Construct: ORF TRCN0000491911
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000971.1_s317c1
- Derived from:
- ccsbBroadEn_02988
- DNA Barcode:
- TTTATGATCCAAGCAGGATTTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GHITM (27069)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491911
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27069 | GHITM | growth hormone inducible tr... | NM_014394.3 | 100% | 100% | |
| 2 | mouse | 66092 | Ghitm | growth hormone inducible tr... | NM_001199122.1 | 90.4% | 91.6% | (many diffs) |
| 3 | mouse | 66092 | Ghitm | growth hormone inducible tr... | NM_078478.5 | 90.4% | 91.6% | (many diffs) |
| 4 | mouse | 66092 | Ghitm | growth hormone inducible tr... | XM_006519372.3 | 90.4% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1101
- ORF length:
- 1035
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ggctgcaagg ctggtgtgtc tccggacact accttctagg gttttccacc 121 cagctttcac caaggcctcc cctgttgtga agaattccat cacgaagaat caatggctgt 181 taacacctag cagggaatat gccaccaaaa caagaattgg gatccggcgt gggagaactg 241 gccaagaact caaagaggca gcattggaac catcgatgga aaaaatattt aaaattgatc 301 agatgggaag atggtttgtt gctggagggg ctgctgttgg tcttggagca ttgtgctact 361 atggcttggg actgtctaat gagattggag ctattgaaaa ggctgtaatt tggcctcagt 421 atgtcaagga tagaattcat tccacctata tgtacttagc agggagtatt ggtttaacag 481 ctttgtctgc catagcaatc agcagaacgc ctgttctcat gaacttcatg atgagaggct 541 cttgggtgac aattggtgtg acctttgcag ccatggttgg agctggaatg ctggtacgat 601 caataccata tgaccagagc ccaggcccaa agcatcttgc ttggttgcta cattctggtg 661 tgatgggtgc agtggtggct cctctgacaa tattaggggg tcctcttctc atcagagctg 721 catggtacac agctggcatt gtgggaggcc tctccactgt ggccatgtgt gcgcccagtg 781 aaaagtttct gaacatgggt gcacccctgg gagtgggcct gggtctcgtc tttgtgtcct 841 cattgggatc tatgtttctt ccacctacca ccgtggctgg tgccactctt tacTCAGTGG 901 CAATGTACGG TGGATTAGTT CTTTTCAGCA TGTTCCTTCT GTATGATACC CAGAAAGTAA 961 TCAAGCGTGC AGAAGTATCA CCAATGTATG GAGTTCAAAA ATATGATCCC ATTAACTCGA 1021 TGCTGAGTAT CTACATGGAT ACATTAAATA TATTTATGCG AGTTGCAACT ATGCTGGCAA 1081 CTGGAGGCAA CAGAAAGAAA TGGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1141 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1201 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTTA TGATCCAAGC 1261 AGGATTTTTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt