Construct: ORF TRCN0000491934
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008972.1_s317c1
- Derived from:
- ccsbBroadEn_14792
- DNA Barcode:
- ATCATCAGAGAAGGCTGTCTTTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKCH (5583)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491934
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5583 | PRKCH | protein kinase C eta | NM_006255.4 | 99.9% | 100% | 393T>C;1674C>T |
| 2 | human | 5583 | PRKCH | protein kinase C eta | XM_011536954.3 | 86.3% | 83.7% | (many diffs) |
| 3 | human | 5583 | PRKCH | protein kinase C eta | XM_011536955.1 | 83.2% | 82.1% | (many diffs) |
| 4 | human | 5583 | PRKCH | protein kinase C eta | XM_017021458.1 | 76.3% | 76.4% | 0_1ins483;1191C>T |
| 5 | human | 5583 | PRKCH | protein kinase C eta | XM_024449661.1 | 76.3% | 76.4% | 0_1ins483;1191C>T |
| 6 | human | 5583 | PRKCH | protein kinase C eta | XM_024449662.1 | 76.3% | 76.4% | 0_1ins483;1191C>T |
| 7 | human | 5583 | PRKCH | protein kinase C eta | XM_011536956.1 | 74.4% | 71.7% | (many diffs) |
| 8 | human | 5583 | PRKCH | protein kinase C eta | XM_011536957.1 | 70.6% | 70.1% | (many diffs) |
| 9 | human | 5583 | PRKCH | protein kinase C eta | XM_017021459.1 | 64.1% | 63.2% | (many diffs) |
| 10 | mouse | 18755 | Prkch | protein kinase C, eta | NM_008856.4 | 89.9% | 97.6% | (many diffs) |
| 11 | mouse | 18755 | Prkch | protein kinase C, eta | NM_001313977.1 | 74.5% | 79.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2115
- ORF length:
- 2049
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gtctggcacc atgaagttca atggctattt gagggtccgc atcggtgagg 121 cagtggggct gcagcccacc cgctggtccc tgcgccactc gctcttcaag aagggccacc 181 agctgctgga cccctatctg acggtgagcg tggaccaggt gcgcgtgggc cagaccagca 241 ccaagcagaa gaccaacaaa cccacgtaca acgaggagtt ttgcgctaac gtcaccgacg 301 gcggccacct cgagttggcc gtcttccacg agacgcccct gggctacgac cacttcgtgg 361 ccaactgcac cctgcagttc caggagctgc tgcgcacgac cggcgcctcg gacaccttcg 421 agggttgggt ggatctcgag ccagagggga aagtattcgt ggtaataacc cttaccggga 481 gtttcactga agctactctc cagagagacc ggatcttcaa acattttacc aggaagcgcc 541 aaagggctat gcgaaggcga gtccaccaga tcaatggaca caagttcatg gccacgtatc 601 tgaggcagcc cacctactgc tctcactgca gggagtttat ctggggagtg tttgggaaac 661 agggttatca gtgccaagtg tgcacctgtg tcgtccataa acgctgccat catctaattg 721 ttacagcctg tacttgccaa aacaatatta acaaagtgga ttcaaagatt gcagaacaga 781 ggttcgggat caacatccca cacaagttca gcatccacaa ctacaaagtg ccaacattct 841 gcgatcactg tggctcactg ctctggggaa taatgcgaca aggacttcag tgtaaaatat 901 gtaaaatgaa tgtgcatatt cgatgtcaag cgaacgtggc ccctaactgt ggggtaaatg 961 cggtggaact tgccaagacc ctggcaggga tgggtctcca acccggaaat atttctccaa 1021 cctcgaaact cgtttccaga tcgaccctaa gacgacaggg aaaggagagc agcaaagaag 1081 gaaatgggat tggggttaat tcttccaacc gacttggtat cgacaacttt gagttcatcc 1141 gagtgttggg gaaggggagt tttgggaagg tgatgcttgc aagagtaaaa gaaacaggag 1201 acctctatgc tgtgaaggtg ctgaagaagg acgtgattct gcaggatgat gatgtggaat 1261 gcaccatgac cgagaaaagg atcctgtctc tggcccgcaa tcaccccttc ctcactcagt 1321 tgttctgctg ctttcagacc cccgatcgtc tgttttttgt gatggagttt gtgaatgggg 1381 gtgacttgat gttccacatt cagaagtctc gtcgttttga tgaagcacga gctcgcttct 1441 atgctgcaga aatcatttcg gctctcatgt tcctccatga taaaggaatc atctatagag 1501 atctgaaact ggacaatgtc ctgttggacc acgagggtca ctgtaaactg gcagacttcg 1561 gaatgtgcaa ggaggggatt tgcaatggtg tcaccacggc cacattctgt ggcacgccag 1621 actatatcgc tccagagatc ctccaggaaa tgctgtacgg gcctgcagta gactggtggg 1681 caatgggcgt gttgctctat gagatgctct gtggtcacgc gccttttgag gcagagaatg 1741 aagatgacct ctttgaggcc atactgaatg atgaggtggt ctaccctacc tggctccatg 1801 aagatgccac agggatccta aaatctttca tgaccaagaa ccccaccatg cgcttgggca 1861 gcctgactca gggaggcgag cacgccatct tgagacatcc tttttttaag gaaatcgacT 1921 GGGCCCAGCT GAACCATCGC CAAATAGAAC CGCCTTTCAG ACCCAGAATC AAATCCCGAG 1981 AAGATGTCAG TAATTTTGAC CCTGACTTCA TAAAGGAAGA GCCAGTTTTA ACTCCAATTG 2041 ATGAGGGACA TCTTCCAATG ATTAACCAGG ATGAGTTTAG AAACTTTTCC TATGTGTCTC 2101 CAGAATTGCA ACCATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2161 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2221 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATCATCAGAG AAGGCTGTCT 2281 TTCTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt