Construct: ORF TRCN0000491814
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020143.2_s317c1
- DNA Barcode:
- GCGATCTATGAGTGTCTGACCAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- AKT1 (207)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491814
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 207 | AKT1 | AKT serine/threonine kinase 1 | NM_001014431.2 | 99.9% | 100% | 84C>A |
| 2 | human | 207 | AKT1 | AKT serine/threonine kinase 1 | NM_001014432.1 | 99.9% | 100% | 84C>A |
| 3 | human | 207 | AKT1 | AKT serine/threonine kinase 1 | NM_005163.2 | 99.9% | 100% | 84C>A |
| 4 | human | 207 | AKT1 | AKT serine/threonine kinase 1 | XR_002957536.1 | 32.9% | (many diffs) | |
| 5 | mouse | 11651 | Akt1 | thymoma viral proto-oncogene 1 | NM_001331107.1 | 90.8% | 98.3% | (many diffs) |
| 6 | mouse | 11651 | Akt1 | thymoma viral proto-oncogene 1 | NM_009652.3 | 90.8% | 98.3% | (many diffs) |
| 7 | mouse | 11651 | Akt1 | thymoma viral proto-oncogene 1 | XM_006515415.1 | 90.8% | 98.3% | (many diffs) |
| 8 | mouse | 11651 | Akt1 | thymoma viral proto-oncogene 1 | NM_001165894.1 | 83.2% | 90.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1512
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagcgac gtggctattg tgaaggaggg ttggctgcac aaacgagggg 121 agtacatcaa gacctggcgg ccacgctact tcctactcaa gaatgatggc accttcattg 181 gctacaagga gcggccgcag gatgtggacc aacgtgaggc tcccctcaac aacttctctg 241 tggcgcagtg ccagctgatg aagacggagc ggccccggcc caacaccttc atcatccgct 301 gcctgcagtg gaccactgtc atcgaacgca ccttccatgt ggagactcct gaggagcggg 361 aggagtggac aaccgccatc cagactgtgg ctgacggcct caagaagcag gaggaggagg 421 agatggactt ccggtcgggc tcacccagtg acaactcagg ggctgaagag atggaggtgt 481 ccctggccaa gcccaagcac cgcgtgacca tgaacgagtt tgagtacctg aagctgctgg 541 gcaagggcac tttcggcaag gtgatcctgg tgaaggagaa ggccacaggc cgctactacg 601 ccatgaagat cctcaagaag gaagtcatcg tggccaagga cgaggtggcc cacacactca 661 ccgagaaccg cgtcctgcag aactccaggc accccttcct cacagccctg aagtactctt 721 tccagaccca cgaccgcctc tgctttgtca tggagtacgc caacgggggc gagctgttct 781 tccacctgtc ccgggagcgt gtgttctccg aggaccgggc ccgcttctat ggcgctgaga 841 ttgtgtcagc cctggactac ctgcactcgg agaagaacgt ggtgtaccgg gacctcaagc 901 tggagaacct catgctggac aaggacgggc acattaagat cacagacttc gggctgtgca 961 aggaggggat caaggacggt gccaccatga agaccttttg cggcacacct gagtacctgg 1021 cccccgaggt gctggaggac aatgactacg gccgtgcagt ggactggtgg gggctgggcg 1081 tggtcatgta cgagatgatg tgcggtcgcc tgcccttcta caaccaggac catgagaagc 1141 tttttgagct catccTCATG GAGGAGATCC GCTTCCCGCG CACGCTTGGT CCCGAGGCCA 1201 AGTCCTTGCT TTCAGGGCTG CTCAAGAAGG ACCCCAAGCA GAGGCTTGGC GGGGGCTCCG 1261 AGGACGCCAA GGAGATCATG CAGCATCGCT TCTTTGCCGG TATCGTGTGG CAGCACGTGT 1321 ACGAGAAGAA GCTCAGCCCA CCCTTCAAGC CCCAGGTCAC GTCGGAGACT GACACCAGGT 1381 ATTTTGATGA GGAGTTCACG GCCCAGATGA TCACCATCAC ACCACCTGAC CAAGATGACA 1441 GCATGGAGTG TGTGGACAGC GAGCGCAGGC CCCACTTCCC CCAGTTCTCC TACTCGGCCA 1501 GCGGCACGGC CTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1561 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1621 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCGATCTATG AGTGTCTGAC 1681 CAGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt