Construct: ORF TRCN0000491827
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001703.1_s317c1
- Derived from:
- ccsbBroadEn_08805
- DNA Barcode:
- CGCAGGAATATCAATACTACTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC22A23 (63027)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491827
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63027 | SLC22A23 | solute carrier family 22 me... | NM_001286455.1 | 99.9% | 100% | 678A>G |
| 2 | human | 63027 | SLC22A23 | solute carrier family 22 me... | NM_021945.6 | 99.9% | 100% | 678A>G |
| 3 | human | 63027 | SLC22A23 | solute carrier family 22 me... | XM_017011181.1 | 99.9% | 100% | 678A>G |
| 4 | human | 63027 | SLC22A23 | solute carrier family 22 me... | XM_017011186.1 | 99.9% | 100% | 678A>G |
| 5 | human | 63027 | SLC22A23 | solute carrier family 22 me... | XM_017011182.1 | 95.1% | 94.3% | (many diffs) |
| 6 | human | 63027 | SLC22A23 | solute carrier family 22 me... | XM_017011184.1 | 91.5% | 91.6% | 0_1ins102;576A>G |
| 7 | human | 63027 | SLC22A23 | solute carrier family 22 me... | XM_017011180.1 | 79% | 79.1% | 1_321del;999A>G |
| 8 | human | 63027 | SLC22A23 | solute carrier family 22 me... | NM_015482.1 | 58.9% | 59% | 1_843del;1521A>G |
| 9 | human | 63027 | SLC22A23 | solute carrier family 22 me... | XM_011514801.2 | 56.9% | 57% | 1_915del;1593A>G |
| 10 | human | 63027 | SLC22A23 | solute carrier family 22 me... | NR_104448.1 | 22.4% | (many diffs) | |
| 11 | mouse | 73102 | Slc22a23 | solute carrier family 22, m... | XM_006516794.3 | 62.7% | 67.2% | (many diffs) |
| 12 | mouse | 73102 | Slc22a23 | solute carrier family 22, m... | XM_006516793.3 | 52.9% | 56.6% | (many diffs) |
| 13 | mouse | 73102 | Slc22a23 | solute carrier family 22, m... | NM_001033167.3 | 52.2% | 56% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1284
- ORF length:
- 1215
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttcagcaca ctcaggttct ttgaaggatt ttgcctggct ggaatcattc 121 tcaccttgta tgctttacga atagagctgt gcccccctgg aaaacggttc atgattacga 181 tggtggcgag cttcgtggcc atggcgggcc agttcctcat gcctgggcta gccgccctgt 241 gccgggattg gcaggtgctg caggccctca tcatctgccc cttcctgctc atgctgctct 301 actggtcgat attccccgag tccctccggt ggctaatggc cacccagcag tttgagtctg 361 caaagaggct gatcctccac ttcacacaga agaatcgcat gaaccctgag ggcgacatca 421 agggtgtgat accagagctg gagaaagagc tttcccggag gcccaagaag gtctgcatcg 481 tgaaggtggt ggggacacgg aacctgtgga agaacattgt ggtcctgtgt gtgaactcgc 541 tgacggggta cgggatccac cactgctttg ccaggagcat gatgggccac gaggtgaagg 601 tgccgctcct ggagaacttc tatgctgact actataccac ggccagcatc gcgctggtgt 661 cctgcctggc catgtgcgtg gtggtccgat tcctcgggcg caggggaggg ctgctgctct 721 tcatgatcct caccgccctg gcctcgctcc tgcagctcgg cctcctcaac ctgattggaa 781 agtacagcca gcacccagac tcagggatga gtgacagcgt caaggacaaa ttttccatcg 841 cgttttccat cgtgggcatg tttgcctccc atgcggtggg gagcctcagc gtgttcttct 901 gtgcggagat caccccgacg gtgataaggt gtggcgggct ggggctggtg ctggccagcg 961 cgggcttcgg catgctgacg gcacccatca tcgagctgca caaccagaaa ggctacttcc 1021 tgcaccacat catctttgcc TGCTGCACGC TCATCTGCAT CATCTGCATC CTCCTGCTGC 1081 CCGAGAGCAG GGACCAGAAC CTGCCTGAGA ACATTTCTAA CGGGGAGCAC TACACGCGCC 1141 AGCCGCTGCT GCCGCACAAG AAGGGGGAGC AGCCACTGCT GCTCACCAAC GCCGAGCTCA 1201 AGGACTACTC GGGCCTCCAC GATGCCGCAG CCGCGGGTGA CACACTGCCC GAGGGTGCCA 1261 CGGCCAACGG CATGAAGGCC ATGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1321 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1381 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GCAGGAATAT 1441 CAATACTACT TAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1501 att