Construct: ORF TRCN0000491819
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019843.2_s317c1
- DNA Barcode:
- GTTAAGTTCGACAGAAATATTGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FADS2 (9415)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491819
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9415 | FADS2 | fatty acid desaturase 2 | NM_004265.4 | 99.9% | 99.7% | 1332_1333insG |
| 2 | human | 9415 | FADS2 | fatty acid desaturase 2 | NM_001281501.1 | 90.3% | 83.8% | (many diffs) |
| 3 | human | 9415 | FADS2 | fatty acid desaturase 2 | NM_001281502.1 | 88.5% | 82.2% | (many diffs) |
| 4 | mouse | 56473 | Fads2 | fatty acid desaturase 2 | NM_019699.1 | 87.1% | 87.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1404
- ORF length:
- 1335
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggggaaggga gggaaccagg gcgagggggc cgccgagcgc gaggtgtcgg 121 tgcccacctt cagctgggag gagattcaga agcataacct gcgcaccgac aggtggctgg 181 tcattgaccg caaggtttac aacatcacca aatggtccat ccagcacccg gggggccagc 241 gggtcatcgg gcactacgct ggagaagatg caacggatgc cttccgcgcc ttccaccctg 301 acctggaatt cgtgggcaag ttcttgaaac ccctgctgat tggtgaactg gccccggagg 361 agcccagcca ggaccacggc aagaactcaa agatcactga ggacttccgg gccctgagga 421 agacggctga ggacatgaac ctgttcaaga ccaaccacgt gttcttcctc ctcctcctgg 481 cccacatcat cgccctggag agcattgcat ggttcactgt cttttacttt ggcaatggct 541 ggattcctac cctcatcacg gcctttgtcc ttgctacctc tcaggcccaa gctggatggc 601 tgcaacatga ttatggccac ctgtctgtct acagaaaacc caagtggaac caccttgtcc 661 acaaattcgt cattggccac ttaaagggtg cctctgccaa ctggtggaat catcgccact 721 tccagcacca cgccaagcct aacatcttcc acaaggatcc cgatgtgaac atgctgcacg 781 tgtttgttct gggcgaatgg cagcccatcg agtacggcaa gaagaagctg aaatacctgc 841 cctacaatca ccagcacgaa tacttcttcc tgattgggcc gccgctgctc atccccatgt 901 atttccagta ccagatcatc atgaccatga tcgtccataa gaactgggtg gacctggcct 961 gggccgtcag ctactacatc cggttcttca tcacctacat ccctttctac ggcatcctgg 1021 gagccctcct tttcctcaac ttcatcaggt tcctGGAGAG CCACTGGTTT GTGTGGGTCA 1081 CACAGATGAA TCACATCGTC ATGGAGATTG ACCAGGAGGC CTACCGTGAC TGGTTCAGTA 1141 GCCAGCTGAC AGCCACCTGC AACGTGGAGC AGTCCTTCTT CAACGACTGG TTCAGTGGAC 1201 ACCTTAACTT CCAGATTGAG CACCACCTCT TCCCCACCAT GCCCCGGCAC AACTTACACA 1261 AGATCGCCCC GCTGGTGAAG TCTCTATGTG CCAAGCATGG CATTGAATAC CAGGAGAAGC 1321 CGCTACTGAG GGCCCTGCTG GACATCATCA GGTCCCTGAA GAAGTCTGGG AAGCTGTGGC 1381 TGGACGCCTA CCTTCACAAA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1441 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1501 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTTA AGTTCGACAG 1561 AAATATTGGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt