Construct: ORF TRCN0000491934

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008972.1_s317c1
Derived from:
ccsbBroadEn_14792
DNA Barcode:
ATCATCAGAGAAGGCTGTCTTTCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKCH (5583)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491934
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5583 PRKCH protein kinase C eta NM_006255.4 99.9% 100% 393T>C;1674C>T
2 human 5583 PRKCH protein kinase C eta XM_011536954.3 86.3% 83.7% (many diffs)
3 human 5583 PRKCH protein kinase C eta XM_011536955.1 83.2% 82.1% (many diffs)
4 human 5583 PRKCH protein kinase C eta XM_017021458.1 76.3% 76.4% 0_1ins483;1191C>T
5 human 5583 PRKCH protein kinase C eta XM_024449661.1 76.3% 76.4% 0_1ins483;1191C>T
6 human 5583 PRKCH protein kinase C eta XM_024449662.1 76.3% 76.4% 0_1ins483;1191C>T
7 human 5583 PRKCH protein kinase C eta XM_011536956.1 74.4% 71.7% (many diffs)
8 human 5583 PRKCH protein kinase C eta XM_011536957.1 70.6% 70.1% (many diffs)
9 human 5583 PRKCH protein kinase C eta XM_017021459.1 64.1% 63.2% (many diffs)
10 mouse 18755 Prkch protein kinase C, eta NM_008856.4 89.9% 97.6% (many diffs)
11 mouse 18755 Prkch protein kinase C, eta NM_001313977.1 74.5% 79.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2115
ORF length:
2049
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc gtctggcacc atgaagttca atggctattt gagggtccgc atcggtgagg
121cagtggggct gcagcccacc cgctggtccc tgcgccactc gctcttcaag aagggccacc
181agctgctgga cccctatctg acggtgagcg tggaccaggt gcgcgtgggc cagaccagca
241ccaagcagaa gaccaacaaa cccacgtaca acgaggagtt ttgcgctaac gtcaccgacg
301gcggccacct cgagttggcc gtcttccacg agacgcccct gggctacgac cacttcgtgg
361ccaactgcac cctgcagttc caggagctgc tgcgcacgac cggcgcctcg gacaccttcg
421agggttgggt ggatctcgag ccagagggga aagtattcgt ggtaataacc cttaccggga
481gtttcactga agctactctc cagagagacc ggatcttcaa acattttacc aggaagcgcc
541aaagggctat gcgaaggcga gtccaccaga tcaatggaca caagttcatg gccacgtatc
601tgaggcagcc cacctactgc tctcactgca gggagtttat ctggggagtg tttgggaaac
661agggttatca gtgccaagtg tgcacctgtg tcgtccataa acgctgccat catctaattg
721ttacagcctg tacttgccaa aacaatatta acaaagtgga ttcaaagatt gcagaacaga
781ggttcgggat caacatccca cacaagttca gcatccacaa ctacaaagtg ccaacattct
841gcgatcactg tggctcactg ctctggggaa taatgcgaca aggacttcag tgtaaaatat
901gtaaaatgaa tgtgcatatt cgatgtcaag cgaacgtggc ccctaactgt ggggtaaatg
961cggtggaact tgccaagacc ctggcaggga tgggtctcca acccggaaat atttctccaa
1021cctcgaaact cgtttccaga tcgaccctaa gacgacaggg aaaggagagc agcaaagaag
1081gaaatgggat tggggttaat tcttccaacc gacttggtat cgacaacttt gagttcatcc
1141gagtgttggg gaaggggagt tttgggaagg tgatgcttgc aagagtaaaa gaaacaggag
1201acctctatgc tgtgaaggtg ctgaagaagg acgtgattct gcaggatgat gatgtggaat
1261gcaccatgac cgagaaaagg atcctgtctc tggcccgcaa tcaccccttc ctcactcagt
1321tgttctgctg ctttcagacc cccgatcgtc tgttttttgt gatggagttt gtgaatgggg
1381gtgacttgat gttccacatt cagaagtctc gtcgttttga tgaagcacga gctcgcttct
1441atgctgcaga aatcatttcg gctctcatgt tcctccatga taaaggaatc atctatagag
1501atctgaaact ggacaatgtc ctgttggacc acgagggtca ctgtaaactg gcagacttcg
1561gaatgtgcaa ggaggggatt tgcaatggtg tcaccacggc cacattctgt ggcacgccag
1621actatatcgc tccagagatc ctccaggaaa tgctgtacgg gcctgcagta gactggtggg
1681caatgggcgt gttgctctat gagatgctct gtggtcacgc gccttttgag gcagagaatg
1741aagatgacct ctttgaggcc atactgaatg atgaggtggt ctaccctacc tggctccatg
1801aagatgccac agggatccta aaatctttca tgaccaagaa ccccaccatg cgcttgggca
1861gcctgactca gggaggcgag cacgccatct tgagacatcc tttttttaag gaaatcgacT
1921GGGCCCAGCT GAACCATCGC CAAATAGAAC CGCCTTTCAG ACCCAGAATC AAATCCCGAG
1981AAGATGTCAG TAATTTTGAC CCTGACTTCA TAAAGGAAGA GCCAGTTTTA ACTCCAATTG
2041ATGAGGGACA TCTTCCAATG ATTAACCAGG ATGAGTTTAG AAACTTTTCC TATGTGTCTC
2101CAGAATTGCA ACCATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
2161CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
2221TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATCATCAGAG AAGGCTGTCT
2281TTCTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)