGC CTTGGCCGCT GCCACCATCA ATGCAGCTTA TGCACTGGGA AAGTCTCACA 1201 CACACGGATC GTTGGAAGTT GGCAAACAGG GAGATCTCAT TATCATCAAT TCATCCCGAT 1261 GGGAGCATTT GATTTACCAG TTCGGAGGCC ATCATGAATT AATTGAATAT GTTATAGCTA 1321 AAGGAAAACT CATCTATAAA ACATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1381 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1441 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CGGTACGTCC 1501 CATTGGTAGT ATCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1561 att