Construct: ORF TRCN0000491860
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021467.2_s317c1
- DNA Barcode:
- TCACAAACTTACCCTGTAGTGGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DIP2C (22982)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491860
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 22982 | DIP2C | disco interacting protein 2... | NM_014974.3 | 99.9% | 100% | 1248G>A |
2 | human | 22982 | DIP2C | disco interacting protein 2... | XM_005252427.4 | 99.3% | 99.3% | 1248G>A;3921_3950del |
3 | human | 22982 | DIP2C | disco interacting protein 2... | XM_011519431.2 | 98.6% | 97.1% | (many diffs) |
4 | human | 22982 | DIP2C | disco interacting protein 2... | XM_011519430.2 | 98% | 96.5% | (many diffs) |
5 | human | 22982 | DIP2C | disco interacting protein 2... | XM_005252426.3 | 96.5% | 96.4% | 158_325del;1416G>A |
6 | human | 22982 | DIP2C | disco interacting protein 2... | XM_005252428.4 | 95.9% | 95.8% | 158_325del;1416G>A;4089_4118del |
7 | human | 22982 | DIP2C | disco interacting protein 2... | XM_011519429.3 | 95.2% | 93.7% | (many diffs) |
8 | human | 22982 | DIP2C | disco interacting protein 2... | XM_011519428.3 | 94.6% | 93.1% | (many diffs) |
9 | human | 22982 | DIP2C | disco interacting protein 2... | XM_011519432.2 | 92.5% | 92.5% | 0_1ins321;927G>A;3600_3629del |
10 | human | 22982 | DIP2C | disco interacting protein 2... | XM_005252430.3 | 62% | 58.6% | (many diffs) |
11 | human | 22982 | DIP2C | disco interacting protein 2... | XM_017015977.1 | 61.6% | 58.3% | (many diffs) |
12 | human | 22982 | DIP2C | disco interacting protein 2... | XM_011519433.3 | 56% | 56% | 0_1ins2037;1884_1913del |
13 | mouse | 208440 | Dip2c | disco interacting protein 2... | NM_001081426.2 | 87.1% | 99.2% | (many diffs) |
14 | mouse | 208440 | Dip2c | disco interacting protein 2... | XM_006516475.3 | 86.9% | 99% | (many diffs) |
15 | mouse | 208440 | Dip2c | disco interacting protein 2... | XM_006516474.2 | 84.2% | 95.7% | (many diffs) |
16 | mouse | 208440 | Dip2c | disco interacting protein 2... | XM_006516473.1 | 83.9% | 95.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 4734
- ORF length:
- 4668
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat 61 tgaccatggc ggaccgcagc ctggagggca tggcgctgcc cctggaggtg cgggcgcgcc 121 tggccgagct ggagctggag ctgtcggaag gtgacatcac acaaaaagga tatgaaaaga 181 agaggtcaaa gttaattgga gcctaccttc cgcagcctcc gagggtggac caagctttgc 241 cgcaagaacg ccgggctcct gtcactcctt cctccgcctc tcgctaccac cgccgacggt 301 cttcagggtc acgagatgag cgctatcggt cagacgtcca cacggaagct gtccaggcgg 361 ctctggccaa acacaaagag cggaagatgg cagtgcctat gccttccaaa cgcaggtccc 421 tggtcgtgca gacctcgatg