Construct: ORF TRCN0000491891
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020956.2_s317c1
- DNA Barcode:
- TTTTGATCCTCTTGGCGAACTCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- OR2T4 (127074)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491891
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127074 | OR2T4 | olfactory receptor family 2... | NM_001004696.1 | 100% | 100% | |
| 2 | human | 343563 | OR2T29 | olfactory receptor family 2... | NM_001004694.2 | 85.4% | 81.8% | (many diffs) |
| 3 | human | 401993 | OR2T5 | olfactory receptor family 2... | NM_001004697.2 | 85.2% | 81.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1116
- ORF length:
- 1044
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggacaac atcacctgga tggccagcca cactggatgg tcggatttca 121 tcctgatggg actcttcaga caatccaaac atccaatggc caatatcacc tggatggcca 181 accacactgg atggtcggat ttcatcctgt tgggactctt cagacaatcc aaacatccag 241 cactactttg tgtggtcatt tttgtggttt tcctgatggc gttgtctgga aatgctgtcc 301 tgatccttct gatacactgt gacgcccacc tccacacccc catgtacttt ttcatcagtc 361 aattgtctct catggacatg gcgtacattt ctgtcactgt gcccaagatg ctcctggacc 421 aggtcatggg tgtgaataag atctcagccc ctgagtgtgg gatgcagatg ttcttctacg 481 tgacactagc aggttcagaa tttttccttc tagccaccat ggcctatgac cgctacgtgg 541 ccatctgcca tcctctccgt taccctgtcc tcatgaacca tagggtgtgt ctcttcctgt 601 catcaggctg ctggttcctg ggctcagtgg atggcttcac attcactccc atcaccatga 661 ccttcccctt ccgtggatcc cgggagattc atcatttctt ctgtgaagtt cctgctgtat 721 tgaatctctc ctgctcagac acctcactct atgagatttt catgtacttg tgctgtgtcc 781 tcatgctcct catccctgtg gtgatcattt caagctccta tttactcaTC CTCCTCACCA 841 TCCACGGGAT GAACTCAGCA GAGGGCCGGA AAAAGGCCTT TGCCACCTGC TCCTCCCACC 901 TGACTGTGGT CATCCTCTTC TATGGGGCTG CCATCTACAC CTACATGCTC CCCAGCTCCT 961 ACCACACCCC TGAGAAGGAC ATGATGGTAT CTGTCTTCTA TACCATCCTC ACTCCAGTGG 1021 TGAACCCTTT AATCTATAGT CTTAGGAATA AGGATGTCAT GGGGGCTCTG AAGAAAATGT 1081 TAACAGTGGA ACCTGCCTTT CAAAAAGCTA TGGAGTAGAA CCCAGCTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGATTTTGA TCCTCTTGGC GAACTCAAAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt