Transcript: Mouse XM_006512527.3

PREDICTED: Mus musculus EYA transcriptional coactivator and phosphatase 4 (Eya4), transcript variant X7, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Eya4 (14051)
Length:
5752
CDS:
704..2506

Additional Resources:

NCBI RefSeq record:
XM_006512527.3
NBCI Gene record:
Eya4 (14051)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006512527.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000440950 CAGTCCTATCTAGACCTATAA pLKO_005 2985 3UTR 100% 13.200 18.480 N Eya4 n/a
2 TRCN0000097801 GCTCAAACAATGTCGGCCTAT pLKO.1 1055 CDS 100% 4.050 5.670 N Eya4 n/a
3 TRCN0000441814 GGCTTCTTCTACACATATTAA pLKO_005 2583 3UTR 100% 15.000 12.000 N Eya4 n/a
4 TRCN0000446738 GAAGCTCTATGGTCTTATTAT pLKO_005 2697 3UTR 100% 15.000 10.500 N Eya4 n/a
5 TRCN0000454509 ATGTTTCCTCCGACGACAATG pLKO_005 1893 CDS 100% 10.800 7.560 N Eya4 n/a
6 TRCN0000097804 CGGATGGAAGAAATGATCTTT pLKO.1 1808 CDS 100% 5.625 3.938 N Eya4 n/a
7 TRCN0000097802 GCCTAACAGATTCCTGGCTAA pLKO.1 2145 CDS 100% 4.050 2.835 N Eya4 n/a
8 TRCN0000097800 GCCATGTTCATATCAACATAT pLKO.1 3578 3UTR 100% 1.320 0.924 N Eya4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006512527.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06177 pDONR223 100% 86.5% 91.4% None (many diffs) n/a
2 ccsbBroad304_06177 pLX_304 0% 86.5% 91.4% V5 (many diffs) n/a
3 TRCN0000492103 TGTGGATTTAGGAAACATGCTTAT pLX_317 19.1% 86.5% 91.4% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000491821 GTCACTCCTGCCCGTATTAATGCA pLX_317 19.7% 86.4% 91.2% V5 (many diffs) n/a
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