Construct: ORF TRCN0000491827

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001703.1_s317c1
Derived from:
ccsbBroadEn_08805
DNA Barcode:
CGCAGGAATATCAATACTACTTAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC22A23 (63027)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491827
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 63027 SLC22A23 solute carrier family 22 me... NM_001286455.1 99.9% 100% 678A>G
2 human 63027 SLC22A23 solute carrier family 22 me... NM_021945.6 99.9% 100% 678A>G
3 human 63027 SLC22A23 solute carrier family 22 me... XM_017011181.1 99.9% 100% 678A>G
4 human 63027 SLC22A23 solute carrier family 22 me... XM_017011186.1 99.9% 100% 678A>G
5 human 63027 SLC22A23 solute carrier family 22 me... XM_017011182.1 95.1% 94.3% (many diffs)
6 human 63027 SLC22A23 solute carrier family 22 me... XM_017011184.1 91.5% 91.6% 0_1ins102;576A>G
7 human 63027 SLC22A23 solute carrier family 22 me... XM_017011180.1 79% 79.1% 1_321del;999A>G
8 human 63027 SLC22A23 solute carrier family 22 me... NM_015482.1 58.9% 59% 1_843del;1521A>G
9 human 63027 SLC22A23 solute carrier family 22 me... XM_011514801.2 56.9% 57% 1_915del;1593A>G
10 human 63027 SLC22A23 solute carrier family 22 me... NR_104448.1 22.4% (many diffs)
11 mouse 73102 Slc22a23 solute carrier family 22, m... XM_006516794.3 62.7% 67.2% (many diffs)
12 mouse 73102 Slc22a23 solute carrier family 22, m... XM_006516793.3 52.9% 56.6% (many diffs)
13 mouse 73102 Slc22a23 solute carrier family 22, m... NM_001033167.3 52.2% 56% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1284
ORF length:
1215
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gttcagcaca ctcaggttct ttgaaggatt ttgcctggct ggaatcattc
121tcaccttgta tgctttacga atagagctgt gcccccctgg aaaacggttc atgattacga
181tggtggcgag cttcgtggcc atggcgggcc agttcctcat gcctgggcta gccgccctgt
241gccgggattg gcaggtgctg caggccctca tcatctgccc cttcctgctc atgctgctct
301actggtcgat attccccgag tccctccggt ggctaatggc cacccagcag tttgagtctg
361caaagaggct gatcctccac ttcacacaga agaatcgcat gaaccctgag ggcgacatca
421agggtgtgat accagagctg gagaaagagc tttcccggag gcccaagaag gtctgcatcg
481tgaaggtggt ggggacacgg aacctgtgga agaacattgt ggtcctgtgt gtgaactcgc
541tgacggggta cgggatccac cactgctttg ccaggagcat gatgggccac gaggtgaagg
601tgccgctcct ggagaacttc tatgctgact actataccac ggccagcatc gcgctggtgt
661cctgcctggc catgtgcgtg gtggtccgat tcctcgggcg caggggaggg ctgctgctct
721tcatgatcct caccgccctg gcctcgctcc tgcagctcgg cctcctcaac ctgattggaa
781agtacagcca gcacccagac tcagggatga gtgacagcgt caaggacaaa ttttccatcg
841cgttttccat cgtgggcatg tttgcctccc atgcggtggg gagcctcagc gtgttcttct
901gtgcggagat caccccgacg gtgataaggt gtggcgggct ggggctggtg ctggccagcg
961cgggcttcgg catgctgacg gcacccatca tcgagctgca caaccagaaa ggctacttcc
1021tgcaccacat catctttgcc TGCTGCACGC TCATCTGCAT CATCTGCATC CTCCTGCTGC
1081CCGAGAGCAG GGACCAGAAC CTGCCTGAGA ACATTTCTAA CGGGGAGCAC TACACGCGCC
1141AGCCGCTGCT GCCGCACAAG AAGGGGGAGC AGCCACTGCT GCTCACCAAC GCCGAGCTCA
1201AGGACTACTC GGGCCTCCAC GATGCCGCAG CCGCGGGTGA CACACTGCCC GAGGGTGCCA
1261CGGCCAACGG CATGAAGGCC ATGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1321AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1381TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GCAGGAATAT
1441CAATACTACT TAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1501att

Download FASTA (ORF) (Full)