Construct: ORF TRCN0000491821
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019820.2_s317c1
- DNA Barcode:
- GTCACTCCTGCCCGTATTAATGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EYA4 (2070)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491821
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_172103.3 | 99.8% | 99.8% | 714G>A;1848_1849insG |
| 2 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_001370458.1 | 98.2% | 98% | (many diffs) |
| 3 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_004100.5 | 96.2% | 96% | 209_277del;783G>A;1917_1918insG |
| 4 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010370.2 | 95.6% | 92.8% | (many diffs) |
| 5 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_001301013.1 | 95.4% | 95.2% | (many diffs) |
| 6 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010371.2 | 94.8% | 92% | (many diffs) |
| 7 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_172105.3 | 94.6% | 94.3% | (many diffs) |
| 8 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_001370459.1 | 93.9% | 93.9% | (many diffs) |
| 9 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_005266851.5 | 93.8% | 93.4% | (many diffs) |
| 10 | human | 2070 | EYA4 | EYA transcriptional coactiv... | NM_001301012.1 | 93.1% | 93% | (many diffs) |
| 11 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010369.2 | 93.1% | 90.2% | (many diffs) |
| 12 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010368.2 | 92.2% | 89.4% | (many diffs) |
| 13 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010374.2 | 92.2% | 92.1% | (many diffs) |
| 14 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010373.2 | 91.6% | 88.8% | (many diffs) |
| 15 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XM_017010372.2 | 90.7% | 87.9% | (many diffs) |
| 16 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XR_001743219.2 | 68.9% | (many diffs) | |
| 17 | human | 2070 | EYA4 | EYA transcriptional coactiv... | XR_001743220.2 | 19.4% | (many diffs) | |
| 18 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | NM_010167.5 | 90.6% | 96.4% | (many diffs) |
| 19 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | NM_001347372.1 | 89% | 94.6% | (many diffs) |
| 20 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512523.3 | 89% | 94.6% | (many diffs) |
| 21 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512524.3 | 89% | 94.6% | (many diffs) |
| 22 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_017313799.1 | 87.3% | 92.8% | (many diffs) |
| 23 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_011243112.2 | 87% | 90.9% | (many diffs) |
| 24 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_017313798.1 | 87% | 90.9% | (many diffs) |
| 25 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512526.3 | 86.8% | 90.3% | (many diffs) |
| 26 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512527.3 | 86.4% | 91.2% | (many diffs) |
| 27 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512528.3 | 84.8% | 90.7% | (many diffs) |
| 28 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_011243115.1 | 84.8% | 90.7% | (many diffs) |
| 29 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512529.3 | 84.2% | 90.1% | (many diffs) |
| 30 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XM_006512530.3 | 69.2% | 74.7% | (many diffs) |
| 31 | mouse | 14051 | Eya4 | EYA transcriptional coactiv... | XR_001779457.1 | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1920
- ORF length:
- 1851
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggaagactcc caggatttaa atgaacaatc agtaaagaaa acgtgcacag 121 aatcagatgt ttcacaatct cagaattcca ggtctatgga aatgcaggac ctagcaagtc 181 ctcatactct tgttggaggt ggtgatactc caggtagctc caaactggaa aaatctaatc 241 tcagcagcac atcagttact acaaatggga caggagtgtc tcttcttgca gtcaaaacag 301 agcccttgaa cagcagtgaa accacagcca cgactggaga tggagcgctt gacactttta 361 ctgggtcagt aattacaagt agtggctaca gccccagatc agcacatcag tattccccac 421 agctgtatcc ttccaagccc tatccacaca ttctttctac accagcagct caaacaatgt 481 ctgcctatgc aggccagact cagtattcgg ggatgcagca gccagccgtc tacacagcct 541 actcacagac aggacagccc tacagcttgc ccacttacga tttgggtgtg atgttgccag 601 ccatcaagac agagagtgga ctttcccaaa ctcagtcccc attacagagt ggctgcctca 661 gttacagccc agggttctct accccacagc caggccagac accttattct taccaaatgc 721 caggttctag ttttgcacca tcatctacta tttatgcaaa taattcagtt tccaattcaa 781 caaatttcag tggttcacaa caggattatc catcctatac agcctttggc caaaaccagt 841 atgcacagta ttattcagca tcaacgtatg gagcgtatat gacatcgaat aacacagccg 901 atggcacacc ctcttcaacc tctacttatc agttgcagga atctctccca ggactgacta 961 accaaccagg agagttcgat accatgcaga gtccctccac acccatcaaa gatcttgatg 1021 agagaacctg taggagttct gggtcaaagt ccagaggaag aggccggaaa aataatccct 1081 ccccgcctcc tgatagtgac ctggagcgtg tgtttgtctg ggatttggat gaaaccatca 1141 ttgtttttca ctcactgctc accgggtctt atgcacagaa gtatggcaag gatcccccca 1201 tggctgtaac ccttggactc cgcatggaag aaatgatttt taatcttgct gatactcatt 1261 tgttttttaa tgatttagag gagtgtgatc aagttcatat agatgatgtt tcctctgatg 1321 ataatgggca ggacttaagt acctacagtt ttgcaactga tggcttccat gcagctgcaa 1381 gtagtgcaaa cctttgtttg ccaacaggtg taagaggagg ggttgactgg atgaggaagt 1441 tggcttttcg ttacagaaga gtaaaagaat tatataacac ctacaagaac aacgttggag 1501 gactccttgg ccctgccaag agggatgcct ggctacagtt aagggcagag attgaaggTC 1561 TGACAGATTC CTGGCTAACA AATGCACTTA AGTCTTTATC AATTATTAGC ACTAGGAGTA 1621 ACTGCATAAA TGTCTTGGTA ACGACAACTC AACTGATCCC AGCACTTGCG AAGGTTCTAC 1681 TCTATAGTTT AGGAGGTGCT TTCCCCATTG AGAATATTTA CAGTGCAACT AAAATAGGAA 1741 AAGAAAGTTG CTTTGAACGA ATAATGCAAA GGTTTGGCAG AAAAGTAGTG TATGTTGTAA 1801 TTGGGGATGG TGTAGAAGAA GAACAGGCAG CAAAAAAGCA CAACATGCCC TTCTGGAGGA 1861 TATCCAGTCA CTCAGACCTC CTGGCTCTCC ACCAAGCACT GGAATTAGAG TATTTGGACC 1921 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1981 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2041 ATATATCTTG TGGAAAGGAC GAGTCACTCC TGCCCGTATT AATGCAACGC GTTAAGTCga 2101 caatcaacct ctggattaca aaatttgtga aagatt