Transcript: Human NM_004265.4

Homo sapiens fatty acid desaturase 2 (FADS2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-27
Taxon:
Homo sapiens (human)
Gene:
FADS2 (9415)
Length:
3091
CDS:
92..1426

Additional Resources:

NCBI RefSeq record:
NM_004265.4
NBCI Gene record:
FADS2 (9415)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004265.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000064755 CCACGGCAAGAACTCAAAGAT pLKO.1 397 CDS 100% 5.625 7.875 N FADS2 n/a
2 TRCN0000291763 CCACGGCAAGAACTCAAAGAT pLKO_005 397 CDS 100% 5.625 7.875 N FADS2 n/a
3 TRCN0000064756 CCTACAATCACCAGCACGAAT pLKO.1 864 CDS 100% 4.950 3.960 N FADS2 n/a
4 TRCN0000291834 CCTACAATCACCAGCACGAAT pLKO_005 864 CDS 100% 4.950 3.960 N FADS2 n/a
5 TRCN0000064757 CCACCTGTCTGTCTACAGAAA pLKO.1 640 CDS 100% 4.950 3.465 N FADS2 n/a
6 TRCN0000291836 CCACCTGTCTGTCTACAGAAA pLKO_005 640 CDS 100% 4.950 3.465 N FADS2 n/a
7 TRCN0000064754 CCGGCACAACTTACACAAGAT pLKO.1 1267 CDS 100% 4.950 3.465 N FADS2 n/a
8 TRCN0000291764 CCGGCACAACTTACACAAGAT pLKO_005 1267 CDS 100% 4.950 3.465 N FADS2 n/a
9 TRCN0000064753 CCGGTTCTTCATCACCTACAT pLKO.1 1003 CDS 100% 4.950 3.465 N FADS2 n/a
10 TRCN0000291835 CCGGTTCTTCATCACCTACAT pLKO_005 1003 CDS 100% 4.950 3.465 N FADS2 n/a
11 TRCN0000114340 GAAGCTGAAATACCTGCCCTA pLKO.1 847 CDS 100% 2.160 1.512 N Fads2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004265.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491819 GTTAAGTTCGACAGAAATATTGGT pLX_317 26.3% 99.9% 99.7% V5 1332_1333insG n/a
2 ccsbBroadEn_11366 pDONR223 100% 86.8% 86.7% None 1158_1332delinsG n/a
3 ccsbBroad304_11366 pLX_304 0% 86.8% 86.7% V5 1158_1332delinsG n/a
4 TRCN0000476133 AGGACCCCAACACACTTATCGGCG pLX_317 19.5% 86.8% 86.7% V5 1158_1332delinsG n/a
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