Construct: ORF TRCN0000491881
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015754.2_s317c1
- Derived from:
- ccsbBroadEn_04001
- DNA Barcode:
- ATGTTTATACCCAGTGGGACCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF747 (65988)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491881
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65988 | ZNF747 | zinc finger protein 747 | NM_023931.3 | 100% | 100% | |
| 2 | human | 65988 | ZNF747 | zinc finger protein 747 | NM_001305020.1 | 99.4% | 99.4% | 480_481insCAG |
| 3 | human | 65988 | ZNF747 | zinc finger protein 747 | NM_001305018.1 | 38.2% | 30.6% | 228_229ins140;434_993del |
| 4 | human | 65988 | ZNF747 | zinc finger protein 747 | NM_001305019.1 | 37.9% | 31.2% | 228_229ins140;340_341insCAG;431_990del |
| 5 | human | 92595 | ZNF764 | zinc finger protein 764 | NM_033410.4 | 26.9% | 20.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 639
- ORF length:
- 573
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cgatccgtcg ctggggctga cagtccccat ggcgccgcct ctggccccgc 121 tccctccccg ggacccaaac ggggcgggat ccgagtggag aaagcccggg gccgtgagct 181 tcgccgacgt ggccgtgtac ttctcccggg aggagtgggg ctgcctgcgg cccgcgcaga 241 gggccctgta ccgggacgtg atgcgggaga cctacggcca cctgggcgcg ctcggtgaga 301 gccccaccTG CTTGCCTGGG CCCTGCGCCT CCACAGGCCC TGCCGCGCCT CTGGGAGCTG 361 CGTGTGGAGT TGGGGGCCCC GGGGCCGGGC AGGCGGCCTC CTCGCAGCGT GGGGTTTGCG 421 TTCTTCTCCC CCAGGAGTCG GAGGCAGCAA GCCGGCGCTC ATCTCCTGGG TGGAGGAGAA 481 GGCCGAACTG TGGGATCCGG CTGCCCAGGA TCCGGAGGTG GCGAAGTGTC CGACAGAAGC 541 GGACCCAGCA GATTCCAGAA ACAAGGAAGA GGAAAGACAA AGGGAAGGGA CGGGAGCCCT 601 GGAGAAGCCC GACCCTGTGG CCGCCGGGTC TCCTGGGCTG CCCAACTTTC TTGTACAAAG 661 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 721 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 781 ACGAATGTTT ATACCCAGTG GGACCTTGAC GCGTTAAGTC gacaatcaac ctctggatta 841 caaaatttgt gaaagatt