Transcript: Mouse XM_006531505.3

PREDICTED: Mus musculus spermatogenesis associated 2-like (Spata2l), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Spata2l (78779)
Length:
2393
CDS:
100..1380

Additional Resources:

NCBI RefSeq record:
XM_006531505.3
NBCI Gene record:
Spata2l (78779)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006531505.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000106349 CCCGACTCATTCACTGCGCAT pLKO.1 1272 CDS 100% 0.720 1.008 N Spata2l n/a
2 TRCN0000414552 GGAACCGCCAGCTGATGATAT pLKO_005 930 CDS 100% 13.200 9.240 N Spata2l n/a
3 TRCN0000428473 GAAAGAGCAGAGCTTACTTAG pLKO_005 1830 3UTR 100% 10.800 7.560 N Spata2l n/a
4 TRCN0000419018 TGAGTCGCTCAGGAGACTTAG pLKO_005 1043 CDS 100% 10.800 7.560 N Spata2l n/a
5 TRCN0000437932 GTTGGAGTGTGAGATCCTAAG pLKO_005 576 CDS 100% 6.000 4.200 N Spata2l n/a
6 TRCN0000106347 CAGATTCTGGTGGAAGACTTT pLKO.1 208 CDS 100% 4.950 3.465 N Spata2l n/a
7 TRCN0000106348 GAGAAAGGAGTTCACCACTAT pLKO.1 378 CDS 100% 4.950 3.465 N Spata2l n/a
8 TRCN0000106345 GCAGCTAACCAGGAAGAAGTT pLKO.1 2054 3UTR 100% 4.950 3.465 N Spata2l n/a
9 TRCN0000106346 CAGGTGGATAACCTGCTCTAT pLKO.1 1336 CDS 100% 0.495 0.347 N Spata2l n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006531505.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09479 pDONR223 99.3% 80.9% 85% None (many diffs) n/a
2 ccsbBroad304_09479 pLX_304 0% 80.9% 85% V5 (many diffs) n/a
3 TRCN0000478410 TGATAGCGGTCTACTATTTCTCCA pLX_317 27.7% 80.9% 85% V5 (many diffs) n/a
4 ccsbBroadEn_13100 pDONR223 100% 30.6% 30.8% None (many diffs) n/a
5 ccsbBroad304_13100 pLX_304 0% 30.6% 30.8% V5 (many diffs) n/a
6 TRCN0000491889 ACCTCTCTTGGAGTAATAACCTTA pLX_317 81.3% 30.6% 30.8% V5 (many diffs) n/a
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