Transcript: Human NR_104448.1

Homo sapiens solute carrier family 22 member 23 (SLC22A23), transcript variant 5, non-coding RNA.

Source:
NCBI, updated 2018-05-24
Taxon:
Homo sapiens (human)
Gene:
SLC22A23 (63027)
Length:
5416
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_104448.1
NBCI Gene record:
SLC22A23 (63027)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_104448.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000059586 CCCTGTCAGCCTTTCAATAAA pLKO.1 4226 3UTR 100% 15.000 21.000 N SLC22A23 n/a
2 TRCN0000427211 GACAGCGTCAAGGACAAATTT pLKO_005 1182 3UTR 100% 15.000 10.500 N SLC22A23 n/a
3 TRCN0000252940 TAATCTTTGGCTACCTAATAA pLKO_005 260 3UTR 100% 15.000 10.500 N Slc22a23 n/a
4 TRCN0000059585 GCTGTTGAGCAACACCTTTAT pLKO.1 3911 3UTR 100% 13.200 9.240 N SLC22A23 n/a
5 TRCN0000059587 GCTGTTCCCTTCCTGGCTAAT pLKO.1 4114 3UTR 100% 10.800 7.560 N SLC22A23 n/a
6 TRCN0000427343 TTTCCATCGTGGGCATGTTTG pLKO_005 1213 3UTR 100% 10.800 7.560 N SLC22A23 n/a
7 TRCN0000059583 GCAACACCTTTATGAAATGTT pLKO.1 3919 3UTR 100% 5.625 3.938 N SLC22A23 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_104448.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15959 pDONR223 0% 22.4% None (many diffs) n/a
2 ccsbBroad304_15959 pLX_304 0% 22.4% V5 (many diffs) n/a
3 TRCN0000480955 TAGGGCTAGACCTGTCATTTAATA pLX_317 33% 22.4% V5 (many diffs) n/a
4 ccsbBroadEn_08805 pDONR223 100% 22.4% None (many diffs) n/a
5 ccsbBroad304_08805 pLX_304 0% 22.4% V5 (many diffs) n/a
6 TRCN0000491827 CGCAGGAATATCAATACTACTTAA pLX_317 20.5% 22.4% V5 (many diffs) n/a
7 ccsbBroadEn_12430 pDONR223 100% 8.4% None 1_3802del;4097C>G;4262_5416del n/a
8 ccsbBroad304_12430 pLX_304 0% 8.4% V5 1_3802del;4097C>G;4262_5416del n/a
9 TRCN0000474449 TGCTCTAATACTGGCAAAATACCC pLX_317 22.1% 8.4% V5 1_3802del;4097C>G;4262_5416del n/a
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