Construct: ORF TRCN0000491889
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011300.1_s317c1
- Derived from:
- ccsbBroadEn_13100
- DNA Barcode:
- ACCTCTCTTGGAGTAATAACCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPATA2L (124044)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491889
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124044 | SPATA2L | spermatogenesis associated ... | NM_152339.4 | 34.9% | 33.4% | (many diffs) |
| 2 | human | 124044 | SPATA2L | spermatogenesis associated ... | XM_005256279.5 | 34.9% | 33.4% | (many diffs) |
| 3 | mouse | 78779 | Spata2l | spermatogenesis associated ... | NM_030176.2 | 30.6% | 30.8% | (many diffs) |
| 4 | mouse | 78779 | Spata2l | spermatogenesis associated ... | XM_006531504.3 | 30.6% | 30.8% | (many diffs) |
| 5 | mouse | 78779 | Spata2l | spermatogenesis associated ... | XM_006531505.3 | 30.6% | 30.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 522
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cagcagctcg ctgtccgagg actaccgcca gtgcctagag cgcgagctgc 121 gacggggccg cgcgggcgtg tgcggGGACC CCTCGCTGCG CGCGGTGCTC TGGCAGATCC 181 TGGTGGAGGA CTTCGACCTG CACGGGGCGC TGCAGGACGA CGCGCTGGCT CTGCTCACCG 241 ACGGGCTGTG GGGCCGCGCC GACCTGGCGC CCGCGCTACG CGGCCTGGCT CGCGCCTTCG 301 AGCTTCTGGA GCTCGCCGCG GTGCACCTGT ACCTGCTGCC CTGGAGGAAG GAGTTCACCA 361 CCATCAAGAC CTTCTCTGGG GGCTACGTGC ACGTGCTGAA GGGTGTGCTC TCAGACGACC 421 TCCTCCTGAA GAGCTTCCAG AAGATGGGCT ACGTACGCAG AGACAGCCAT CGGCTCATGC 481 TCTGCTGGAG CCAATCTGCG GGGCTCTGCC GGGTGCACGG CTGCCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGAACC TCTCTTGGAG TAATAACCTT AACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t