Construct: ORF TRCN0000491816
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019148.2_s317c1
- DNA Barcode:
- GCTACCTCGTGCGTTACTCATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FSHR (2492)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491816
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2492 | FSHR | follicle stimulating hormon... | NM_000145.4 | 100% | 100% | |
| 2 | human | 2492 | FSHR | follicle stimulating hormon... | NM_181446.3 | 96.2% | 96.2% | 444_445ins78 |
| 3 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532733.2 | 95.3% | 95.3% | 668_769del |
| 4 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532734.2 | 78.8% | 77.7% | (many diffs) |
| 5 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532735.2 | 62% | 62% | 0_1ins792 |
| 6 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532736.2 | 62% | 62% | 0_1ins792 |
| 7 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532740.1 | 39% | 39% | 668_769del;957_957delAins1231 |
| 8 | mouse | 14309 | Fshr | follicle stimulating hormon... | NM_013523.3 | 86.2% | 87.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2160
- ORF length:
- 2085
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcc ctgctcctgg tctctttgct ggcattcctg agcttgggct 121 caggatgtca tcatcggatc tgtcactgct ctaacagggt ttttctctgc caagagagca 181 aggtgacaga gattccttct gacctcccga ggaatgccat tgaactgagg tttgtcctca 241 ccaagcttcg agtcatccaa aaaggtgcat tttcaggatt tggggacctg gagaaaatag 301 agatctctca gaatgatgtc ttggaggtga tagaggcaga tgtgttctcc aaccttccca 361 aattacatga aattagaatt gaaaaggcca acaacctgct ctacatcaac cctgaggcct 421 tccagaacct tcccaacctt caatatctgt taatatccaa cacaggtatt aagcaccttc 481 cagatgttca caagattcat tctctccaaa aagttttact tgacattcaa gataacataa 541 acatccacac aattgaaaga aattctttcg tggggctgag ctttgaaagt gtgattctat 601 ggctgaataa gaatgggatt caagaaatac acaactgtgc attcaatgga acccaactag 661 atgagctgaa tctaagcgat aataataatt tagaagaatt gcctaatgat gttttccacg 721 gagcctctgg accagtcatt ctagatattt caagaacaag gatccattcc ctgcctagct 781 atggcttaga aaatcttaag aagctgaggg ccaggtcgac ttacaactta aaaaagctgc 841 ctactctgga aaagcttgtc gccctcatgg aagccagcct cacctatccc agccattgct 901 gtgcctttgc aaactggaga cggcaaatct ctgagcttca tccaatttgc aacaaatcta 961 ttttaaggca agaagttgat tatatgactc aggctagggg tcagagatcc tctctggcag 1021 aagacaatga gtccagctac agcagaggat ttgacatgac gtacactgag tttgactatg 1081 acttatgcaa tgaagtggtt gacgtgacct gctcccctaa gccagatgca ttcaacccat 1141 gtgaagatat catggggtac aacatcctca gagtcctgat atggtttatc agcatcctgg 1201 ccatcactgg gaacatcata gtgctagtga tcctaactac cagccaatat aaactcacag 1261 tccccaggtt ccttatgtgc aacctggcct ttgctgatct ctgcattgga atctacctgc 1321 tgctcattgc atcagttgat atccatacca agagccaata tcacaactat gccattgact 1381 ggcaaactgg ggcaggctgt gatgctgctg gctttttcac tgtctttgcc agtgagctgt 1441 cagtctacac tctgacagct atcaccttgg aaagatggca taccatcacg catgccatgc 1501 agctggactg caaggtgcag ctccgccatg ctgccagtgt catggtgatg ggctggattt 1561 ttgcttttgc agctgccctc tttcccatct ttggcatcag cagctacatg aaggtgagca 1621 tctgcctgcc catggatatt gacagccctt tgtcacagct gtatgtcatg tccctccttg 1681 tgctcaatgt cctggccttt gtggtcatct gtggctgcta tatccacatc tacctcacag 1741 tgcggaaccc caacatcgtg tccTCCTCTA GTGACACCAG GATCGCCAAG CGCATGGCCA 1801 TGCTCATCTT CACTGACTTC CTCTGCATGG CACCCATTTC TTTCTTTGCC ATTTCTGCCT 1861 CCCTCAAGGT GCCCCTCATC ACTGTGTCCA AAGCAAAGAT TCTGCTGGTT CTGTTTCACC 1921 CCATCAACTC CTGTGCCAAC CCCTTCCTCT ATGCCATCTT TACCAAAAAC TTTCGCAGAG 1981 ATTTCTTCAT TCTGCTGAGC AAGTGTGGCT GCTATGAAAT GCAAGCCCAA ATTTATAGGA 2041 CAGAAACTTC ATCCACTGTC CACAACACCC ATCCAAGGAA TGGCCACTGC TCTTCAGCTC 2101 CCAGAGTCAC CAGTGGTTCC ACTTACATAC TTGTCCCTCT AAGTCATTTA GCCCAAAACG 2161 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2221 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2281 TTTATATATC TTGTGGAAAG GACGAGCTAC CTCGTGCGTT ACTCATTTAA CGCGTTAAGT 2341 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt