Construct: ORF TRCN0000491895
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019725.2_s317c1
- DNA Barcode:
- AGCCCTGCGATTTCACCTTTTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK17B (9262)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491895
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9262 | STK17B | serine/threonine kinase 17b | NM_004226.4 | 99.6% | 99.7% | (many diffs) |
| 2 | human | 9262 | STK17B | serine/threonine kinase 17b | XM_011512168.3 | 99.6% | 99.7% | (many diffs) |
| 3 | human | 9262 | STK17B | serine/threonine kinase 17b | XM_011512169.1 | 99.6% | 99.7% | (many diffs) |
| 4 | human | 9262 | STK17B | serine/threonine kinase 17b | XM_011512170.1 | 99.6% | 99.7% | (many diffs) |
| 5 | human | 9262 | STK17B | serine/threonine kinase 17b | XM_011512171.2 | 65.4% | 65.4% | 0_1ins384;357T>C;732_733insG |
| 6 | mouse | 98267 | Stk17b | serine/threonine kinase 17b... | NM_133810.3 | 87.2% | 91.1% | (many diffs) |
| 7 | mouse | 98267 | Stk17b | serine/threonine kinase 17b... | XM_006496359.3 | 58.1% | 60% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1188
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtcgaggagg agatttgatt gccgaagtat ttcaggccta ctaactacaa 121 ctcctcaaat tccaataaaa atggaaaact ttaataattt ctatatactt acatctaaag 181 agctagggag aggtaaattt gctgtggtta gacaatgtat atcaaaatct actggccaag 241 aatatgctgc aaaatttcta aaaaagagaa gaagaggaca ggattgtcgg gcagaaattt 301 tacacgagat tgctgtgctt gaattggcaa agtcttgtcc ccgtgttatt aatcttcatg 361 aggtctatga aaatacaagt gaaatcattt tgatattgga atatgctgca ggtggagaaa 421 ttttcagcct gtgtttacct gagttggctg aaatggtttc tgaaaatgat gttatcagac 481 tcattaaaca aatacttgaa ggagtttatt atctacatca gaataacatt gtacaccttg 541 atttaaagcc acagaatata ttactgagca gcatataccc tctcggggac attaaaatag 601 tagattttgg aatgtctcga aaaatagggc atgcgtgtga acttcgggaa atcatgggaa 661 caccagaata tttagctcca gaaatcctga actatgatcc cattaccaca gcaacagata 721 tgtggaatat tggtataata gcatatatgt tgttaactca cacatcacca tttgtgggag 781 aagataatca agaaacatac ctcaatatct ctcaagttaa tgtagattat tcggaagaaa 841 ctttttcatc agtttcacag ctggccacag actttattca gagcctttta gtaaaaaatc 901 cagagaaaag accaacagca gagatatgcc tttctcattc ttGGCTACAG CAGTGGGACT 961 TTGAAAACTT GTTTCACCCT GAAGAAACTT CCAGTTCCTC TCAAACTCAG GATCATTCTG 1021 TAAGGTCCTC TGAAGACAAG ACTTCTAAAT CCTCCTGTAA TGGAACCTGT GGTGATAGAG 1081 AAGACAAAGA GAATATCCCA GAGGATAGCA GCATGGTTTC CAAAAGATTT CGTTTCGATG 1141 ACTCATTACC CAATCCCCAT GAACTTGTTT CAGATTTGCT CTGTGACCCA GCTTTCTTGT 1201 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1261 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1321 GAAAGGACGA AGCCCTGCGA TTTCACCTTT TCGGACGCGT TAAGTCgaca atcaacctct 1381 ggattacaaa atttgtgaaa gatt