gacgcctaca cccctccaga tacctcttct ggctcagaag 481 atgaaggctc agtgcagggg gactcccagg gcacccccac ctccagccag ggcagcatca 541 atatggagca ctggatcagc caggccatcc acggctccac cacgtccacc acctcctcgt 601 cctctacgca gagcgggggc agcggggctg cccacaggct ggcggacgtc atggctcaga 661 cccacataga aaatcattct gcacctcctg acgtaaccac gtacacctca gagcactcga 721 tacaggtgga gagaccgcag ggttccacgg ggtcccggac agcgcccaag tacggcaacg 781 ccgagctcat ggagaccggg gatggagtac cagtaagtag ccgggtgtca gcaaaaatcc 841 agcagcttgt caataccctc aaacgaccga aacgaccacc tttacgagaa ttctttgtcg 901 atgactttga agaattatta gaagttcaac aaccggatcc gaaccaacca aagccggagg 961 gggcccagat gctggccatg cgcggagagc agctgggcgt ggtcacgaac tggccgccgt 1021 cgctggaggc cgcactgcag aggtggggca ccatctcgcc caaggcgccc tgcctgacca 1081 ccatggacac caacgggaag cccctctaca tcctcactta cggcaagctg tggacaagaa 1141 gtatgaaggt cgcttacagc attctacaca aattaggcac aaagcaggaa cccatggtcc 1201 ggcctggaga tagggtggca ctggtgttcc ccaacaatga tccggctgcc ttcatggcgg 1261 ctttctacgg ctgcctgctg gccgaggtgg tccccgtgcc catcgaggtg ccactcacca 1321 ggaaggacgc agggagccag cagataggtt tcttgcttgg aagctgtgga gttactgtag 1381 ccttgactag tgacgcctgc cataaaggac ttccaaaaag cccaacggga gagatcccac 1441 agtttaaagg ttggccaaag ctgctgtggt ttgtcacaga gtctaaacat ctctccaaac 1501 cgccccgaga ctggttccca cacattaaag atgccaataa cgacactgcg tatattgagt 1561 acaagacgtg taaggatggc agtgtgctgg gtgtgacggt gacgaggact gcgctgctga 1621 cacactgcca ggccctgacg caggcgtgtg gctacacgga agctgaaacc attgtgaatg 1681 tgctggattt caagaaggac gtcgggctct ggcatggcat cctgacaagc gtcatgaaca 1741 tgatgcatgt gatcagcatc ccgtactcgc tgatgaaggt gaaccctctc tcctggatcc 1801 agaaggtctg ccagtacaaa gcaaaagtgg cgtgtgtgaa atcgagggat atgcattggg 1861 cattagtagc acacagagat cagagagaca tcaacctctc ctctctgcga atgctgatag 1921 tggcggacgg cgcgaacccc tggtctattt cttcttgcga tgcatttctc aatgtcttcc 1981 aaagtaaagg ccttcgacag gaggtcatct gtccttgtgc cagctcgcca gaggccctca 2041 ctgtggccat ccggaggccc acggatgaca gtaaccagcc cccgggccgg ggtgtcctct 2101 ccatgcatgg actgacctat ggggtcattc gtgtggactc ggaagagaag ctgtccgtgc 2161 tcaccgtgca ggatgtcggc ctcgtgatgc ctggagccat catgtgttca gtgaagccag 2221 acggggttcc tcagctgtgc agaacggatg agatcgggga gctgtgtgtg tgtgcagttg 2281 cgacgggcac gtcctactat ggcctctctg gcatgaccaa gaacaccttt gaggtgtttc 2341 ccatgacaag ctccggggct ccgatcagtg aatacccatt cataaggaca ggcttgctgg 2401 ggttcgtggg tcccggaggc ctcgtcttcg tggtgggcaa gatggatggc ctcatggtgg 2461 tcagcgggcg caggcacaac gccgacgaca tcgtggccac tgcgctggcc gtagaaccca 2521 tgaagtttgt ctaccgggga aggatagccg tgttctcggt gaccgtgctg cacgacgaga 2581 ggatcgtgat cgtggctgag cagaggcctg actccacgga agaggacagt ttccagtgga 2641 tgagccgtgt gctgcaggcg attgacagta tacatcaagt tggagtttat tgcctggcct 2701 tggtgccagc aaacaccctc cccaaaaccc cgcttggtgg gatccattta tcagaaacaa 2761 aacagctttt tctggagggc tctctgcacc cctgcaatgt cctaatgtgc ccccacacct 2821 gcgtcacaaa cttgcctaag cctcgacaga agcagccaga aatcggccct gcctctgtga 2881 tggtggggaa cctggtctct gggaagagaa tcgcccaggc cagtggcaga gacctgggtc 2941 agatcgaaga taacgaccag gcacgcaagt tcctgttcct ctcagaggtc ttgcagtgga 3001 gagcacagac caccccggac cacatcctct acacgctgct caactgtcgg ggtgcgatag 3061 cgaactcgct gacctgcgtg cagctgcaca agagagctga gaagatcgcc gtgatgctga 3121 tggagagggg ccaccttcag gacggcgacc acgtggcctt ggtctacccc ccaggaatag 3181 acctgatagc agcgttttat ggttgcctgt acgcaggctg tgtgccaata accgtccgtc 3241 ccccgcaccc acagaacatc gcgacgacgt tgcctaccgt caagatgatt gtggaggtga 3301 gtcgctctgc ctgtctgatg acgacacagc tgatctgtaa gttgctgcgg tccagggagg 3361 cggcggcggc tgtggacgtc aggacgtggc ccctcatcct ggacacagat gatttgccaa 3421 agaagcggcc tgcccagatc tgcaaacctt gcaacccaga cactcttgca tatctcgact 3481 tcagcgtgtc cacaactggg atgctagctg gcgtaaagat gtctcacgca gccaccagtg 3541 ccttctgccg ttccattaag ctgcagtgtg aactttaccc ctctagagaa gtggccatct 3601 gcctggaccc ttactgtgga ctgggatttg tcctctggtg cctctgcagt gtgtattctg 3661 ggcaccagtc catcctgatc ccgccctctg agctggaaac caaccccgcc ttgtggcttc 3721 ttgccgtgag tcagtacaaa gtccgagaca cgttttgctc ctactccgtg atggagctgt 3781 gcaccaaggg gctgggctcg caaacagagt ccctcaaggc gcgagggctg gacttgtccc 3841 gagtgaggac ctgcgtggtt gtggcggaag agaggcctcg gatcgcactc acacagtcgt 3901 tctcaaagct gtttaaggac ctgggccttc acccgcgggc cgtcagcacc tcgttcggtt 3961 gcagggtgaa cctggcgatt tgcttgcagg gaacctcagg acctgaccca accactgtct 4021 acgtggacat gagagccctg agacacgaca gagtccgctt agtggaaaga ggatcccctc 4081 atagtctgcc cctgatggaa tcgggaaaga tacttccagg ggttcggatt ataattgcca 4141 acccagaaac aaaaggaccg ctgggggact cacaccttgg agagatttgg gttcacagtg 4201 cccacaatgc cagcggttat ttcactattt acggagacga atccctccag tcagatcact 4261 tcaactcaag actaagtttt ggagacaccc agaccatctg ggcacgcaca ggctacttgg 4321 ggttcctgcg gagaactgag ctcacagatg caaatggaga gcgccatgat gccctctacg 4381 tggtaggggc actggacgaa gccatGGAGC TGCGGGGCAT GCGGTACCAC CCAATCGACA 4441 TTGAGACCTC GGTCATCAGA GCCCATAAAA GCGTTACGGA ATGTGCTGTG TTTACCTGGA 4501 CAAATTTGTT GGTGGTTGTG GTTGAGCTGG ATGGGTCGGA ACAAGAAGCC TTGGACCTGG 4561 TTCCCTTGGT GACCAACGTG GTCCTGGAGG AGCACTACCT GATCGTCGGA GTGGTGGTCG 4621 TGGTGGACAT CGGCGTCATC CCCATCAACT CCCGTGGGGA GAAGCAGCGC ATGCACCTGC 4681 GAGACGGGTT TTTGGCAGAC CAGCTAGACC CCATCTATGT GGCCTACAAC ATGTAGCAAT 4741 TGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 4801 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 4861 GGCTTTATAT ATCTTGTGGA AAGGACGATC ACAAACTTAC CCTGTAGTGG TGACGCGTTA 4921 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt