Construct: ORF TRCN0000491837

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020784.2_s317c1
DNA Barcode:
TTTAGGACGTCGCGTTATCACGGT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
CHRM3 (1131)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491837
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1131 CHRM3 cholinergic receptor muscar... NM_000740.3 100% 100%
2 human 1131 CHRM3 cholinergic receptor muscar... NM_001347716.2 100% 100%
3 human 1131 CHRM3 cholinergic receptor muscar... XM_005273032.3 100% 100%
4 human 1131 CHRM3 cholinergic receptor muscar... XM_011544041.2 100% 100%
5 human 1131 CHRM3 cholinergic receptor muscar... XM_011544043.2 100% 100%
6 human 1131 CHRM3 cholinergic receptor muscar... XM_011544044.2 100% 100%
7 human 1131 CHRM3 cholinergic receptor muscar... XM_011544046.2 100% 100%
8 human 1131 CHRM3 cholinergic receptor muscar... XM_011544047.2 100% 100%
9 human 1131 CHRM3 cholinergic receptor muscar... XM_017000152.2 100% 100%
10 human 1131 CHRM3 cholinergic receptor muscar... XM_017000153.1 100% 100%
11 human 1131 CHRM3 cholinergic receptor muscar... XM_017000154.1 100% 100%
12 human 1131 CHRM3 cholinergic receptor muscar... XM_017000155.1 100% 100%
13 human 1131 CHRM3 cholinergic receptor muscar... XM_017000156.1 100% 100%
14 human 1131 CHRM3 cholinergic receptor muscar... XM_017000157.2 100% 100%
15 human 1131 CHRM3 cholinergic receptor muscar... XM_017000158.1 100% 100%
16 human 1131 CHRM3 cholinergic receptor muscar... XM_017000159.1 100% 100%
17 human 1131 CHRM3 cholinergic receptor muscar... XM_017000160.2 100% 100%
18 human 1131 CHRM3 cholinergic receptor muscar... XM_017000161.1 100% 100%
19 human 1131 CHRM3 cholinergic receptor muscar... XM_017000162.1 100% 100%
20 human 1131 CHRM3 cholinergic receptor muscar... XM_017000163.2 100% 100%
21 mouse 12671 Chrm3 cholinergic receptor, musca... NM_033269.4 86.9% 91.6% (many diffs)
22 mouse 12671 Chrm3 cholinergic receptor, musca... XM_006516468.3 86.9% 91.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1842
ORF length:
1770
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaccttg cacaataaca gtacaacctc gcctttgttt ccaaacatca
121gctcctcctg gatacacagc ccctccgatg cagggctgcc cccgggaacc gtcactcatt
181tcggcagcta caatgtttct cgagcagctg gcaatttctc ctctccagac ggtaccaccg
241atgaccctct gggaggtcat accgtctggc aagtggtctt catcgctttc ttaacgggca
301tcctggcctt ggtgaccatc atcggcaaca tcctggtaat tgtgtcattt aaggtcaaca
361agcagctgaa gacggtcaac aactacttcc tcttaagcct ggcctgtgcc gatctgatta
421tcggggtcat ttcaatgaat ctgtttacga cctacatcat catgaatcga tgggccttag
481ggaacttggc ctgtgacctc tggcttgcca ttgactacgt agccagcaat gcctctgtta
541tgaatcttct ggtcatcagc tttgacagat acttttccat cacgaggccg ctcacgtacc
601gagccaaacg aacaacaaag agagccggtg tgatgatcgg tctggcttgg gtcatctcct
661ttgtcctttg ggctcctgcc atcttgttct ggcaatactt tgttggaaag agaactgtgc
721ctccgggaga gtgcttcatt cagttcctca gtgagcccac cattactttt ggcacagcca
781tcgctgcttt ttatatgcct gtcaccatta tgactatttt atactggagg atctataagg
841aaactgaaaa gcgtaccaaa gagcttgctg gcctgcaagc ctctgggaca gaggcagaga
901cagaaaactt tgtccacccc acgggcagtt ctcgaagctg cagcagttac gaacttcaac
961agcaaagcat gaaacgctcc aacaggagga agtatggccg ctgccacttc tggttcacaa
1021ccaagagctg gaaacccagc tccgagcaga tggaccaaga ccacagcagc agtgacagtt
1081ggaacaacaa tgatgctgct gcctccctgg agaactccgc ctcctccgac gaggaggaca
1141ttggctccga gacgagagcc atctactcca tcgtgctcaa gcttccgggt cacagcacca
1201tcctcaactc caccaagtta ccctcatcgg acaacctgca ggtgcctgag gaggagctgg
1261ggatggtgga cttggagagg aaagccgaca agctgcaggc ccagaagagc gtggacgatg
1321gaggcagttt tccaaaaagc ttctccaagc ttcccatcca gctagagtca gccgtggaca
1381cagctaagac ttctgacgtc aactcctcag tgggtaagag cacggccact ctacctctgt
1441ccTTCAAGGA AGCCACTCTG GCCAAGAGGT TTGCTCTGAA GACCAGAAGT CAGATCACTA
1501AGCGGAAAAG GATGTCCCTG GTCAAGGAGA AGAAAGCGGC CCAGACCCTC AGTGCGATCT
1561TGCTTGCCTT CATCATCACT TGGACCCCAT ACAACATCAT GGTTCTGGTG AACACCTTTT
1621GTGACAGCTG CATACCCAAA ACCTTTTGGA ATCTGGGCTA CTGGCTGTGC TACATCAACA
1681GCACCGTGAA CCCCGTGTGC TATGCTCTGT GCAACAAAAC ATTCAGAACC ACTTTCAAGA
1741TGCTGCTGCT GTGCCAGTGT GACAAAAAAA AGAGGCGCAA GCAGCAGTAC CAGCAGAGAC
1801AGTCGGTCAT TTTTCACAAG CGCGCACCCG AGCAGGCCTT GTAGGACCCA GCTTTCTTGT
1861ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1921AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1981GAAAGGACGA TTTAGGACGT CGCGTTATCA CGGTACGCGT TAAGTCgaca atcaacctct
2041ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)